Staff View: Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts

Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts

The aim of this study is to assess the antifungal action of Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts in altering the morphology and causing cellular damage of Fusarium oxysporum, Fusarium proliferatum, Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The...

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Main Authors:	Abd Murad, N. B., Mustafa, M., Shaari, K., Mohd Zainudin, N. A. I.
Format:	Article
Published:	Wiley-Blackwell Publishing 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/102170/ https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13801
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spelling	my.upm.eprints.1021702023-08-11T08:24:33Z http://psasir.upm.edu.my/id/eprint/102170/ Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts Abd Murad, N. B. Mustafa, M. Shaari, K. Mohd Zainudin, N. A. I. The aim of this study is to assess the antifungal action of Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts in altering the morphology and causing cellular damage of Fusarium oxysporum, Fusarium proliferatum, Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The pathogens were cultured on media containing both extracts individually and carbendazim as positive control, whereas media alone as negative control. All samples were processed for microscopy observations using scanning (SEM) and transmission electron (TEM) microscopes. Observation via SEM showed significant alterations in the hyphae of F. oxysporum, F. proliferatum and C. gloeosporioides compared to the control in which the hyphae were in normal form. However, no significant alteration in hyphae had been observed in the treated plate compared to the control for L. theobromae. The development of calcium carbonate crystals was also observed abundantly in control compared to treated pathogens for F. oxysporum and F. proliferatum only. This indicated that the plant extracts can inhibit some metabolic processes in the pathogens too. Observations via TEM had been conducted for F. proliferatum and C. gloeosporioides, respectively. The results showed disintegration of cytoplasmic organelles and cell wall, intense vacuolization and lyses part of fungal cells. The plant extracts have equivalent or even greater effects compared to commercial fungicide carbendazim. Wiley-Blackwell Publishing 2022 Article PeerReviewed Abd Murad, N. B. and Mustafa, M. and Shaari, K. and Mohd Zainudin, N. A. I. (2022) Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts. Letters in Applied Microbiology, 75 (5). 1319 -1329. ISSN 0266-8254; ESSN: 1472-765X https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13801 10.1111/lam.13801
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	The aim of this study is to assess the antifungal action of Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts in altering the morphology and causing cellular damage of Fusarium oxysporum, Fusarium proliferatum, Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The pathogens were cultured on media containing both extracts individually and carbendazim as positive control, whereas media alone as negative control. All samples were processed for microscopy observations using scanning (SEM) and transmission electron (TEM) microscopes. Observation via SEM showed significant alterations in the hyphae of F. oxysporum, F. proliferatum and C. gloeosporioides compared to the control in which the hyphae were in normal form. However, no significant alteration in hyphae had been observed in the treated plate compared to the control for L. theobromae. The development of calcium carbonate crystals was also observed abundantly in control compared to treated pathogens for F. oxysporum and F. proliferatum only. This indicated that the plant extracts can inhibit some metabolic processes in the pathogens too. Observations via TEM had been conducted for F. proliferatum and C. gloeosporioides, respectively. The results showed disintegration of cytoplasmic organelles and cell wall, intense vacuolization and lyses part of fungal cells. The plant extracts have equivalent or even greater effects compared to commercial fungicide carbendazim.
format	Article
author	Abd Murad, N. B. Mustafa, M. Shaari, K. Mohd Zainudin, N. A. I.
spellingShingle	Abd Murad, N. B. Mustafa, M. Shaari, K. Mohd Zainudin, N. A. I. Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
author_facet	Abd Murad, N. B. Mustafa, M. Shaari, K. Mohd Zainudin, N. A. I.
author_sort	Abd Murad, N. B.
title	Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
title_short	Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
title_full	Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
title_fullStr	Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
title_full_unstemmed	Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts
title_sort	micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with averrhoa bilimbi fruit and garcinia mangostana pericarp ethanolic extracts
publisher	Wiley-Blackwell Publishing
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/102170/ https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13801
_version_	1775624510981013504
score	13.211869

Micrograph analysis of morphological alteration and cellular damage of fruit rot fungal pathogens treated with Averrhoa bilimbi fruit and Garcinia mangostana pericarp ethanolic extracts

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