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Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)

Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit...

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Main Author: Krishnapillay, Baskaran
Format: Thesis
Language:English
English
Published: 1989
Online Access:http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf
http://psasir.upm.edu.my/id/eprint/10269/
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spelling my.upm.eprints.102692011-03-21T02:17:46Z http://psasir.upm.edu.my/id/eprint/10269/ Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.) Krishnapillay, Baskaran Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit maturity was critical in the study where embryos excised from mature unripe fruits were found to be the most appropriate. Selection of uniform sized embryos (4.0 - 5.0 m m in length) minimised variability in embryo fresh weight and moisture content to a considerable extent. A modified Murashige and Skoog medium containing 1.0 mg/l each of NAA and BAP, 2 gil of activated charcoal and 170 mg/l of NaH2 P04. H20 was found to be a suitable medium for development and growth of the excised jackfruit embryos. The safe critical moisture limit for excised jackfruit embryos was found to be around 14-15%. Storage of embryos dried to this moisture limit by conventional storage methods with a view to long term storage was unsuccessful. The maximum storage period achieved was only one month. Attempts at direct storage in liquid nitrogen after desiccation to near critical moisture limit was also found to be unsuccessful. 1989 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf Krishnapillay, Baskaran (1989) Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.). PhD thesis, Universiti Pertanian Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit maturity was critical in the study where embryos excised from mature unripe fruits were found to be the most appropriate. Selection of uniform sized embryos (4.0 - 5.0 m m in length) minimised variability in embryo fresh weight and moisture content to a considerable extent. A modified Murashige and Skoog medium containing 1.0 mg/l each of NAA and BAP, 2 gil of activated charcoal and 170 mg/l of NaH2 P04. H20 was found to be a suitable medium for development and growth of the excised jackfruit embryos. The safe critical moisture limit for excised jackfruit embryos was found to be around 14-15%. Storage of embryos dried to this moisture limit by conventional storage methods with a view to long term storage was unsuccessful. The maximum storage period achieved was only one month. Attempts at direct storage in liquid nitrogen after desiccation to near critical moisture limit was also found to be unsuccessful.
format Thesis
author Krishnapillay, Baskaran
spellingShingle Krishnapillay, Baskaran
Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
author_facet Krishnapillay, Baskaran
author_sort Krishnapillay, Baskaran
title Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_short Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_full Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_fullStr Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_full_unstemmed Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_sort towards the development of a protocol for cryopreservation of embryos of a recalcitrant seed (artocarpus heterophyllus lam.)
publishDate 1989
url http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf
http://psasir.upm.edu.my/id/eprint/10269/
_version_ 1643824555359207424
score 13.211869

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