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Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents

Bougainvillea, popularly known as ‘Bunga kertas’ in Malaysia, is thoroughly explored for nutritional and medicinal purposes. Bougainvillea has been shown to possess alkaloids and flavonoids which are widely used in folk medicine to treat different illnesses such as inflammatory, diarrheal, ulcer, an...

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Main Authors: Nasrat, Mohammad Nasim, Sakimin, Siti Zaharah, Hakiman, Mansor
Format: Article
Published: MDPI 2022
Online Access:http://psasir.upm.edu.my/id/eprint/102780/
https://www.mdpi.com/2311-7524/8/8/712
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spelling my.upm.eprints.1027802024-06-22T14:11:53Z http://psasir.upm.edu.my/id/eprint/102780/ Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents Nasrat, Mohammad Nasim Sakimin, Siti Zaharah Hakiman, Mansor Bougainvillea, popularly known as ‘Bunga kertas’ in Malaysia, is thoroughly explored for nutritional and medicinal purposes. Bougainvillea has been shown to possess alkaloids and flavonoids which are widely used in folk medicine to treat different illnesses such as inflammatory, diarrheal, ulcer, and diabetic. Despite its major conventional therapeutic importance, only limited attempts have been made to investigate this species’ chemical and pharmacological properties in relation to its medicinal uses. Therefore, this study was conducted to determine the effect of in vitro-induced callus under different light conditions and plant growth regulators on phytochemical and antioxidant activities using different extraction solvents. Based on the results, the maximum days (17.67) to callus initiation were recorded when nodal was cultured on woody plant medium (WPM) supplemented with 7.5 µM 2,4-D + 0.5 µM BAP under light condition. On the contrary, the minimum days (7) to callus initiation were obtained when nodal was treated with 2.5 and 5 µM 2,4-D + 1 and 1.5 µM BAP under dark conditions. However, higher fresh and dry weight of callus was obtained when nodal was cultured on woody plant medium fortified with 7.5 µM 2,4-D + 1.5 µM BAP under dark and light conditions. In the analysis of the phenolics content and antioxidant activities, aqueous extract of conventionally propagated nodal part exhibited the highest phenolic content and antioxidant activities. However, the highest iron (II) chelating activity was produced from the aqueous extract of the calli induced under a dark condition. Hence, it can be concluded that the callus culture of Bougainvillea produced plant secondary metabolites and antioxidant activities comparable to the mother plants. MDPI 2022-08 Article PeerReviewed Nasrat, Mohammad Nasim and Sakimin, Siti Zaharah and Hakiman, Mansor (2022) Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents. Horticulturae, 8 (8). art. no. 712. pp. 1-18. ISSN 2311-7524 https://www.mdpi.com/2311-7524/8/8/712 10.3390/horticulturae8080712
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Bougainvillea, popularly known as ‘Bunga kertas’ in Malaysia, is thoroughly explored for nutritional and medicinal purposes. Bougainvillea has been shown to possess alkaloids and flavonoids which are widely used in folk medicine to treat different illnesses such as inflammatory, diarrheal, ulcer, and diabetic. Despite its major conventional therapeutic importance, only limited attempts have been made to investigate this species’ chemical and pharmacological properties in relation to its medicinal uses. Therefore, this study was conducted to determine the effect of in vitro-induced callus under different light conditions and plant growth regulators on phytochemical and antioxidant activities using different extraction solvents. Based on the results, the maximum days (17.67) to callus initiation were recorded when nodal was cultured on woody plant medium (WPM) supplemented with 7.5 µM 2,4-D + 0.5 µM BAP under light condition. On the contrary, the minimum days (7) to callus initiation were obtained when nodal was treated with 2.5 and 5 µM 2,4-D + 1 and 1.5 µM BAP under dark conditions. However, higher fresh and dry weight of callus was obtained when nodal was cultured on woody plant medium fortified with 7.5 µM 2,4-D + 1.5 µM BAP under dark and light conditions. In the analysis of the phenolics content and antioxidant activities, aqueous extract of conventionally propagated nodal part exhibited the highest phenolic content and antioxidant activities. However, the highest iron (II) chelating activity was produced from the aqueous extract of the calli induced under a dark condition. Hence, it can be concluded that the callus culture of Bougainvillea produced plant secondary metabolites and antioxidant activities comparable to the mother plants.
format Article
author Nasrat, Mohammad Nasim
Sakimin, Siti Zaharah
Hakiman, Mansor
spellingShingle Nasrat, Mohammad Nasim
Sakimin, Siti Zaharah
Hakiman, Mansor
Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
author_facet Nasrat, Mohammad Nasim
Sakimin, Siti Zaharah
Hakiman, Mansor
author_sort Nasrat, Mohammad Nasim
title Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
title_short Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
title_full Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
title_fullStr Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
title_full_unstemmed Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents
title_sort phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of bougainvillea glabra choisy using different solvents
publisher MDPI
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/102780/
https://www.mdpi.com/2311-7524/8/8/712
_version_ 1802978821908987904
score 13.211869

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