Staff View: Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro

Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro

Aspergillus section Flavi constitutes several species of opportunistic fungi, notable among them are A. flavus and A. parasiticus, capable of surviving harsh conditions and colonizing a wide range of agricultural products pre- and postharvest. Physical and chemical control methods are widely applied...

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Main Authors:	Rahman, Mohd Azuar Hamizan, Selamat, Jinap, Samsudin, Nik Iskandar Putra, Shaari, Khozirah, Mahror, Norlia, John, Joshua Mark
Format:	Article
Published:	John Wiley & Sons 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100274/ https://onlinelibrary.wiley.com/doi/full/10.1002/fsn3.2995
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spelling	my.upm.eprints.1002742024-02-14T03:45:23Z http://psasir.upm.edu.my/id/eprint/100274/ Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro Rahman, Mohd Azuar Hamizan Selamat, Jinap Samsudin, Nik Iskandar Putra Shaari, Khozirah Mahror, Norlia John, Joshua Mark Aspergillus section Flavi constitutes several species of opportunistic fungi, notable among them are A. flavus and A. parasiticus, capable of surviving harsh conditions and colonizing a wide range of agricultural products pre- and postharvest. Physical and chemical control methods are widely applied in order to mitigate the invasion of A. flavus in crops. However, physical control is not suitable for large scale and chemical control often leads to environmental pollution, whereas biological control offers a safer, environmentally friendly, and economical alternative. The present study aimed to investigate the antagonism of several non-aflatoxigenic A. flavus strains against the aflatoxigenic ones in vitro (semisynthetic peanut growth medium; MPA) in terms of colony growth rate and AFB1 inhibition. Different peanut concentrations were used to obtain the optimum peanut concentration in the formulated growth medium. A dual culture assay was performed to assess the antagonism of nonaflatoxigenic strains against the aflatoxigenic ones. Results revealed that 9% MPA exhibited the highest growth and AFB1 inhibition by nonaflatoxigenic strains. It was also found that different nonaflatoxigenic strains exhibited different antagonism against the aflatoxigenic ones which ranged from 11.09 ± 0.65% to 14.06 ± 0.14% for growth inhibition, and 53.97 ± 2.46% to 72.64 ± 4.54% for AFB1 inhibition. This variability could be due to the difference in antagonistic metabolites produced by different nonaflatoxigenic strains assessed in the present study. Metabolomics study to ascertain the specific metabolites that conferred the growth and aflatoxin inhibition is ongoing. John Wiley & Sons 2022-11 Article PeerReviewed Rahman, Mohd Azuar Hamizan and Selamat, Jinap and Samsudin, Nik Iskandar Putra and Shaari, Khozirah and Mahror, Norlia and John, Joshua Mark (2022) Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro. Food Science & Nutrition, 10 (11). 3993 - 4002. ISSN 2048-7177; ESSN: 2048-7177 https://onlinelibrary.wiley.com/doi/full/10.1002/fsn3.2995 10.1002/fsn3.2995
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Aspergillus section Flavi constitutes several species of opportunistic fungi, notable among them are A. flavus and A. parasiticus, capable of surviving harsh conditions and colonizing a wide range of agricultural products pre- and postharvest. Physical and chemical control methods are widely applied in order to mitigate the invasion of A. flavus in crops. However, physical control is not suitable for large scale and chemical control often leads to environmental pollution, whereas biological control offers a safer, environmentally friendly, and economical alternative. The present study aimed to investigate the antagonism of several non-aflatoxigenic A. flavus strains against the aflatoxigenic ones in vitro (semisynthetic peanut growth medium; MPA) in terms of colony growth rate and AFB1 inhibition. Different peanut concentrations were used to obtain the optimum peanut concentration in the formulated growth medium. A dual culture assay was performed to assess the antagonism of nonaflatoxigenic strains against the aflatoxigenic ones. Results revealed that 9% MPA exhibited the highest growth and AFB1 inhibition by nonaflatoxigenic strains. It was also found that different nonaflatoxigenic strains exhibited different antagonism against the aflatoxigenic ones which ranged from 11.09 ± 0.65% to 14.06 ± 0.14% for growth inhibition, and 53.97 ± 2.46% to 72.64 ± 4.54% for AFB1 inhibition. This variability could be due to the difference in antagonistic metabolites produced by different nonaflatoxigenic strains assessed in the present study. Metabolomics study to ascertain the specific metabolites that conferred the growth and aflatoxin inhibition is ongoing.
format	Article
author	Rahman, Mohd Azuar Hamizan Selamat, Jinap Samsudin, Nik Iskandar Putra Shaari, Khozirah Mahror, Norlia John, Joshua Mark
spellingShingle	Rahman, Mohd Azuar Hamizan Selamat, Jinap Samsudin, Nik Iskandar Putra Shaari, Khozirah Mahror, Norlia John, Joshua Mark Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
author_facet	Rahman, Mohd Azuar Hamizan Selamat, Jinap Samsudin, Nik Iskandar Putra Shaari, Khozirah Mahror, Norlia John, Joshua Mark
author_sort	Rahman, Mohd Azuar Hamizan
title	Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
title_short	Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
title_full	Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
title_fullStr	Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
title_full_unstemmed	Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro
title_sort	antagonism of nonaflatoxigenic aspergillus flavus isolated from peanuts against aflatoxigenic a. flavus growth and aflatoxin b1 production in vitro
publisher	John Wiley & Sons
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/100274/ https://onlinelibrary.wiley.com/doi/full/10.1002/fsn3.2995
_version_	1792154405874696192
score	13.211869

Antagonism of nonaflatoxigenic Aspergillus flavus isolated from peanuts against aflatoxigenic A. flavus growth and aflatoxin B1 production in vitro

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