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Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells

Background: Bisphenol A (BPA) is an exogenous endocrine disruptor mimicking hormones closely associated with health complications, such as cancer progression. BPA is also related to an increase in the prevalence of obesity-related diseases due to its obesogenic action. Bombesin-like receptor 3 (BRS3...

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Main Authors: Ismael, Layla Qasim, Keong, Yong Yoke, Bahari, Hasnah, Lan, Chew Ai, Yin, Khoo Boon
Format: Article
Published: Springer Science and Business Media B.V. 2024
Online Access:http://psasir.upm.edu.my/id/eprint/106150/
https://link.springer.com/article/10.1007/s11033-023-09080-2
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spelling my.upm.eprints.1061502024-09-03T03:06:44Z http://psasir.upm.edu.my/id/eprint/106150/ Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells Ismael, Layla Qasim Keong, Yong Yoke Bahari, Hasnah Lan, Chew Ai Yin, Khoo Boon Background: Bisphenol A (BPA) is an exogenous endocrine disruptor mimicking hormones closely associated with health complications, such as cancer progression. BPA is also related to an increase in the prevalence of obesity-related diseases due to its obesogenic action. Bombesin-like receptor 3 (BRS3) is an important factor that should be considered in the adipogenic gene network, as depletion of this gene alters adiposity. Methods: Therefore, the present study aimed to investigate the messenger ribonucleic acid (mRNA) expression of BRS3 in human liver THLE-2 cells post-BPA treatment by real-time polymerase chain reaction. The effects of BPA on the levels of pro-inflammatory proteins, interleukin 6 (IL6) and CC motif chemokine ligand 2 (CCL2), in conditioned media of BPA-treated THLE-2 cells and deoxyribonucleic acid (DNA) synthesis in replicating BPA-treated THLE-2 cells during the cell cycle were also examined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry, respectively. Results: The study found that the mRNA expression of BRS3 was increased in THLE-2 cells treated with BPA. The study also showed that the expression levels of IL6 and CCL2 reached an optimum level in the conditioned media of BPA-treated THLE-2 cells after 48 h of treatment. Subsequently, the DNA synthesis analysis showed that bromodeoxyuridine/propidium iodide (BrdU/PI) stained positive cells were decreased in BPA-treated THLE-2 cells at 72 h of treatment. Conclusion: The study demonstrates that BRS3 expression induced by BPA is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing cellular inflammation in liver cells. © 2024, The Author(s), under exclusive licence to Springer Nature B.V. Springer Science and Business Media B.V. 2024 Article NonPeerReviewed Ismael, Layla Qasim and Keong, Yong Yoke and Bahari, Hasnah and Lan, Chew Ai and Yin, Khoo Boon (2024) Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells. Molecular Biology Reports, 51 (1). ISSN 1573-4978 https://link.springer.com/article/10.1007/s11033-023-09080-2 10.1007/s11033-023-09080-2
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background: Bisphenol A (BPA) is an exogenous endocrine disruptor mimicking hormones closely associated with health complications, such as cancer progression. BPA is also related to an increase in the prevalence of obesity-related diseases due to its obesogenic action. Bombesin-like receptor 3 (BRS3) is an important factor that should be considered in the adipogenic gene network, as depletion of this gene alters adiposity. Methods: Therefore, the present study aimed to investigate the messenger ribonucleic acid (mRNA) expression of BRS3 in human liver THLE-2 cells post-BPA treatment by real-time polymerase chain reaction. The effects of BPA on the levels of pro-inflammatory proteins, interleukin 6 (IL6) and CC motif chemokine ligand 2 (CCL2), in conditioned media of BPA-treated THLE-2 cells and deoxyribonucleic acid (DNA) synthesis in replicating BPA-treated THLE-2 cells during the cell cycle were also examined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry, respectively. Results: The study found that the mRNA expression of BRS3 was increased in THLE-2 cells treated with BPA. The study also showed that the expression levels of IL6 and CCL2 reached an optimum level in the conditioned media of BPA-treated THLE-2 cells after 48 h of treatment. Subsequently, the DNA synthesis analysis showed that bromodeoxyuridine/propidium iodide (BrdU/PI) stained positive cells were decreased in BPA-treated THLE-2 cells at 72 h of treatment. Conclusion: The study demonstrates that BRS3 expression induced by BPA is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing cellular inflammation in liver cells. © 2024, The Author(s), under exclusive licence to Springer Nature B.V.
format Article
author Ismael, Layla Qasim
Keong, Yong Yoke
Bahari, Hasnah
Lan, Chew Ai
Yin, Khoo Boon
spellingShingle Ismael, Layla Qasim
Keong, Yong Yoke
Bahari, Hasnah
Lan, Chew Ai
Yin, Khoo Boon
Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
author_facet Ismael, Layla Qasim
Keong, Yong Yoke
Bahari, Hasnah
Lan, Chew Ai
Yin, Khoo Boon
author_sort Ismael, Layla Qasim
title Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
title_short Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
title_full Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
title_fullStr Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
title_full_unstemmed Bombesin-like receptor 3 expression induced by bisphenol A is likely associated with reduced cell proliferation by inhibiting DNA synthesis and inducing inflammation in liver cells
title_sort bombesin-like receptor 3 expression induced by bisphenol a is likely associated with reduced cell proliferation by inhibiting dna synthesis and inducing inflammation in liver cells
publisher Springer Science and Business Media B.V.
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/106150/
https://link.springer.com/article/10.1007/s11033-023-09080-2
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score 13.211869

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