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Versatility of subtilisin: a review on structure, characteristics, and applications

Due to its thermostability and high pH compatibility, subtilisin is most known for its role as an additive for detergents in which it is categorized as a serine protease according to MEROPS database. Subtilisin is typically isolated from various bacterial species of the Bacillus genus such as Bacill...

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Main Authors:	Mohd Azrin, Nur Aliyah, Mohamad Ali, Mohd Shukuri, Raja Abd Rahman, Raja Noor Zaliha, Oslan, Siti Nurbaya, Muhd Noor, Noor Dina
Format:	Article
Published:	John Wiley & Sons, Inc. 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/102589/ https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2309
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spelling	my.upm.eprints.1025892024-03-12T04:18:34Z http://psasir.upm.edu.my/id/eprint/102589/ Versatility of subtilisin: a review on structure, characteristics, and applications Mohd Azrin, Nur Aliyah Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Oslan, Siti Nurbaya Muhd Noor, Noor Dina Due to its thermostability and high pH compatibility, subtilisin is most known for its role as an additive for detergents in which it is categorized as a serine protease according to MEROPS database. Subtilisin is typically isolated from various bacterial species of the Bacillus genus such as Bacillus subtilis, B. amyloliquefaciens, B. licheniformis, and various other organisms. It is composed of 268–275 amino acid residues and is initially secreted in the precursor form, preprosubtilisin, which is composed of 29-residues signal peptide, 77-residues propeptide, and 275-residues active subtilisin. Subtilisin is known for the presence of high and low affinity calcium binding sites in its structure. Native subtilisin has general properties of thermostability, tolerance to neutral to high pH, broad specificity, and calcium-dependent stability, which contribute to the versatility of subtilisin applicability. Through protein engineering and immobilization technologies, many variants of subtilisin have been generated, which increase the applicability of subtilisin in various industries including detergent, food processing and packaging, synthesis of inhibitory peptides, therapeutic, and waste management applications. John Wiley & Sons, Inc. 2022-12 Article PeerReviewed Mohd Azrin, Nur Aliyah and Mohamad Ali, Mohd Shukuri and Raja Abd Rahman, Raja Noor Zaliha and Oslan, Siti Nurbaya and Muhd Noor, Noor Dina (2022) Versatility of subtilisin: a review on structure, characteristics, and applications. Biotechnology and Applied Biochemistry, 69 (6). pp. 2599-2616. ISSN 0885-4513; ESSN: 1470-8744 https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2309 10.1002/bab.2309
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Due to its thermostability and high pH compatibility, subtilisin is most known for its role as an additive for detergents in which it is categorized as a serine protease according to MEROPS database. Subtilisin is typically isolated from various bacterial species of the Bacillus genus such as Bacillus subtilis, B. amyloliquefaciens, B. licheniformis, and various other organisms. It is composed of 268–275 amino acid residues and is initially secreted in the precursor form, preprosubtilisin, which is composed of 29-residues signal peptide, 77-residues propeptide, and 275-residues active subtilisin. Subtilisin is known for the presence of high and low affinity calcium binding sites in its structure. Native subtilisin has general properties of thermostability, tolerance to neutral to high pH, broad specificity, and calcium-dependent stability, which contribute to the versatility of subtilisin applicability. Through protein engineering and immobilization technologies, many variants of subtilisin have been generated, which increase the applicability of subtilisin in various industries including detergent, food processing and packaging, synthesis of inhibitory peptides, therapeutic, and waste management applications.
format	Article
author	Mohd Azrin, Nur Aliyah Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Oslan, Siti Nurbaya Muhd Noor, Noor Dina
spellingShingle	Mohd Azrin, Nur Aliyah Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Oslan, Siti Nurbaya Muhd Noor, Noor Dina Versatility of subtilisin: a review on structure, characteristics, and applications
author_facet	Mohd Azrin, Nur Aliyah Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Oslan, Siti Nurbaya Muhd Noor, Noor Dina
author_sort	Mohd Azrin, Nur Aliyah
title	Versatility of subtilisin: a review on structure, characteristics, and applications
title_short	Versatility of subtilisin: a review on structure, characteristics, and applications
title_full	Versatility of subtilisin: a review on structure, characteristics, and applications
title_fullStr	Versatility of subtilisin: a review on structure, characteristics, and applications
title_full_unstemmed	Versatility of subtilisin: a review on structure, characteristics, and applications
title_sort	versatility of subtilisin: a review on structure, characteristics, and applications
publisher	John Wiley & Sons, Inc.
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/102589/ https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2309
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score	13.211869

Versatility of subtilisin: a review on structure, characteristics, and applications

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