Staff View: Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)

Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)

The striped flea beetle (FB), Phyllotreta striolata F. (Coleoptera: Chrysomelidae), is not only a serious pest of canoia and mustard but also feed on a wide range of other brassicas. Entomopathogenic fungi (EF) are promising agent for biological control of FB and are gaining increasing attention...

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Bibliographic Details
Main Author:	Priyatno, Tri Puji
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Entomopathogenic fungi - Flea beetles - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10567/1/FP_2001_23.pdf http://psasir.upm.edu.my/id/eprint/10567/
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id	my.upm.eprints.10567
record_format	eprints
spelling	my.upm.eprints.105672024-04-17T03:57:58Z http://psasir.upm.edu.my/id/eprint/10567/ Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae) Priyatno, Tri Puji The striped flea beetle (FB), Phyllotreta striolata F. (Coleoptera: Chrysomelidae), is not only a serious pest of canoia and mustard but also feed on a wide range of other brassicas. Entomopathogenic fungi (EF) are promising agent for biological control of FB and are gaining increasing attention worldwide as mycoinsecticide. The potential of three genera of EF, Metarhizium anisopliae, Beauveria bassiana and Paecilomyces fumosoroseus, has been studied in the laboratory and the field against the striped FB, Phyllotreta strio/ata F. Surveys for FB naturally infected with EF indicated that M anisopliae v. manus and B. bassiana were the potential EF in the populations of FB sampled from vegetable area at UPM's Research Park, Serdang. However, the incidence of infection was very low. Therefore, introduction of virulent isolates into a temporary habitat must be done. Test for pathogenicity of 16 isolates ofEF against adult FB found only one isolate of M anisopliae (MPs) causing mortality in excess of 50%. Four isolates were tested for pathogenicity against the eggs and larvae of the FB. Two isolates of M anisopliae (MPs and Cy3), one B. bassiana (WIs) and one P. fumosoroseus (Pt) were found to be highly pathogenic against the FB larvae while both isolates of M anisopliae were infective against the FB eggs. The resistance of FB adults against EF was caused by the existence of fungistatic compounds on the integument. Five straight chain fatty acids (C4, C6, C7, C8, and C9) suspected as fungistatic compounds based on analysis using Gas Chromatography were proven to inhibit conidial germination. Two media, rice flour and sponge-rice flour medium, examined for conidial massproduction of M anisopliae v. majus and P. fumosoroseus indicated that the spongerice flour medium was shown to be potentially efficient for mass-production of fungal spores. 2001-08 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/10567/1/FP_2001_23.pdf Priyatno, Tri Puji (2001) Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae). Masters thesis, Universiti Putra Malaysia. Entomopathogenic fungi - Flea beetles - Case studies English
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
topic	Entomopathogenic fungi - Flea beetles - Case studies
spellingShingle	Entomopathogenic fungi - Flea beetles - Case studies Priyatno, Tri Puji Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
description	The striped flea beetle (FB), Phyllotreta striolata F. (Coleoptera: Chrysomelidae), is not only a serious pest of canoia and mustard but also feed on a wide range of other brassicas. Entomopathogenic fungi (EF) are promising agent for biological control of FB and are gaining increasing attention worldwide as mycoinsecticide. The potential of three genera of EF, Metarhizium anisopliae, Beauveria bassiana and Paecilomyces fumosoroseus, has been studied in the laboratory and the field against the striped FB, Phyllotreta strio/ata F. Surveys for FB naturally infected with EF indicated that M anisopliae v. manus and B. bassiana were the potential EF in the populations of FB sampled from vegetable area at UPM's Research Park, Serdang. However, the incidence of infection was very low. Therefore, introduction of virulent isolates into a temporary habitat must be done. Test for pathogenicity of 16 isolates ofEF against adult FB found only one isolate of M anisopliae (MPs) causing mortality in excess of 50%. Four isolates were tested for pathogenicity against the eggs and larvae of the FB. Two isolates of M anisopliae (MPs and Cy3), one B. bassiana (WIs) and one P. fumosoroseus (Pt) were found to be highly pathogenic against the FB larvae while both isolates of M anisopliae were infective against the FB eggs. The resistance of FB adults against EF was caused by the existence of fungistatic compounds on the integument. Five straight chain fatty acids (C4, C6, C7, C8, and C9) suspected as fungistatic compounds based on analysis using Gas Chromatography were proven to inhibit conidial germination. Two media, rice flour and sponge-rice flour medium, examined for conidial massproduction of M anisopliae v. majus and P. fumosoroseus indicated that the spongerice flour medium was shown to be potentially efficient for mass-production of fungal spores.
format	Thesis
author	Priyatno, Tri Puji
author_facet	Priyatno, Tri Puji
author_sort	Priyatno, Tri Puji
title	Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
title_short	Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
title_full	Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
title_fullStr	Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
title_full_unstemmed	Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)
title_sort	performance of three genera of entomopathogenic fungi as potential microbial control agents against the flea beetle phyllotreta striolata f. (coleoptera : chrysomelidae)
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/10567/1/FP_2001_23.pdf http://psasir.upm.edu.my/id/eprint/10567/
_version_	1797910839070556160
score	13.211869

Performance of Three Genera of Entomopathogenic Fungi as Potential Microbial Control Agents Against the Flea Beetle Phyllotreta Striolata F. (Coleoptera : Chrysomelidae)

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