Cover Image

Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples

Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determinat...

Full description

Saved in:
Bibliographic Details
Main Authors: Semail, Nadhiratul Farihin, Abdul Keyon, Aemi Syazwani, Saad, Bahruddin, Kamaruzaman, Sazlinda, Mohamad Zain, Nur Nadhirah, Lim, Vuanghao, Miskam, Mazidatulakmam, Wan Abdullah, Wan Nazwanie, Raoov, Muggundha, Yahaya, Noorfatimah
Format: Article
Published: Elsevier 2022
Online Access:http://psasir.upm.edu.my/id/eprint/101878/
https://www.sciencedirect.com/science/article/pii/S002196732200807X
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.101878
record_format eprints
spelling my.upm.eprints.1018782023-09-08T01:36:24Z http://psasir.upm.edu.my/id/eprint/101878/ Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples Semail, Nadhiratul Farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Kamaruzaman, Sazlinda Mohamad Zain, Nur Nadhirah Lim, Vuanghao Miskam, Mazidatulakmam Wan Abdullah, Wan Nazwanie Raoov, Muggundha Yahaya, Noorfatimah Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs); ketoprofen, diclofenac and naproxen from aqueous matrices. Prior to the system setup, various parameters were optimised to assess the potential use of the t-ITP paired with the sweeping stacking technique in micellar background electrolyte for dual preconcentration and separation of trace amounts of NSAIDs. Once the optimum conditions have been established, the method performance was validated and applied to 17 environmental water samples. Based on the results, the combined t-ITP and sweeping approach significantly improved the stacking and separation sensitivity. A large volume of samples could also be introduced and subsequently separated by MEKC with greater focusing effects due to the sweeping. Under optimised conditions, the developed method exhibited excellent linearity at a high range (0.1–500 ng/mL, r2 ≥ 0.998), low limits of detection (LODs) of 0.01–0.07 ng/mL, and a remarkable relative recovery (RR) of 99.6–101.9% with a relative standard deviation (RSD) of 1.4–8.6% (n = 9). Ultimately, the sensitivity enhancement factors improved up to 666-fold using the optimised method. Therefore, the proposed method presents a simplified yet effective and suitable for the determination of NSAIDs from aqueous matrices. Elsevier 2022 Article PeerReviewed Semail, Nadhiratul Farihin and Abdul Keyon, Aemi Syazwani and Saad, Bahruddin and Kamaruzaman, Sazlinda and Mohamad Zain, Nur Nadhirah and Lim, Vuanghao and Miskam, Mazidatulakmam and Wan Abdullah, Wan Nazwanie and Raoov, Muggundha and Yahaya, Noorfatimah (2022) Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples. Journal of Chromatography A, 1685. pp. 1-10. ISSN 0021-9673; ESSN: 1873-3778 https://www.sciencedirect.com/science/article/pii/S002196732200807X 10.1016/j.chroma.2022.463616
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs); ketoprofen, diclofenac and naproxen from aqueous matrices. Prior to the system setup, various parameters were optimised to assess the potential use of the t-ITP paired with the sweeping stacking technique in micellar background electrolyte for dual preconcentration and separation of trace amounts of NSAIDs. Once the optimum conditions have been established, the method performance was validated and applied to 17 environmental water samples. Based on the results, the combined t-ITP and sweeping approach significantly improved the stacking and separation sensitivity. A large volume of samples could also be introduced and subsequently separated by MEKC with greater focusing effects due to the sweeping. Under optimised conditions, the developed method exhibited excellent linearity at a high range (0.1–500 ng/mL, r2 ≥ 0.998), low limits of detection (LODs) of 0.01–0.07 ng/mL, and a remarkable relative recovery (RR) of 99.6–101.9% with a relative standard deviation (RSD) of 1.4–8.6% (n = 9). Ultimately, the sensitivity enhancement factors improved up to 666-fold using the optimised method. Therefore, the proposed method presents a simplified yet effective and suitable for the determination of NSAIDs from aqueous matrices.
format Article
author Semail, Nadhiratul Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
spellingShingle Semail, Nadhiratul Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
author_facet Semail, Nadhiratul Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
author_sort Semail, Nadhiratul Farihin
title Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_short Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_full Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_fullStr Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_full_unstemmed Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_sort induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
publisher Elsevier
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/101878/
https://www.sciencedirect.com/science/article/pii/S002196732200807X
_version_ 1778163751063650304
score 13.211869

Similar Items