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Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia

Pumpkin vines (Cucurbita moschata) exhibiting symptoms of shortened internodes and stunting of the vine were observed in vegetable farms in the state of Selangor, Malaysia. Nested PCR assay with 16SrRNA gene specific primers proved the presence of phytoplasma DNA in all symptomatic samples. BLAST an...

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Main Authors: Tennakoon, T. M. N. D., Naderali, N., Kong, L. L., Ahmad, K. B., Vadamalai, G.
Format: Article
Published: Springer Nature 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100914/
https://www.springer.com/journal/42360
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spelling my.upm.eprints.1009142023-07-14T08:52:37Z http://psasir.upm.edu.my/id/eprint/100914/ Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia Tennakoon, T. M. N. D. Naderali, N. Kong, L. L. Ahmad, K. B. Vadamalai, G. Pumpkin vines (Cucurbita moschata) exhibiting symptoms of shortened internodes and stunting of the vine were observed in vegetable farms in the state of Selangor, Malaysia. Nested PCR assay with 16SrRNA gene specific primers proved the presence of phytoplasma DNA in all symptomatic samples. BLAST analysis of 16SrRNA gene sequence of the pumpkin phytoplasma isolates showed 99% sequence identity with the Cucurbita pepo ‘witches’ broom phytoplasma (16SrI). In addition, virtual RFLP analysis of 16SrRNA gene sequence and the phylogenetic tree analysis confirmed that the phytoplasma detected in one symptomatic sample could belong to a new sub group of 16SrI from the previously recognized sub groups of 16SrI group by the HinfI profile whereas other phytoplasmas detected in symptomatic samples tentatively sorted as a member of 16Sr group I, subgroup B. Springer Nature 2022-07-22 Article PeerReviewed Tennakoon, T. M. N. D. and Naderali, N. and Kong, L. L. and Ahmad, K. B. and Vadamalai, G. (2022) Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia. Indian Phytopathology, 75. 1205 - 1209. ISSN 0367-973X; ESSN: 2248-9800 https://www.springer.com/journal/42360 10.1007/s42360-022-00533-5
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Pumpkin vines (Cucurbita moschata) exhibiting symptoms of shortened internodes and stunting of the vine were observed in vegetable farms in the state of Selangor, Malaysia. Nested PCR assay with 16SrRNA gene specific primers proved the presence of phytoplasma DNA in all symptomatic samples. BLAST analysis of 16SrRNA gene sequence of the pumpkin phytoplasma isolates showed 99% sequence identity with the Cucurbita pepo ‘witches’ broom phytoplasma (16SrI). In addition, virtual RFLP analysis of 16SrRNA gene sequence and the phylogenetic tree analysis confirmed that the phytoplasma detected in one symptomatic sample could belong to a new sub group of 16SrI from the previously recognized sub groups of 16SrI group by the HinfI profile whereas other phytoplasmas detected in symptomatic samples tentatively sorted as a member of 16Sr group I, subgroup B.
format Article
author Tennakoon, T. M. N. D.
Naderali, N.
Kong, L. L.
Ahmad, K. B.
Vadamalai, G.
spellingShingle Tennakoon, T. M. N. D.
Naderali, N.
Kong, L. L.
Ahmad, K. B.
Vadamalai, G.
Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
author_facet Tennakoon, T. M. N. D.
Naderali, N.
Kong, L. L.
Ahmad, K. B.
Vadamalai, G.
author_sort Tennakoon, T. M. N. D.
title Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
title_short Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
title_full Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
title_fullStr Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
title_full_unstemmed Detection and identification of 16SrI-B and a novel 16SrI phytoplasma subgroups associated with leaf malformation of Cucurbita moschata (pumpkin) in Malaysia
title_sort detection and identification of 16sri-b and a novel 16sri phytoplasma subgroups associated with leaf malformation of cucurbita moschata (pumpkin) in malaysia
publisher Springer Nature
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/100914/
https://www.springer.com/journal/42360
_version_ 1772813408696008704
score 13.211869

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