Staff View: Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion

Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion

A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and se...

Full description

Saved in:

Bibliographic Details
Main Authors:	Mir, Mohammad Imran, Hameeda, Bee, Quadriya, Humera, Kumar, B. Kiran, Ilyas, Noshin, Tan, Ali Kee Zuan, El Enshasy, Hesham Ali, Dailin, Daniel Joe, Kassem, Hazem S., Abdul Gafur, Sayyed, R. Z.
Format:	Article
Published:	Frontiers Research Foundation 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/102254/ https://www.frontiersin.org/articles/10.3389/fnut.2021.781764/full
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.upm.eprints.102254
record_format	eprints
spelling	my.upm.eprints.1022542023-06-19T08:21:28Z http://psasir.upm.edu.my/id/eprint/102254/ Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion Mir, Mohammad Imran Hameeda, Bee Quadriya, Humera Kumar, B. Kiran Ilyas, Noshin Tan, Ali Kee Zuan El Enshasy, Hesham Ali Dailin, Daniel Joe Kassem, Hazem S. Abdul Gafur Sayyed, R. Z. A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C2H4/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice. Frontiers Research Foundation 2022-01-13 Article PeerReviewed Mir, Mohammad Imran and Hameeda, Bee and Quadriya, Humera and Kumar, B. Kiran and Ilyas, Noshin and Tan, Ali Kee Zuan and El Enshasy, Hesham Ali and Dailin, Daniel Joe and Kassem, Hazem S. and Abdul Gafur and Sayyed, R. Z. (2022) Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion. Frontiers in Nutrition, 8. art. no. 781764. pp. 1-14. ISSN 2296-861X https://www.frontiersin.org/articles/10.3389/fnut.2021.781764/full 10.3389/fnut.2021.781764
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C2H4/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice.
format	Article
author	Mir, Mohammad Imran Hameeda, Bee Quadriya, Humera Kumar, B. Kiran Ilyas, Noshin Tan, Ali Kee Zuan El Enshasy, Hesham Ali Dailin, Daniel Joe Kassem, Hazem S. Abdul Gafur Sayyed, R. Z.
spellingShingle	Mir, Mohammad Imran Hameeda, Bee Quadriya, Humera Kumar, B. Kiran Ilyas, Noshin Tan, Ali Kee Zuan El Enshasy, Hesham Ali Dailin, Daniel Joe Kassem, Hazem S. Abdul Gafur Sayyed, R. Z. Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
author_facet	Mir, Mohammad Imran Hameeda, Bee Quadriya, Humera Kumar, B. Kiran Ilyas, Noshin Tan, Ali Kee Zuan El Enshasy, Hesham Ali Dailin, Daniel Joe Kassem, Hazem S. Abdul Gafur Sayyed, R. Z.
author_sort	Mir, Mohammad Imran
title	Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
title_short	Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
title_full	Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
title_fullStr	Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
title_full_unstemmed	Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion
title_sort	multifarious indigenous diazotrophic rhizobacteria of rice (oryza sativa l.) rhizosphere and their effect on plant growth promotion
publisher	Frontiers Research Foundation
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/102254/ https://www.frontiersin.org/articles/10.3389/fnut.2021.781764/full
_version_	1769844439460610048
score	13.211869

Multifarious indigenous diazotrophic rhizobacteria of rice (Oryza sativa L.) Rhizosphere and their effect on plant growth promotion

Similar Items