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Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1

This study was aimed at evaluating the influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of Lactococcus lactis Gh1 after drying and during a long-term storage for 120 days. Highest survivability rate after fre...

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Main Authors: Jawan, Roslina, Abbasiliasi, Sahar, Tan, Joo Shun, Kapri, Mohd Rizal, Mustafa, Shuhaimi, Ariff, Arbakariya
Format: Article
Published: Taylor & Francis 2022
Online Access:http://psasir.upm.edu.my/id/eprint/101913/
https://www.tandfonline.com/doi/abs/10.1080/07373937.2021.1874968
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spelling my.upm.eprints.1019132023-07-10T00:26:43Z http://psasir.upm.edu.my/id/eprint/101913/ Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1 Jawan, Roslina Abbasiliasi, Sahar Tan, Joo Shun Kapri, Mohd Rizal Mustafa, Shuhaimi Ariff, Arbakariya This study was aimed at evaluating the influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of Lactococcus lactis Gh1 after drying and during a long-term storage for 120 days. Highest survivability rate after freeze-drying was observed for cells protected in galactose followed by trehalose and peptone. The differences in cell viability among the different types of protective agent and between the concentrations after freeze-drying were not significant. However, significant difference was seen between the protective agents at different concentrations during storage. At high storage temperatures, the number of viable cells decreased for all protective agents and concentrations investigated in this study. Antibacterial activity from L. lactis Gh1 against Listeria monocytogenes ATCC 15313 was in linear correlation with the viable cell counts. Possible formulation of protective agent from this study could be applied in the industrial development of probiotic and bioprotective cultures. Taylor & Francis 2022-02-05 Article PeerReviewed Jawan, Roslina and Abbasiliasi, Sahar and Tan, Joo Shun and Kapri, Mohd Rizal and Mustafa, Shuhaimi and Ariff, Arbakariya (2022) Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1. Drying Technology, 40 (9). pp. 1774-1790. ISSN 0737-3937; ESSN: 1532-2300 https://www.tandfonline.com/doi/abs/10.1080/07373937.2021.1874968 10.1080/07373937.2021.1874968
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description This study was aimed at evaluating the influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of Lactococcus lactis Gh1 after drying and during a long-term storage for 120 days. Highest survivability rate after freeze-drying was observed for cells protected in galactose followed by trehalose and peptone. The differences in cell viability among the different types of protective agent and between the concentrations after freeze-drying were not significant. However, significant difference was seen between the protective agents at different concentrations during storage. At high storage temperatures, the number of viable cells decreased for all protective agents and concentrations investigated in this study. Antibacterial activity from L. lactis Gh1 against Listeria monocytogenes ATCC 15313 was in linear correlation with the viable cell counts. Possible formulation of protective agent from this study could be applied in the industrial development of probiotic and bioprotective cultures.
format Article
author Jawan, Roslina
Abbasiliasi, Sahar
Tan, Joo Shun
Kapri, Mohd Rizal
Mustafa, Shuhaimi
Ariff, Arbakariya
spellingShingle Jawan, Roslina
Abbasiliasi, Sahar
Tan, Joo Shun
Kapri, Mohd Rizal
Mustafa, Shuhaimi
Ariff, Arbakariya
Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
author_facet Jawan, Roslina
Abbasiliasi, Sahar
Tan, Joo Shun
Kapri, Mohd Rizal
Mustafa, Shuhaimi
Ariff, Arbakariya
author_sort Jawan, Roslina
title Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
title_short Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
title_full Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
title_fullStr Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
title_full_unstemmed Influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, Lactococcus lactis Gh1
title_sort influence of type and concentration of lyoprotectants, storage temperature and storage duration on cell viability and antibacterial activity of freeze-dried lactic acid bacterium, lactococcus lactis gh1
publisher Taylor & Francis
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/101913/
https://www.tandfonline.com/doi/abs/10.1080/07373937.2021.1874968
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score 13.211869

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