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Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia

Pathogenic Acanthamoeba species are the causative agents of Acanthamoeba keratitis and granulomatous amoebic encephalitis. Members of this amoeba genus are ubiquitous in the environments. In Malaysia, most environmental studies performed to date have targeted the detection and characterisation of Ac...

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Main Authors: Chan, Li-Li, Toh, Hiu-Ching, Jaikwang, Kantiya, Loo, Ee-Mun, Wong, Jia-Haw, Liew, Yun-Khoon, Ong, Kian-Huat, Ooi, Soo-Shen
Format: Article
Published: Elsevier 2022
Online Access:http://psasir.upm.edu.my/id/eprint/103055/
https://linkinghub.elsevier.com/retrieve/pii/S0001706X22000183
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spelling my.upm.eprints.1030552024-06-30T23:39:19Z http://psasir.upm.edu.my/id/eprint/103055/ Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia Chan, Li-Li Toh, Hiu-Ching Jaikwang, Kantiya Loo, Ee-Mun Wong, Jia-Haw Liew, Yun-Khoon Ong, Kian-Huat Ooi, Soo-Shen Pathogenic Acanthamoeba species are the causative agents of Acanthamoeba keratitis and granulomatous amoebic encephalitis. Members of this amoeba genus are ubiquitous in the environments. In Malaysia, most environmental studies performed to date have targeted the detection and characterisation of Acanthamoeba sp. in different water sources, dust and soil samples collected near human habitats. However, no local study has yet to examine these amoebae in a forest, an isolated terrestrial environment, where human activity is relatively scarce. Further, there are also limited studies to investigate the same globally. The current study reported the isolation, morphological and genotypic characterisations of eleven Acanthamoeba sp. isolated from soils of the Payeh Maga Highland forest, Sarawak, Malaysia. Morphological analysis revealed that nine isolates belonged to Group II, whereas two isolates belonged to Group III as defined by the criteria of Pussard and Pons. The phylogenetic analysis based on complete 18S rRNA gene sequences showed that the isolates belonged to the rare T1 (six isolates), T6 (two isolates) and T13 (three isolates) genotypes. To the best of our knowledge, this is the first report about the detection of T6 Acanthamoeba sp. in this country. Overall, the current findings have enriched the knowledge pertaining to the occurrence, morphological and genotypic characteristics of Acanthamoeba sp. in an isolated terrestrial environment of Malaysia. Elsevier 2022 Article PeerReviewed Chan, Li-Li and Toh, Hiu-Ching and Jaikwang, Kantiya and Loo, Ee-Mun and Wong, Jia-Haw and Liew, Yun-Khoon and Ong, Kian-Huat and Ooi, Soo-Shen (2022) Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia. Acta Tropica, 229. art. no. 106372. pp. 1-9. ISSN 0001-706X https://linkinghub.elsevier.com/retrieve/pii/S0001706X22000183 10.1016/j.actatropica.2022.106372
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Pathogenic Acanthamoeba species are the causative agents of Acanthamoeba keratitis and granulomatous amoebic encephalitis. Members of this amoeba genus are ubiquitous in the environments. In Malaysia, most environmental studies performed to date have targeted the detection and characterisation of Acanthamoeba sp. in different water sources, dust and soil samples collected near human habitats. However, no local study has yet to examine these amoebae in a forest, an isolated terrestrial environment, where human activity is relatively scarce. Further, there are also limited studies to investigate the same globally. The current study reported the isolation, morphological and genotypic characterisations of eleven Acanthamoeba sp. isolated from soils of the Payeh Maga Highland forest, Sarawak, Malaysia. Morphological analysis revealed that nine isolates belonged to Group II, whereas two isolates belonged to Group III as defined by the criteria of Pussard and Pons. The phylogenetic analysis based on complete 18S rRNA gene sequences showed that the isolates belonged to the rare T1 (six isolates), T6 (two isolates) and T13 (three isolates) genotypes. To the best of our knowledge, this is the first report about the detection of T6 Acanthamoeba sp. in this country. Overall, the current findings have enriched the knowledge pertaining to the occurrence, morphological and genotypic characteristics of Acanthamoeba sp. in an isolated terrestrial environment of Malaysia.
format Article
author Chan, Li-Li
Toh, Hiu-Ching
Jaikwang, Kantiya
Loo, Ee-Mun
Wong, Jia-Haw
Liew, Yun-Khoon
Ong, Kian-Huat
Ooi, Soo-Shen
spellingShingle Chan, Li-Li
Toh, Hiu-Ching
Jaikwang, Kantiya
Loo, Ee-Mun
Wong, Jia-Haw
Liew, Yun-Khoon
Ong, Kian-Huat
Ooi, Soo-Shen
Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
author_facet Chan, Li-Li
Toh, Hiu-Ching
Jaikwang, Kantiya
Loo, Ee-Mun
Wong, Jia-Haw
Liew, Yun-Khoon
Ong, Kian-Huat
Ooi, Soo-Shen
author_sort Chan, Li-Li
title Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
title_short Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
title_full Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
title_fullStr Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
title_full_unstemmed Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
title_sort report of rare genotypes of acanthamoeba from soil source of the payeh maga highland forest, north-eastern sarawak, malaysia
publisher Elsevier
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/103055/
https://linkinghub.elsevier.com/retrieve/pii/S0001706X22000183
_version_ 1804067029152432128
score 13.211869

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