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Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia

Free-roaming and scavenging lifestyles of Rattus spp. in densely populated urban areas expose them to multiple pathogens that facilitate the transmission of infection to the human population more rapidly, raising public health concerns. There is limited information on the status of rat susceptibilit...

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Main Authors: Natasha, Jafar Ali, Yasmin, Abd Rahaman, Siti-Maisarah, Abd Manan, Nur-Anis, Zakaria, Tharshaini, Murugasu, Arshad, Siti Suri, Ayuni, Wan Noor, Mohammed, Mohammed Nma, Nur-Fazila, Saulol Hamid
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2022
Online Access:http://psasir.upm.edu.my/id/eprint/103144/1/103144.pdf
http://psasir.upm.edu.my/id/eprint/103144/
https://www.cabidigitallibrary.org/doi/pdf/10.5555/20230080715
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spelling my.upm.eprints.1031442024-11-25T03:11:20Z http://psasir.upm.edu.my/id/eprint/103144/ Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia Natasha, Jafar Ali Yasmin, Abd Rahaman Siti-Maisarah, Abd Manan Nur-Anis, Zakaria Tharshaini, Murugasu Arshad, Siti Suri Ayuni, Wan Noor Mohammed, Mohammed Nma Nur-Fazila, Saulol Hamid Free-roaming and scavenging lifestyles of Rattus spp. in densely populated urban areas expose them to multiple pathogens that facilitate the transmission of infection to the human population more rapidly, raising public health concerns. There is limited information on the status of rat susceptibility to virus infection, particularly West Nile virus (WNV), herpesvirus, and parvovirus, to prepare for emerging zoonosis. A total of 23 (n = 23) blood samples collected from Rattus spp. in the wet market areas of Klang Valley, Malaysia, were subjected to molecular assay using a one-step reverse transcription-polymerase chain reaction (RTPCR) to detect the highly conserved region of the WNV capsid and pre-membrane protein via nested polymerase chain reaction (PCR) assay targeting highly conserved aminoacid motifs within the herpesviral DNAdirected DNA polymerase gene (DPOL)and polymerase chain reaction (PCR) assay targeting the parvovirus non-structural (NS) protein. As a result, 4 out of 23 (17.39%) rats were positive for herpesvirus DNA, but none were positive for WNV RNA and parvovirus DNA. The positive PCR amplicons of herpesvirus DNA were subjected to partial DNA sequencing analysis, 100% identical to Acomys herpesvirus SVMS 226,222 from Betaherpesvirinae, which is highly suggestive of rat cytomegalovirus (RCMV). This study has successfully demonstrated the presence of RCMV from Rattus spp. in the Klang Valley. The RCMV potentially crosses species barriers and establishes infection, raising public health concerns. The non-viraemic state of WNV or parvovirus infection, low sample size, and limited niche distribution emphasise the need for the expansion of this study in the future. Universiti Putra Malaysia Press 2022 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/103144/1/103144.pdf Natasha, Jafar Ali and Yasmin, Abd Rahaman and Siti-Maisarah, Abd Manan and Nur-Anis, Zakaria and Tharshaini, Murugasu and Arshad, Siti Suri and Ayuni, Wan Noor and Mohammed, Mohammed Nma and Nur-Fazila, Saulol Hamid (2022) Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia. Pertanika Journal of Tropical Agricultural Science, 45 (4). 1113 - 1124. ISSN 1511-3701; eISSN: 2231-8542 https://www.cabidigitallibrary.org/doi/pdf/10.5555/20230080715 10.47836/pjtas.45.4.15
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Free-roaming and scavenging lifestyles of Rattus spp. in densely populated urban areas expose them to multiple pathogens that facilitate the transmission of infection to the human population more rapidly, raising public health concerns. There is limited information on the status of rat susceptibility to virus infection, particularly West Nile virus (WNV), herpesvirus, and parvovirus, to prepare for emerging zoonosis. A total of 23 (n = 23) blood samples collected from Rattus spp. in the wet market areas of Klang Valley, Malaysia, were subjected to molecular assay using a one-step reverse transcription-polymerase chain reaction (RTPCR) to detect the highly conserved region of the WNV capsid and pre-membrane protein via nested polymerase chain reaction (PCR) assay targeting highly conserved aminoacid motifs within the herpesviral DNAdirected DNA polymerase gene (DPOL)and polymerase chain reaction (PCR) assay targeting the parvovirus non-structural (NS) protein. As a result, 4 out of 23 (17.39%) rats were positive for herpesvirus DNA, but none were positive for WNV RNA and parvovirus DNA. The positive PCR amplicons of herpesvirus DNA were subjected to partial DNA sequencing analysis, 100% identical to Acomys herpesvirus SVMS 226,222 from Betaherpesvirinae, which is highly suggestive of rat cytomegalovirus (RCMV). This study has successfully demonstrated the presence of RCMV from Rattus spp. in the Klang Valley. The RCMV potentially crosses species barriers and establishes infection, raising public health concerns. The non-viraemic state of WNV or parvovirus infection, low sample size, and limited niche distribution emphasise the need for the expansion of this study in the future.
format Article
author Natasha, Jafar Ali
Yasmin, Abd Rahaman
Siti-Maisarah, Abd Manan
Nur-Anis, Zakaria
Tharshaini, Murugasu
Arshad, Siti Suri
Ayuni, Wan Noor
Mohammed, Mohammed Nma
Nur-Fazila, Saulol Hamid
spellingShingle Natasha, Jafar Ali
Yasmin, Abd Rahaman
Siti-Maisarah, Abd Manan
Nur-Anis, Zakaria
Tharshaini, Murugasu
Arshad, Siti Suri
Ayuni, Wan Noor
Mohammed, Mohammed Nma
Nur-Fazila, Saulol Hamid
Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
author_facet Natasha, Jafar Ali
Yasmin, Abd Rahaman
Siti-Maisarah, Abd Manan
Nur-Anis, Zakaria
Tharshaini, Murugasu
Arshad, Siti Suri
Ayuni, Wan Noor
Mohammed, Mohammed Nma
Nur-Fazila, Saulol Hamid
author_sort Natasha, Jafar Ali
title Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
title_short Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
title_full Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
title_fullStr Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
title_full_unstemmed Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia
title_sort screening of west nile virus, herpesvirus, and parvovirus in rattus spp. in klang valley, malaysia
publisher Universiti Putra Malaysia Press
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/103144/1/103144.pdf
http://psasir.upm.edu.my/id/eprint/103144/
https://www.cabidigitallibrary.org/doi/pdf/10.5555/20230080715
_version_ 1817844592064593920
score 13.223943

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