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Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract

Mannheimia haemolytica causes pneumonic pasteurellosis (mannheimiosis) which is a common respiratory disease in goats and sheep causing huge economic losses to farmers around the world. In Malaysia, the primary causative agent for small ruminants’ pneumonic mannheimiosis is M. haemolytica serotype A...

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Main Author: Abdirahman, Boorei Mohamed
Format: Thesis
Language:English
Published: 2021
Subjects:
Sheep - Reproduction
Physiology, Pathological
Sheep - Diseases
Online Access:http://psasir.upm.edu.my/id/eprint/104784/1/FPV%202021%2024%20IR.pdf
http://psasir.upm.edu.my/id/eprint/104784/
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spelling my.upm.eprints.1047842023-10-10T07:36:48Z http://psasir.upm.edu.my/id/eprint/104784/ Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract Abdirahman, Boorei Mohamed Mannheimia haemolytica causes pneumonic pasteurellosis (mannheimiosis) which is a common respiratory disease in goats and sheep causing huge economic losses to farmers around the world. In Malaysia, the primary causative agent for small ruminants’ pneumonic mannheimiosis is M. haemolytica serotype A2. The major virulence factors involved in the pathogenesis of pneumonic mannheimiosis are lipopolysaccharide (LPS) and outer membrane protein (OMP). Most of the previous studies were focused on the pathophysiology of the respiratory system in pneumonic goats due to M. haemolytica. It remains unclear whether M. haemolytica has effects on the female reproductive system. Hence, this study was intended to determine the concentration of reproductive hormones (oestrogen and progesterone), pro-inflammatory cytokines (interleukin-1β and interleukin-6), acute phase proteins (haptoglobin and serum amyloid A) and clinical response changes in female goats after challenge with M. haemolytica A2 and its outer membrane protein (OMP). In this study, 12 clinically healthy non-pregnant, cross bred does were used and divided equally into three groups. Does in group 1 (control group) were challenged with 2 ml of sterile phosphate buffered saline (PBS) intranasally, and group 2 does were challenged intranasally with 2 ml of suspension containing 109 colony forming unit (cfu) of M. haemolytica A2. Does in group 3 were challenged with 2ml of OMP intramuscularly extracted from 109 cfu of M. haemolytica A2. Clinical responses were observed following the challenge and blood samples were collected at (0, 1, 2, 4, 6, 12 and 24 hours and at day 3, 7, 21, 35 and 56) post-inoculation for determination of (IL-1β, IL-6, Hp, SAA, oestrogen and progesterone) with sandwich ELISA. The challenged groups showed significant differences (p < 0.05) in rectal temperature, respiratory rate, heart rate and rumen motility compared to the control group. The results of serum analyses revealed that the concentrations of progesterone and oestrogen were significantly decreased (p < 0.05), while the concentrations of pro-inflammatory cytokines (IL-1β and IL-6) and acute phase proteins (Hp and SAA) were significantly increased (p < 0.05) in the challenged groups compared to the control group. The results of histopathological lesions in the lungs, liver, lymph nodes and the reproductive organs revealed mild to moderate significant change (p < 0.05) in both treatment groups compared to the control group. Therefore, these findings suggest that M. haemolytica serotype A2 and its OMP potentially contribute to infertility in female goats and responsible for the occurrence of hormonal imbalances which in turn leads to reproductive inefficiency. 2021-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/104784/1/FPV%202021%2024%20IR.pdf Abdirahman, Boorei Mohamed (2021) Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract. Masters thesis, Universiti Putra Malaysia. Sheep - Reproduction Physiology, Pathological Sheep - Diseases
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Sheep - Reproduction
Physiology, Pathological
Sheep - Diseases
spellingShingle Sheep - Reproduction
Physiology, Pathological
Sheep - Diseases
Abdirahman, Boorei Mohamed
Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
description Mannheimia haemolytica causes pneumonic pasteurellosis (mannheimiosis) which is a common respiratory disease in goats and sheep causing huge economic losses to farmers around the world. In Malaysia, the primary causative agent for small ruminants’ pneumonic mannheimiosis is M. haemolytica serotype A2. The major virulence factors involved in the pathogenesis of pneumonic mannheimiosis are lipopolysaccharide (LPS) and outer membrane protein (OMP). Most of the previous studies were focused on the pathophysiology of the respiratory system in pneumonic goats due to M. haemolytica. It remains unclear whether M. haemolytica has effects on the female reproductive system. Hence, this study was intended to determine the concentration of reproductive hormones (oestrogen and progesterone), pro-inflammatory cytokines (interleukin-1β and interleukin-6), acute phase proteins (haptoglobin and serum amyloid A) and clinical response changes in female goats after challenge with M. haemolytica A2 and its outer membrane protein (OMP). In this study, 12 clinically healthy non-pregnant, cross bred does were used and divided equally into three groups. Does in group 1 (control group) were challenged with 2 ml of sterile phosphate buffered saline (PBS) intranasally, and group 2 does were challenged intranasally with 2 ml of suspension containing 109 colony forming unit (cfu) of M. haemolytica A2. Does in group 3 were challenged with 2ml of OMP intramuscularly extracted from 109 cfu of M. haemolytica A2. Clinical responses were observed following the challenge and blood samples were collected at (0, 1, 2, 4, 6, 12 and 24 hours and at day 3, 7, 21, 35 and 56) post-inoculation for determination of (IL-1β, IL-6, Hp, SAA, oestrogen and progesterone) with sandwich ELISA. The challenged groups showed significant differences (p < 0.05) in rectal temperature, respiratory rate, heart rate and rumen motility compared to the control group. The results of serum analyses revealed that the concentrations of progesterone and oestrogen were significantly decreased (p < 0.05), while the concentrations of pro-inflammatory cytokines (IL-1β and IL-6) and acute phase proteins (Hp and SAA) were significantly increased (p < 0.05) in the challenged groups compared to the control group. The results of histopathological lesions in the lungs, liver, lymph nodes and the reproductive organs revealed mild to moderate significant change (p < 0.05) in both treatment groups compared to the control group. Therefore, these findings suggest that M. haemolytica serotype A2 and its OMP potentially contribute to infertility in female goats and responsible for the occurrence of hormonal imbalances which in turn leads to reproductive inefficiency.
format Thesis
author Abdirahman, Boorei Mohamed
author_facet Abdirahman, Boorei Mohamed
author_sort Abdirahman, Boorei Mohamed
title Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
title_short Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
title_full Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
title_fullStr Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
title_full_unstemmed Reproductive pathophysiology of non-pregnant does challenged with Mannheimia haemolytica serotype A2 and its outer membrane protein extract
title_sort reproductive pathophysiology of non-pregnant does challenged with mannheimia haemolytica serotype a2 and its outer membrane protein extract
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/104784/1/FPV%202021%2024%20IR.pdf
http://psasir.upm.edu.my/id/eprint/104784/
_version_ 1781706743243341824
score 13.211869

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