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Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste

Current chitosan extraction methods require a highly concentrated alkaline solvent that is costly and environmentally unfriendly. This study aimed to assess the potential of using subcritical liquid deproteination to produce amorphous chitin to reduce the alkaline concentration during the deacetylat...

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Main Authors: Segaran, Ramyah, Omar, Rozita, Kusumastuti, Yuni, Harun, Razif, Mustafa Kamal, Siti Mazlina
Format: Article
Published: Polish Chitin Society 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100722/
http://ptchit.lodz.pl/en/2022/10/21/xxvii-2022/
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spelling my.upm.eprints.1007222023-09-11T06:55:58Z http://psasir.upm.edu.my/id/eprint/100722/ Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste Segaran, Ramyah Omar, Rozita Kusumastuti, Yuni Harun, Razif Mustafa Kamal, Siti Mazlina Current chitosan extraction methods require a highly concentrated alkaline solvent that is costly and environmentally unfriendly. This study aimed to assess the potential of using subcritical liquid deproteination to produce amorphous chitin to reduce the alkaline concentration during the deacetylation step to obtain high-quality chitosan. A combination of microwave-assisted demineralisation, subcritical liquid (SL) deproteination, and conventional deacetylation was employed for chitosan production. Distilled water and 2% and 4% sodium hydroxide (NaOH) were used at subcritical liquid treatment temperatures of 100 to 250°C. Meanwhile, deacetylation using 20%-50% NaOH was attempted on the deproteinised chitin. 1.8 M hydrochloric acid at a 1:10 solid-to-liquid solvent ratio produced chitin with acceptable quality during microwave-assisted demineralisation. Demineralised chitin subjected to SL treatment at 150°C and 4% NaOH had the best protein removal (84.6%). Chitosan with a high degree of deacetylation (80.68%) was obtained at a lower alkalinity of 30% NaOH, derived from SL-deproteinised chitin (4% NaOH at 100°C). The crystallinity of chitin after SL treatment was reduced significantly from 72.4% to 59.4%, which allows easier access for the solvent to hydrolyse the acetamide bond. This study confirms that good quality chitosan can be produced by utilising SL treatment of demineralised chitin at a significantly short time and lower deacetylation solvent concentration. Polish Chitin Society 2022-07 Article PeerReviewed Segaran, Ramyah and Omar, Rozita and Kusumastuti, Yuni and Harun, Razif and Mustafa Kamal, Siti Mazlina (2022) Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste. Progress on Chemistry and Application of Chitin and its Derivatives (PCACD), 27. 204 - 216. ISSN 1896-5644 http://ptchit.lodz.pl/en/2022/10/21/xxvii-2022/ 10.15259/PCACD.27.016
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Current chitosan extraction methods require a highly concentrated alkaline solvent that is costly and environmentally unfriendly. This study aimed to assess the potential of using subcritical liquid deproteination to produce amorphous chitin to reduce the alkaline concentration during the deacetylation step to obtain high-quality chitosan. A combination of microwave-assisted demineralisation, subcritical liquid (SL) deproteination, and conventional deacetylation was employed for chitosan production. Distilled water and 2% and 4% sodium hydroxide (NaOH) were used at subcritical liquid treatment temperatures of 100 to 250°C. Meanwhile, deacetylation using 20%-50% NaOH was attempted on the deproteinised chitin. 1.8 M hydrochloric acid at a 1:10 solid-to-liquid solvent ratio produced chitin with acceptable quality during microwave-assisted demineralisation. Demineralised chitin subjected to SL treatment at 150°C and 4% NaOH had the best protein removal (84.6%). Chitosan with a high degree of deacetylation (80.68%) was obtained at a lower alkalinity of 30% NaOH, derived from SL-deproteinised chitin (4% NaOH at 100°C). The crystallinity of chitin after SL treatment was reduced significantly from 72.4% to 59.4%, which allows easier access for the solvent to hydrolyse the acetamide bond. This study confirms that good quality chitosan can be produced by utilising SL treatment of demineralised chitin at a significantly short time and lower deacetylation solvent concentration.
format Article
author Segaran, Ramyah
Omar, Rozita
Kusumastuti, Yuni
Harun, Razif
Mustafa Kamal, Siti Mazlina
spellingShingle Segaran, Ramyah
Omar, Rozita
Kusumastuti, Yuni
Harun, Razif
Mustafa Kamal, Siti Mazlina
Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
author_facet Segaran, Ramyah
Omar, Rozita
Kusumastuti, Yuni
Harun, Razif
Mustafa Kamal, Siti Mazlina
author_sort Segaran, Ramyah
title Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
title_short Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
title_full Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
title_fullStr Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
title_full_unstemmed Combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
title_sort combined microwave-assisted subcritical liquid extraction of chitosan from crab shell waste
publisher Polish Chitin Society
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/100722/
http://ptchit.lodz.pl/en/2022/10/21/xxvii-2022/
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score 13.211869

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