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Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia

Introduction: Heavy metals exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals concentration give an insight on the total internal body burden. The level of heavy metals of an individual are interrelated to the genetic polymorp...

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Main Authors: Mohd Noor, Farrah Atiqah, Mohd Elias, Saliza, Abu Bakar, Suhaili, Aris, Ahmad Zaharin, Md Zulkifli, Hamizah
Format: Article
Language:English
Published: UPM Press 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100556/1/Blood%20heavy%20metals%20%28arsenic%2C%20cadmium%20and%20lead%29%20concentration%20and%20genetic%20polymorphism.pdf
http://psasir.upm.edu.my/id/eprint/100556/
https://medic.upm.edu.my/jurnal_kami/volume_18_2022/mjmhs_vol18_supp_5_april_2022-66369
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spelling my.upm.eprints.1005562023-10-10T01:45:47Z http://psasir.upm.edu.my/id/eprint/100556/ Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia Mohd Noor, Farrah Atiqah Mohd Elias, Saliza Abu Bakar, Suhaili Aris, Ahmad Zaharin Md Zulkifli, Hamizah Introduction: Heavy metals exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals concentration give an insight on the total internal body burden. The level of heavy metals of an individual are interrelated to the genetic polymorphism of glutathione S-tranferases (GSTs). This study aims to determine the association between blood arsenic (BAs), blood cadmium (BCd) and blood lead (BPb) concentration as reported in previous report and polymorphic variants of GST genes. Methods: Sixty three adult respondents were recruited along the coastal area of Melaka through purposive sampling in February 2016. Blood samples were collected through venipuncture procedure by certified personnel in EDTA tubes. The inductively coupled plasma- mass spectrometry (ICP-MS) instrumentation was used to analyse the blood heavy metals concentration. DNA was extracted from the whole blood samples. The polymorphic variants of GSTM1 and GSTT1 were analysed by multiplex polymerase chain reaction (PCR) and polymorphic variants of GSTP1 were analysed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Results: The BAs, BCd and BPb median (IQR) concentrations detected were 0.076 (0.059 – 0.107), 1.204 (0.670 – 2.094) and 0.076 (0.038-0.138) (µg/L) respectively. The prevalence of genetic polymorphism in GSTM1 and GSTT1 of 63.5% and 38.1% were observed. The number of respondents who have both deleted genes for GSTM1 and GSTT1 was 17 (27%). The frequency of genetic polymorphism in GSTP1 was 55.5%, in which 29 (46.0%) of the respondents were having the heterozygous (Ile/Val) polymorphism while the other 6 (9.5%) respondents were having the homozygous mutant (Val/Val). Conclusion: The polymorphic GST gene variations were not statistically linked to heavy metal concentrations. Respondents with the Val/Val genotype in GSTP1 and both GSTM1 and GSTT1 deletions, had a greater median of BAs and BPb UPM Press 2022-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/100556/1/Blood%20heavy%20metals%20%28arsenic%2C%20cadmium%20and%20lead%29%20concentration%20and%20genetic%20polymorphism.pdf Mohd Noor, Farrah Atiqah and Mohd Elias, Saliza and Abu Bakar, Suhaili and Aris, Ahmad Zaharin and Md Zulkifli, Hamizah (2022) Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia. Malaysian Journal of Medicine and Health Sciences, 18 (suppl. 5). art. no. 6. 45 - 52. ISSN 2636-9346 https://medic.upm.edu.my/jurnal_kami/volume_18_2022/mjmhs_vol18_supp_5_april_2022-66369
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Heavy metals exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals concentration give an insight on the total internal body burden. The level of heavy metals of an individual are interrelated to the genetic polymorphism of glutathione S-tranferases (GSTs). This study aims to determine the association between blood arsenic (BAs), blood cadmium (BCd) and blood lead (BPb) concentration as reported in previous report and polymorphic variants of GST genes. Methods: Sixty three adult respondents were recruited along the coastal area of Melaka through purposive sampling in February 2016. Blood samples were collected through venipuncture procedure by certified personnel in EDTA tubes. The inductively coupled plasma- mass spectrometry (ICP-MS) instrumentation was used to analyse the blood heavy metals concentration. DNA was extracted from the whole blood samples. The polymorphic variants of GSTM1 and GSTT1 were analysed by multiplex polymerase chain reaction (PCR) and polymorphic variants of GSTP1 were analysed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Results: The BAs, BCd and BPb median (IQR) concentrations detected were 0.076 (0.059 – 0.107), 1.204 (0.670 – 2.094) and 0.076 (0.038-0.138) (µg/L) respectively. The prevalence of genetic polymorphism in GSTM1 and GSTT1 of 63.5% and 38.1% were observed. The number of respondents who have both deleted genes for GSTM1 and GSTT1 was 17 (27%). The frequency of genetic polymorphism in GSTP1 was 55.5%, in which 29 (46.0%) of the respondents were having the heterozygous (Ile/Val) polymorphism while the other 6 (9.5%) respondents were having the homozygous mutant (Val/Val). Conclusion: The polymorphic GST gene variations were not statistically linked to heavy metal concentrations. Respondents with the Val/Val genotype in GSTP1 and both GSTM1 and GSTT1 deletions, had a greater median of BAs and BPb
format Article
author Mohd Noor, Farrah Atiqah
Mohd Elias, Saliza
Abu Bakar, Suhaili
Aris, Ahmad Zaharin
Md Zulkifli, Hamizah
spellingShingle Mohd Noor, Farrah Atiqah
Mohd Elias, Saliza
Abu Bakar, Suhaili
Aris, Ahmad Zaharin
Md Zulkifli, Hamizah
Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
author_facet Mohd Noor, Farrah Atiqah
Mohd Elias, Saliza
Abu Bakar, Suhaili
Aris, Ahmad Zaharin
Md Zulkifli, Hamizah
author_sort Mohd Noor, Farrah Atiqah
title Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
title_short Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
title_full Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
title_fullStr Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
title_full_unstemmed Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
title_sort blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione s-transferase genes among adults in coastal area of melaka, malaysia
publisher UPM Press
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/100556/1/Blood%20heavy%20metals%20%28arsenic%2C%20cadmium%20and%20lead%29%20concentration%20and%20genetic%20polymorphism.pdf
http://psasir.upm.edu.my/id/eprint/100556/
https://medic.upm.edu.my/jurnal_kami/volume_18_2022/mjmhs_vol18_supp_5_april_2022-66369
_version_ 1781706660048273408
score 13.211869

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