Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04

This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment...

Full description

Saved in:
Bibliographic Details
Main Authors: Al-Tameemi, A. I., Masarudin, M. J., A. Rahim, R., Timms, V., Neilan, B., Isa, N. M.
Format: Article
Published: University of Baghdad 2023
Online Access:http://psasir.upm.edu.my/id/eprint/106607/
https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1816
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.106607
record_format eprints
spelling my.upm.eprints.1066072024-08-09T03:45:39Z http://psasir.upm.edu.my/id/eprint/106607/ Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04 Al-Tameemi, A. I. Masarudin, M. J. A. Rahim, R. Timms, V. Neilan, B. Isa, N. M. This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment plant in Johor, Malaysia, and was utilized to synthesize ZnO-NPs. The synthesized ZnO-NPs were characterized through several techniques, including UV-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Monodisperse spherical ZnO-NPs of 1.7 - 7.9 nm were obtained with 0.1 M zinc nitrate at 80°C. The biosynthesized ZnO-NPs exhibited vigorous inhibitory activity against MRSA. Results found that ZnO-NPs inhibited MRSA at a minimum concentration of 0.625 mg/mL and were bactericidal at a minimum concentration of 1.25 mg/mL. In MTT assays, ZnO-NPs showed no toxicity to HS-27 fibroblasts. The supernatant of Weissella confusa UPM22MT04 could be used to synthesize ZnO-NPs, which are an antibacterial agent, eco-friendly and nontoxic. University of Baghdad 2023 Article PeerReviewed Al-Tameemi, A. I. and Masarudin, M. J. and A. Rahim, R. and Timms, V. and Neilan, B. and Isa, N. M. (2023) Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04. Iraqi Journal of Agricultural Sciences, 54 (5). 1209 - 1222. ISSN 0075-0530; ESSN: 2410-0862 https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1816 10.36103/ijas.v54i5.1816
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment plant in Johor, Malaysia, and was utilized to synthesize ZnO-NPs. The synthesized ZnO-NPs were characterized through several techniques, including UV-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Monodisperse spherical ZnO-NPs of 1.7 - 7.9 nm were obtained with 0.1 M zinc nitrate at 80°C. The biosynthesized ZnO-NPs exhibited vigorous inhibitory activity against MRSA. Results found that ZnO-NPs inhibited MRSA at a minimum concentration of 0.625 mg/mL and were bactericidal at a minimum concentration of 1.25 mg/mL. In MTT assays, ZnO-NPs showed no toxicity to HS-27 fibroblasts. The supernatant of Weissella confusa UPM22MT04 could be used to synthesize ZnO-NPs, which are an antibacterial agent, eco-friendly and nontoxic.
format Article
author Al-Tameemi, A. I.
Masarudin, M. J.
A. Rahim, R.
Timms, V.
Neilan, B.
Isa, N. M.
spellingShingle Al-Tameemi, A. I.
Masarudin, M. J.
A. Rahim, R.
Timms, V.
Neilan, B.
Isa, N. M.
Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
author_facet Al-Tameemi, A. I.
Masarudin, M. J.
A. Rahim, R.
Timms, V.
Neilan, B.
Isa, N. M.
author_sort Al-Tameemi, A. I.
title Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
title_short Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
title_full Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
title_fullStr Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
title_full_unstemmed Antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of Weissella confusa UPM22MT04
title_sort antibacterial properties of zinc oxide nanoparticles synthesized by the supernatant of weissella confusa upm22mt04
publisher University of Baghdad
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/106607/
https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1816
_version_ 1807051118574829568
score 13.211869