Staff View: Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves

Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves

Mycoplasma bovis (M. bovis) is a pathogen that especially effects the respiratory system of cattle. We focused on the histopathological changes in the lungs caused by M. bovis because the lesions in the bronchi or bronchioles and alveoli are quite different in natural infections. Thirty-five lung sa...

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Main Authors:	Suwanruengsri, Mathurot, Uemura, Ryoko, Uda Zahli, Izzati, Kanda, Takuya, Fuke, Naoyuki, Yasuda, Masahiro, Hirai, Takuya, Yamaguchi, Ryoji
Format:	Article
Published:	Chulalongkorn University 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/102288/ https://he01.tci-thaijo.org/index.php/tjvm/article/view/254775
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spelling	my.upm.eprints.1022882023-09-21T04:00:47Z http://psasir.upm.edu.my/id/eprint/102288/ Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves Suwanruengsri, Mathurot Uemura, Ryoko Uda Zahli, Izzati Kanda, Takuya Fuke, Naoyuki Yasuda, Masahiro Hirai, Takuya Yamaguchi, Ryoji Mycoplasma bovis (M. bovis) is a pathogen that especially effects the respiratory system of cattle. We focused on the histopathological changes in the lungs caused by M. bovis because the lesions in the bronchi or bronchioles and alveoli are quite different in natural infections. Thirty-five lung samples from 2-to 12-month-old Japanese Black calves with respiratory symptoms were collected and examined for bacteria presence and pathological changes using immunohistochemistry (IHC). Using the loop-mediated isothermal amplification method, 18 of the 35 samples were found to be positive for M. bovis, which was isolated and identified. In 7 of the samples, only M. bovis was detected, whereas in the other 11, other bacteria coexisted with M. bovis. Anti-M. bovis, anti-MAC387, anti-Iba1, anti-CD3, anti-CD20, anti-AE1/AE3 cytokeratin and anti-IL8 were used for antigen detection. Histopathological studies and diagnoses of the lungs were examined in two parts; bronchiolar lesions and alveolar lesions. The bronchiolar findings were divided into three groups: granulomatous, caseonecrotic and suppurative bronchiolitis. The alveolar lesions found were quite mild, except in three cases. IHC staining revealed strong evidence of M. bovis in degenerated neutrophils, which was confirmed by electron microscopy. The main inflammatory cell response to M. bovis infections was degenerate neutrophils, which caused the destruction of the bronchial epithelium and possibly induced epithelioid granulomatous inflammation, resulting in severe chronic respiratory diseases in cattle. Chulalongkorn University 2022-01-24 Article PeerReviewed Suwanruengsri, Mathurot and Uemura, Ryoko and Uda Zahli, Izzati and Kanda, Takuya and Fuke, Naoyuki and Yasuda, Masahiro and Hirai, Takuya and Yamaguchi, Ryoji (2022) Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves. Thai Journal of Veterinary Medicine, 52 (1). 33 - 44. ISSN 0125-6491; ESSN: 2985-1130 https://he01.tci-thaijo.org/index.php/tjvm/article/view/254775 10.14456/tjvm.2022.4
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Mycoplasma bovis (M. bovis) is a pathogen that especially effects the respiratory system of cattle. We focused on the histopathological changes in the lungs caused by M. bovis because the lesions in the bronchi or bronchioles and alveoli are quite different in natural infections. Thirty-five lung samples from 2-to 12-month-old Japanese Black calves with respiratory symptoms were collected and examined for bacteria presence and pathological changes using immunohistochemistry (IHC). Using the loop-mediated isothermal amplification method, 18 of the 35 samples were found to be positive for M. bovis, which was isolated and identified. In 7 of the samples, only M. bovis was detected, whereas in the other 11, other bacteria coexisted with M. bovis. Anti-M. bovis, anti-MAC387, anti-Iba1, anti-CD3, anti-CD20, anti-AE1/AE3 cytokeratin and anti-IL8 were used for antigen detection. Histopathological studies and diagnoses of the lungs were examined in two parts; bronchiolar lesions and alveolar lesions. The bronchiolar findings were divided into three groups: granulomatous, caseonecrotic and suppurative bronchiolitis. The alveolar lesions found were quite mild, except in three cases. IHC staining revealed strong evidence of M. bovis in degenerated neutrophils, which was confirmed by electron microscopy. The main inflammatory cell response to M. bovis infections was degenerate neutrophils, which caused the destruction of the bronchial epithelium and possibly induced epithelioid granulomatous inflammation, resulting in severe chronic respiratory diseases in cattle.
format	Article
author	Suwanruengsri, Mathurot Uemura, Ryoko Uda Zahli, Izzati Kanda, Takuya Fuke, Naoyuki Yasuda, Masahiro Hirai, Takuya Yamaguchi, Ryoji
spellingShingle	Suwanruengsri, Mathurot Uemura, Ryoko Uda Zahli, Izzati Kanda, Takuya Fuke, Naoyuki Yasuda, Masahiro Hirai, Takuya Yamaguchi, Ryoji Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
author_facet	Suwanruengsri, Mathurot Uemura, Ryoko Uda Zahli, Izzati Kanda, Takuya Fuke, Naoyuki Yasuda, Masahiro Hirai, Takuya Yamaguchi, Ryoji
author_sort	Suwanruengsri, Mathurot
title	Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
title_short	Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
title_full	Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
title_fullStr	Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
title_full_unstemmed	Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
title_sort	natural mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves
publisher	Chulalongkorn University
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/102288/ https://he01.tci-thaijo.org/index.php/tjvm/article/view/254775
_version_	1781706716456419328
score	13.211869

Natural Mycoplasma bovis infection associated with epithelioid granulomatous caseonecrotic bronchiolitis at a progressed disease stage in calves

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