Staff View: Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin

Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin

Callogenesis and embryogenesis are integral parts of many tissue culture procedures for genetic manipulation in rice. However, the efficiency of both processes is largely dependent on the media constituent especially the plant growth regulators (PGRs) due to the genotype-dependent nature of in vitro...

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Main Authors:	Sidek, Noorhazira, Nulit, Rosimah, Yap, Chee Kong, Yong, Christina Seok Yien, Sekeli, Rogayah, EL-Barghathi, Mariam F.
Format:	Article
Published:	AIMS Press 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100578/ https://www.aimspress.com/article/doi/10.3934/agrfood.2022033?viewType=HTML
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spelling	my.upm.eprints.1005782023-11-21T08:09:37Z http://psasir.upm.edu.my/id/eprint/100578/ Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin Sidek, Noorhazira Nulit, Rosimah Yap, Chee Kong Yong, Christina Seok Yien Sekeli, Rogayah EL-Barghathi, Mariam F. Callogenesis and embryogenesis are integral parts of many tissue culture procedures for genetic manipulation in rice. However, the efficiency of both processes is largely dependent on the media constituent especially the plant growth regulators (PGRs) due to the genotype-dependent nature of in vitro culture protocols. Therefore, this study investigates the effect of two PGRs; 2, 4-dichlorophenoxyacetic acid (2, 4-D) and kinetin (Kin) on callus growth and somatic embryogenesis of an important Malaysian rice cultivar (Oryza sativa L. cv. MARDI Siraj 297). Mature rice seeds explants were inoculated in Murashige & Skoog (MS) medium supplemented with different combinations of 2, 4-D (0 to 3.5 mg/L) and Kin (0 to 0.5 mg/L) to induce callogenesis. Parameters for callus growth such as fresh weight (FW), callus induction frequency (CIF), embryogenic callus frequency (ECF), regeneration frequency (RF), number of plantlets per callus (PPC), callus texture and callus color were observed after 35 days of inoculation. The results show that the maximum callus growth was achieved in MS medium supplemented with combination of 2.0 mg/L 2, 4-D and 0.2 mg/L Kin, represented by the highest FW (211 mg), CIF (95%), ECF (90%), RF (100%) and PPC (22 plantlets); along with friable callus texture. Low concentration of 2, 4-D (0 to 0.5 mg/L) in the presence or absence of Kin promotes root growth instead of callus, while high concentrations (above 3.0 mg/L) retard the callus formation. The embryogenic calli from this optimized PGRs combination were successfully formed shoots in MS medium supplemented with 2 mg/L BAP and 1 mg/L NAA, followed by rooting in PGRs-free MS medium. This finding provides an efficient protocol for callogenesis and somatic embryogenesis of MARDI Siraj 297, since this is the first published report regarding somatic embryogenesis induction of this cultivar. AIMS Press 2022-07-14 Article PeerReviewed Sidek, Noorhazira and Nulit, Rosimah and Yap, Chee Kong and Yong, Christina Seok Yien and Sekeli, Rogayah and EL-Barghathi, Mariam F. (2022) Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin. AIMS Agriculture and Food, 7 (3). 536 - 552. ISSN 2471-2086 https://www.aimspress.com/article/doi/10.3934/agrfood.2022033?viewType=HTML 10.3934/agrfood.2022033
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Callogenesis and embryogenesis are integral parts of many tissue culture procedures for genetic manipulation in rice. However, the efficiency of both processes is largely dependent on the media constituent especially the plant growth regulators (PGRs) due to the genotype-dependent nature of in vitro culture protocols. Therefore, this study investigates the effect of two PGRs; 2, 4-dichlorophenoxyacetic acid (2, 4-D) and kinetin (Kin) on callus growth and somatic embryogenesis of an important Malaysian rice cultivar (Oryza sativa L. cv. MARDI Siraj 297). Mature rice seeds explants were inoculated in Murashige & Skoog (MS) medium supplemented with different combinations of 2, 4-D (0 to 3.5 mg/L) and Kin (0 to 0.5 mg/L) to induce callogenesis. Parameters for callus growth such as fresh weight (FW), callus induction frequency (CIF), embryogenic callus frequency (ECF), regeneration frequency (RF), number of plantlets per callus (PPC), callus texture and callus color were observed after 35 days of inoculation. The results show that the maximum callus growth was achieved in MS medium supplemented with combination of 2.0 mg/L 2, 4-D and 0.2 mg/L Kin, represented by the highest FW (211 mg), CIF (95%), ECF (90%), RF (100%) and PPC (22 plantlets); along with friable callus texture. Low concentration of 2, 4-D (0 to 0.5 mg/L) in the presence or absence of Kin promotes root growth instead of callus, while high concentrations (above 3.0 mg/L) retard the callus formation. The embryogenic calli from this optimized PGRs combination were successfully formed shoots in MS medium supplemented with 2 mg/L BAP and 1 mg/L NAA, followed by rooting in PGRs-free MS medium. This finding provides an efficient protocol for callogenesis and somatic embryogenesis of MARDI Siraj 297, since this is the first published report regarding somatic embryogenesis induction of this cultivar.
format	Article
author	Sidek, Noorhazira Nulit, Rosimah Yap, Chee Kong Yong, Christina Seok Yien Sekeli, Rogayah EL-Barghathi, Mariam F.
spellingShingle	Sidek, Noorhazira Nulit, Rosimah Yap, Chee Kong Yong, Christina Seok Yien Sekeli, Rogayah EL-Barghathi, Mariam F. Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
author_facet	Sidek, Noorhazira Nulit, Rosimah Yap, Chee Kong Yong, Christina Seok Yien Sekeli, Rogayah EL-Barghathi, Mariam F.
author_sort	Sidek, Noorhazira
title	Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
title_short	Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
title_full	Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
title_fullStr	Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
title_full_unstemmed	Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
title_sort	callogenesis and somatic embryogenesis of oryza sativa l. (cv. mardi siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin
publisher	AIMS Press
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/100578/ https://www.aimspress.com/article/doi/10.3934/agrfood.2022033?viewType=HTML
_version_	1783879934789287936
score	13.222552

Callogenesis and somatic embryogenesis of Oryza sativa L. (cv. MARDI Siraj 297) under the influence of 2, 4-dichlorophenoxyacetic acid and kinetin

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