Staff View: Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital

Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital

Objective: To determine the antibiotic resistance profile (ARP) of Staphylococcus (S.) aureus isolates and molecular typing of the methicillin-resistant S. aureus (MRSA) isolates from Tuanku Mizan Armed Forces Hospital (TMAFH), Kuala Lumpur. Methods: The ARP and presence of the pvl gene were determi...

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Main Authors:	Shakrin, NikNoorul Shakira Mohamed, Junaidi, NorSyaza Syahirah Amat, Huri, MohammadFahmi Daman, Kamarudin, AhmadZakwan, Desa, MohdNasir Mohd, Yunus, WanMohd Zin Wan
Format:	Article
Published:	Wolters Kluwer - Medknow Publications and Media 2023
Online Access:	http://psasir.upm.edu.my/id/eprint/106609/ https://journals.lww.com/aptm/fulltext/2023/16050/antibiotic_resistance_and_molecular_typing_of.4.aspx
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spelling	my.upm.eprints.1066092024-09-26T07:33:38Z http://psasir.upm.edu.my/id/eprint/106609/ Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital Shakrin, NikNoorul Shakira Mohamed Junaidi, NorSyaza Syahirah Amat Huri, MohammadFahmi Daman Kamarudin, AhmadZakwan Desa, MohdNasir Mohd Yunus, WanMohd Zin Wan Objective: To determine the antibiotic resistance profile (ARP) of Staphylococcus (S.) aureus isolates and molecular typing of the methicillin-resistant S. aureus (MRSA) isolates from Tuanku Mizan Armed Forces Hospital (TMAFH), Kuala Lumpur. Methods: The ARP and presence of the pvl gene were determined for 209 S. aureus isolates from clinical specimens. Of these, 123 were methicillin-susceptible S. aureus (MSSA) isolates and 86 were MRSA isolates. All MRSA isolates were characterized using SCCmec typing and spa typing. Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S. aureus isolates. Results: No vancomycin-intermediate and-resistant S. aureus (VISA and VRSA, respectively) were detected among the study isolates. The MSSA isolates showed low resistance rates to all tested antibiotics, were commonly invasive (28/42, 66.7%), and mostly harboured pvl (35/42, 83.3%). Meanwhile, MRSA isolates showed high resistance to penicillin (86/86, 100%), ampicillin (86/86, 100%), sulbactam/ampicillin (86/86, 100%), cefuroxime (81/86, 94.19%), cefoperazone (76/86, 88.37%), azithromycin (56/86, 65.12%), and erythromycin (54/86, 62.79%). The majority of MRSA isolates were of SCCmec type IVh (65/86, 75.58%), spa type t032 (55/85, 63.95%), and grouped into spaCC-T022 (66/85, 77.65%). The t032 type was found to be associated with resistance traits to azithromycin and erythromycin (P<0.05). We also found several spa types that are typically associated with hospital-, community-, and livestock-Associated MRSA co-existing in our MRSA population. Conclusions: This study reflected the consistent absence of VISA and VRSA and corroborated the clonal shifting of MRSA isolates in the Malaysian MRSA isolates. Wolters Kluwer - Medknow Publications and Media 2023-05 Article PeerReviewed Shakrin, NikNoorul Shakira Mohamed and Junaidi, NorSyaza Syahirah Amat and Huri, MohammadFahmi Daman and Kamarudin, AhmadZakwan and Desa, MohdNasir Mohd and Yunus, WanMohd Zin Wan (2023) Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital. Asian Pacific Journal of Tropical Medicine, 16 (5). 220 - 231. ISSN 1995-7645; ESSN: 2352-4146 https://journals.lww.com/aptm/fulltext/2023/16050/antibiotic_resistance_and_molecular_typing_of.4.aspx 10.4103/1995-7645.377743
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Objective: To determine the antibiotic resistance profile (ARP) of Staphylococcus (S.) aureus isolates and molecular typing of the methicillin-resistant S. aureus (MRSA) isolates from Tuanku Mizan Armed Forces Hospital (TMAFH), Kuala Lumpur. Methods: The ARP and presence of the pvl gene were determined for 209 S. aureus isolates from clinical specimens. Of these, 123 were methicillin-susceptible S. aureus (MSSA) isolates and 86 were MRSA isolates. All MRSA isolates were characterized using SCCmec typing and spa typing. Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S. aureus isolates. Results: No vancomycin-intermediate and-resistant S. aureus (VISA and VRSA, respectively) were detected among the study isolates. The MSSA isolates showed low resistance rates to all tested antibiotics, were commonly invasive (28/42, 66.7%), and mostly harboured pvl (35/42, 83.3%). Meanwhile, MRSA isolates showed high resistance to penicillin (86/86, 100%), ampicillin (86/86, 100%), sulbactam/ampicillin (86/86, 100%), cefuroxime (81/86, 94.19%), cefoperazone (76/86, 88.37%), azithromycin (56/86, 65.12%), and erythromycin (54/86, 62.79%). The majority of MRSA isolates were of SCCmec type IVh (65/86, 75.58%), spa type t032 (55/85, 63.95%), and grouped into spaCC-T022 (66/85, 77.65%). The t032 type was found to be associated with resistance traits to azithromycin and erythromycin (P<0.05). We also found several spa types that are typically associated with hospital-, community-, and livestock-Associated MRSA co-existing in our MRSA population. Conclusions: This study reflected the consistent absence of VISA and VRSA and corroborated the clonal shifting of MRSA isolates in the Malaysian MRSA isolates.
format	Article
author	Shakrin, NikNoorul Shakira Mohamed Junaidi, NorSyaza Syahirah Amat Huri, MohammadFahmi Daman Kamarudin, AhmadZakwan Desa, MohdNasir Mohd Yunus, WanMohd Zin Wan
spellingShingle	Shakrin, NikNoorul Shakira Mohamed Junaidi, NorSyaza Syahirah Amat Huri, MohammadFahmi Daman Kamarudin, AhmadZakwan Desa, MohdNasir Mohd Yunus, WanMohd Zin Wan Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
author_facet	Shakrin, NikNoorul Shakira Mohamed Junaidi, NorSyaza Syahirah Amat Huri, MohammadFahmi Daman Kamarudin, AhmadZakwan Desa, MohdNasir Mohd Yunus, WanMohd Zin Wan
author_sort	Shakrin, NikNoorul Shakira Mohamed
title	Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
title_short	Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
title_full	Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
title_fullStr	Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
title_full_unstemmed	Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital
title_sort	antibiotic resistance and molecular typing of clinical staphylococcus aureus isolates from malaysian military hospital
publisher	Wolters Kluwer - Medknow Publications and Media
publishDate	2023
url	http://psasir.upm.edu.my/id/eprint/106609/ https://journals.lww.com/aptm/fulltext/2023/16050/antibiotic_resistance_and_molecular_typing_of.4.aspx
_version_	1811685944447729664
score	13.211869

Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital

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