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Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens

Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-d...

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Main Authors: Yaacob, Jamilah Syafawati, Ramli, Muhammad Aiman, Abd Rahim, Muhamad Hafiz, Selvaraj, Abigail Marie Robert, Nyanasaigran, Laavanya
Format: Article
Published: Sains Malaysiana 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100736/
https://www.ukm.my/jsm/english_journals/vol51num10_2022/vol51num10_2022pg3171-3182.html
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spelling my.upm.eprints.1007362023-09-11T08:01:00Z http://psasir.upm.edu.my/id/eprint/100736/ Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens Yaacob, Jamilah Syafawati Ramli, Muhammad Aiman Abd Rahim, Muhamad Hafiz Selvaraj, Abigail Marie Robert Nyanasaigran, Laavanya Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-dichlorophenoxyacetic acid (2,4-D) on the expression of compounds in coloured callus of Capsicum frutescens, a vital spice in various cuisines worldwide. The differential accumulation of compounds in the callus was analysed using liquid chromatography-mass spectrometry (LCMS) and the PGR concentration that resulted in the highest accumulation of the valuable compounds was identified. In this study, calli of various colours (cream, yellow and green) were successfully produced from C. frutescens through plant tissue culture. The increase in 2,4-D concentrations was found to increase callus growth index (GI) and specific growth rate (Sg), where the highest GI (0.5690) and Sg (0.6348 mg/week) were observed in callus produced in media supplemented with 0.5 mg/L 2,4-D. LCMS data analyses showed that 19 compounds were detected in the callus, with 8 compounds (fatty acids and phenolics) were successfully identified, while the remaining 11 compounds were reported as unknowns. Yellow-coloured callus was observed to contain the highest number of compounds (18 compounds), while green callus contained the least (14 compounds). This analysis provides valuable information on the application of biotechnological tools such as plant tissue culture as an alternative for sustainable production of compounds with high bioactivity in Capsicum frutescens. Sains Malaysiana 2022-10 Article PeerReviewed Yaacob, Jamilah Syafawati and Ramli, Muhammad Aiman and Abd Rahim, Muhamad Hafiz and Selvaraj, Abigail Marie Robert and Nyanasaigran, Laavanya (2022) Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens. Penerbit Universiti Kebangsaan Malaysia, 51 (10). 3171 - 3182. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol51num10_2022/vol51num10_2022pg3171-3182.html 10.17576/jsm-2022-5110-05
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-dichlorophenoxyacetic acid (2,4-D) on the expression of compounds in coloured callus of Capsicum frutescens, a vital spice in various cuisines worldwide. The differential accumulation of compounds in the callus was analysed using liquid chromatography-mass spectrometry (LCMS) and the PGR concentration that resulted in the highest accumulation of the valuable compounds was identified. In this study, calli of various colours (cream, yellow and green) were successfully produced from C. frutescens through plant tissue culture. The increase in 2,4-D concentrations was found to increase callus growth index (GI) and specific growth rate (Sg), where the highest GI (0.5690) and Sg (0.6348 mg/week) were observed in callus produced in media supplemented with 0.5 mg/L 2,4-D. LCMS data analyses showed that 19 compounds were detected in the callus, with 8 compounds (fatty acids and phenolics) were successfully identified, while the remaining 11 compounds were reported as unknowns. Yellow-coloured callus was observed to contain the highest number of compounds (18 compounds), while green callus contained the least (14 compounds). This analysis provides valuable information on the application of biotechnological tools such as plant tissue culture as an alternative for sustainable production of compounds with high bioactivity in Capsicum frutescens.
format Article
author Yaacob, Jamilah Syafawati
Ramli, Muhammad Aiman
Abd Rahim, Muhamad Hafiz
Selvaraj, Abigail Marie Robert
Nyanasaigran, Laavanya
spellingShingle Yaacob, Jamilah Syafawati
Ramli, Muhammad Aiman
Abd Rahim, Muhamad Hafiz
Selvaraj, Abigail Marie Robert
Nyanasaigran, Laavanya
Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
author_facet Yaacob, Jamilah Syafawati
Ramli, Muhammad Aiman
Abd Rahim, Muhamad Hafiz
Selvaraj, Abigail Marie Robert
Nyanasaigran, Laavanya
author_sort Yaacob, Jamilah Syafawati
title Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
title_short Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
title_full Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
title_fullStr Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
title_full_unstemmed Comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of Capsicum frutescens
title_sort comparative analysis on the role of 2,4-dichlorophenoxyacetic acid in the expression of bioactive compounds in callus of capsicum frutescens
publisher Sains Malaysiana
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/100736/
https://www.ukm.my/jsm/english_journals/vol51num10_2022/vol51num10_2022pg3171-3182.html
_version_ 1778163736849154048
score 13.211869

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