Staff View: Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro

Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro

Objectives: Hematopoietic stem cells (HSCs) reside in a specialised microenvironment in the bone marrow, which is majorly composed of mesenchymal stem cells (MSCs) and its' derivatives. This study aimed to investigate the regulatory role of MSCs to decipher the cellular and humoral communicatio...

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Main Authors:	Boroojerdi, Mohadese Hashem, Sarmadi, Vahid Hosseinpour, Maqbool, Maryam, Ling, King Hwa, Safarzadeh Kozani, Pooria, Safarzadeh Kozani, Pouya, Ramasamy, Rajesh
Format:	Article
Published:	Elsevier B.V. 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100970/ https://www.sciencedirect.com/science/article/pii/S0378111922000373
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spelling	my.upm.eprints.1009702023-07-13T07:42:17Z http://psasir.upm.edu.my/id/eprint/100970/ Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro Boroojerdi, Mohadese Hashem Sarmadi, Vahid Hosseinpour Maqbool, Maryam Ling, King Hwa Safarzadeh Kozani, Pooria Safarzadeh Kozani, Pouya Ramasamy, Rajesh Objectives: Hematopoietic stem cells (HSCs) reside in a specialised microenvironment in the bone marrow, which is majorly composed of mesenchymal stem cells (MSCs) and its' derivatives. This study aimed to investigate the regulatory role of MSCs to decipher the cellular and humoral communications on HSCs' proliferation, self-renewal, and differentiation at the transcriptomic level. Materials and Methods: Microarray assay was employed to analyse the gene expression profile of HSCs that imparted by MSCs during co-culture. Results: The proliferation of human umbilical cord blood-derived HSCs (hUC-HSCs) markedly propagated when MSCs were used as the feeder layer, without disturbing the undifferentiated state of HSCs, and reduced the cell death of HSCs. Upon co-culture with MSCs, the global microarray analysis of HSCs disclosed 712 differentially expressed genes (DEGs) (561 up-regulated and 151 down-regulated). The dysregulations of various transcripts were enriched for cellular functions such as cell cycle (including CCND1), apoptosis (including TNF), and genes related to signalling pathways governing self-renewal, as well as WNT5A from the Wnt signalling pathway, MAPK, Hedgehog, FGF2 from FGF, Jak-STAT, and PITX2 from the TGF-β signalling pathway. To concur this, real-time quantitative PCR (RT-qPCR) was utilised for corroborating the microarray results from five of the most dysregulated genes. Conclusion: This study elucidates the underlying mechanisms of the mitogenic influences of MSCs on the propagation of HSCs. The exploitation of such mechanisms provides a potential means for achieving larger quantities of HSCs in vitro, thus obviating the need for manipulating their differentiation potential for clinical application. Elsevier B.V. 2022-04-30 Article PeerReviewed Boroojerdi, Mohadese Hashem and Sarmadi, Vahid Hosseinpour and Maqbool, Maryam and Ling, King Hwa and Safarzadeh Kozani, Pooria and Safarzadeh Kozani, Pouya and Ramasamy, Rajesh (2022) Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro. Gene, 820. art. no. 146218. pp. 1-14. ISSN 0378-1119 https://www.sciencedirect.com/science/article/pii/S0378111922000373 10.1016/j.gene.2022.146218
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Objectives: Hematopoietic stem cells (HSCs) reside in a specialised microenvironment in the bone marrow, which is majorly composed of mesenchymal stem cells (MSCs) and its' derivatives. This study aimed to investigate the regulatory role of MSCs to decipher the cellular and humoral communications on HSCs' proliferation, self-renewal, and differentiation at the transcriptomic level. Materials and Methods: Microarray assay was employed to analyse the gene expression profile of HSCs that imparted by MSCs during co-culture. Results: The proliferation of human umbilical cord blood-derived HSCs (hUC-HSCs) markedly propagated when MSCs were used as the feeder layer, without disturbing the undifferentiated state of HSCs, and reduced the cell death of HSCs. Upon co-culture with MSCs, the global microarray analysis of HSCs disclosed 712 differentially expressed genes (DEGs) (561 up-regulated and 151 down-regulated). The dysregulations of various transcripts were enriched for cellular functions such as cell cycle (including CCND1), apoptosis (including TNF), and genes related to signalling pathways governing self-renewal, as well as WNT5A from the Wnt signalling pathway, MAPK, Hedgehog, FGF2 from FGF, Jak-STAT, and PITX2 from the TGF-β signalling pathway. To concur this, real-time quantitative PCR (RT-qPCR) was utilised for corroborating the microarray results from five of the most dysregulated genes. Conclusion: This study elucidates the underlying mechanisms of the mitogenic influences of MSCs on the propagation of HSCs. The exploitation of such mechanisms provides a potential means for achieving larger quantities of HSCs in vitro, thus obviating the need for manipulating their differentiation potential for clinical application.
format	Article
author	Boroojerdi, Mohadese Hashem Sarmadi, Vahid Hosseinpour Maqbool, Maryam Ling, King Hwa Safarzadeh Kozani, Pooria Safarzadeh Kozani, Pouya Ramasamy, Rajesh
spellingShingle	Boroojerdi, Mohadese Hashem Sarmadi, Vahid Hosseinpour Maqbool, Maryam Ling, King Hwa Safarzadeh Kozani, Pooria Safarzadeh Kozani, Pouya Ramasamy, Rajesh Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
author_facet	Boroojerdi, Mohadese Hashem Sarmadi, Vahid Hosseinpour Maqbool, Maryam Ling, King Hwa Safarzadeh Kozani, Pooria Safarzadeh Kozani, Pouya Ramasamy, Rajesh
author_sort	Boroojerdi, Mohadese Hashem
title	Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
title_short	Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
title_full	Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
title_fullStr	Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
title_full_unstemmed	Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
title_sort	directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro
publisher	Elsevier B.V.
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/100970/ https://www.sciencedirect.com/science/article/pii/S0378111922000373
_version_	1772813418322984960
score	13.211869

Directional capacity of human mesenchymal stem cells to support hematopoietic stem cell proliferation in vitro

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