Cover Image

Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents

Artemisia verlotiorum Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher...

Full description

Saved in:
Bibliographic Details
Main Authors: Suroowan, Shanoo, Llorent-Martínez, Eulogio Jose, Zengin, Gokhan, Dall’acqua, Stefano, Sut, Stefania, Buskaran, Kalaivani, Fakurazi, Sharida, Mahomoodally, Mohamad Fawzi
Format: Article
Published: MDPI 2022
Online Access:http://psasir.upm.edu.my/id/eprint/102778/
https://www.mdpi.com/1420-3049/27/18/5886
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.102778
record_format eprints
spelling my.upm.eprints.1027782024-06-22T14:08:18Z http://psasir.upm.edu.my/id/eprint/102778/ Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents Suroowan, Shanoo Llorent-Martínez, Eulogio Jose Zengin, Gokhan Dall’acqua, Stefano Sut, Stefania Buskaran, Kalaivani Fakurazi, Sharida Mahomoodally, Mohamad Fawzi Artemisia verlotiorum Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher the phytochemicals; quantify through HPLC-ESI-MS analysis the plant’s biosynthesis; and evaluate the antioxidant, anti-tyrosinase, amylase, glucosidase, cholinesterase, and cytotoxicity potential on normal (NIH 3T3) and human liver and human colon cancer (HepG2 and HT 29) cell lines of this plant species. The aqueous extract contained the highest content of phenolics and phenolic acid, methanol extracted the most flavonoid, and the most flavonol was extracted by ethyl acetate. The one-way ANOVA results demonstrated that all results obtained were statistically significant at p < 0.05. A total of 25 phytoconstituents were identified from the different extracts, with phenolic acids and flavonoids being the main metabolites. The highest antioxidant potential was recorded for the aqueous extract. The best anti-tyrosinase extract was the methanolic extract. The ethyl acetate extract of A. verlotiorum had the highest flavonol content and hence was most active against the cholinesterase enzymes. The ethyl acetate extract was the best α-glucosidase and α-amylase inhibitor. The samples of Artemisia verlotiorum Lamotte in both aqueous and methanolic extracts were found to be non-toxic after 48 h against NIH 3T3 cells. In HepG2 cells, the methanolic extract was nontoxic up to 125 µg/mL, and an IC50 value of 722.39 µg/mL was recorded. The IC50 value exhibited in methanolic extraction of A. verlotiorum was 792.91 µg/mL in HT29 cells. Methanolic extraction is capable of inducing cell cytotoxicity in human hepatocellular carcinoma without damaging normal cells. Hence, A. verlotiorum can be recommended for further evaluation of its phytochemical and medicinal properties. MDPI 2022 Article PeerReviewed Suroowan, Shanoo and Llorent-Martínez, Eulogio Jose and Zengin, Gokhan and Dall’acqua, Stefano and Sut, Stefania and Buskaran, Kalaivani and Fakurazi, Sharida and Mahomoodally, Mohamad Fawzi (2022) Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents. Molecules, 27 (18). art. no. 5886. pp. 1-21. ISSN 1420-3049 https://www.mdpi.com/1420-3049/27/18/5886 10.3390/molecules27185886
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Artemisia verlotiorum Lamotte is recognized medicinally given its long-standing ethnopharmacological uses in different parts of the world. Nonetheless, the pharmacological properties of the leaves of the plant have been poorly studied by the scientific community. Hence, this study aimed to decipher the phytochemicals; quantify through HPLC-ESI-MS analysis the plant’s biosynthesis; and evaluate the antioxidant, anti-tyrosinase, amylase, glucosidase, cholinesterase, and cytotoxicity potential on normal (NIH 3T3) and human liver and human colon cancer (HepG2 and HT 29) cell lines of this plant species. The aqueous extract contained the highest content of phenolics and phenolic acid, methanol extracted the most flavonoid, and the most flavonol was extracted by ethyl acetate. The one-way ANOVA results demonstrated that all results obtained were statistically significant at p < 0.05. A total of 25 phytoconstituents were identified from the different extracts, with phenolic acids and flavonoids being the main metabolites. The highest antioxidant potential was recorded for the aqueous extract. The best anti-tyrosinase extract was the methanolic extract. The ethyl acetate extract of A. verlotiorum had the highest flavonol content and hence was most active against the cholinesterase enzymes. The ethyl acetate extract was the best α-glucosidase and α-amylase inhibitor. The samples of Artemisia verlotiorum Lamotte in both aqueous and methanolic extracts were found to be non-toxic after 48 h against NIH 3T3 cells. In HepG2 cells, the methanolic extract was nontoxic up to 125 µg/mL, and an IC50 value of 722.39 µg/mL was recorded. The IC50 value exhibited in methanolic extraction of A. verlotiorum was 792.91 µg/mL in HT29 cells. Methanolic extraction is capable of inducing cell cytotoxicity in human hepatocellular carcinoma without damaging normal cells. Hence, A. verlotiorum can be recommended for further evaluation of its phytochemical and medicinal properties.
format Article
author Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Mahomoodally, Mohamad Fawzi
spellingShingle Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Mahomoodally, Mohamad Fawzi
Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
author_facet Suroowan, Shanoo
Llorent-Martínez, Eulogio Jose
Zengin, Gokhan
Dall’acqua, Stefano
Sut, Stefania
Buskaran, Kalaivani
Fakurazi, Sharida
Mahomoodally, Mohamad Fawzi
author_sort Suroowan, Shanoo
title Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
title_short Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
title_full Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
title_fullStr Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
title_full_unstemmed Phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of Artemisia verlotiorum Lamotte extracts: a new source of bioactive agents
title_sort phytochemical characterization, anti-oxidant, anti-enzymatic and cytotoxic effects of artemisia verlotiorum lamotte extracts: a new source of bioactive agents
publisher MDPI
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/102778/
https://www.mdpi.com/1420-3049/27/18/5886
_version_ 1802978821767430144
score 13.211869

Similar Items