Staff View: A novel integrative methodology for research on pot-honey variations during post-harvest

A novel integrative methodology for research on pot-honey variations during post-harvest

This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and...

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Main Authors:	Vit, Patricia, Chuttong, Bajaree, Zawawi, Norhasnida, Diaz, Maria, Meulen, Jane van der, Ahmad, Hajar F., Tomas-Barberan, Francisco A., Meccia, Gina, Danmek, Khanchai, Moreno, Jorge Enrique, Roubik, David W., Barth, Ortrud Monika, Lachenmeier, Dirk W., Engel, Michael S.
Format:	Article
Published:	Universidade Estadual de Feira de Santana 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100266/ https://periodicos.uefs.br/index.php/sociobiology/article/view/8251
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id	my.upm.eprints.100266
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spelling	my.upm.eprints.1002662024-07-09T03:44:36Z http://psasir.upm.edu.my/id/eprint/100266/ A novel integrative methodology for research on pot-honey variations during post-harvest Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. Universidade Estadual de Feira de Santana 2022-11-28 Article PeerReviewed Vit, Patricia and Chuttong, Bajaree and Zawawi, Norhasnida and Diaz, Maria and Meulen, Jane van der and Ahmad, Hajar F. and Tomas-Barberan, Francisco A. and Meccia, Gina and Danmek, Khanchai and Moreno, Jorge Enrique and Roubik, David W. and Barth, Ortrud Monika and Lachenmeier, Dirk W. and Engel, Michael S. (2022) A novel integrative methodology for research on pot-honey variations during post-harvest. Sociobiology: An international journal on social insects, 69 (4). art. no. 8251. pp. 1-27. ISSN 0361-6525 https://periodicos.uefs.br/index.php/sociobiology/article/view/8251 10.13102/sociobiology.v69i4.8251
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations.
format	Article
author	Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S.
spellingShingle	Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. A novel integrative methodology for research on pot-honey variations during post-harvest
author_facet	Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S.
author_sort	Vit, Patricia
title	A novel integrative methodology for research on pot-honey variations during post-harvest
title_short	A novel integrative methodology for research on pot-honey variations during post-harvest
title_full	A novel integrative methodology for research on pot-honey variations during post-harvest
title_fullStr	A novel integrative methodology for research on pot-honey variations during post-harvest
title_full_unstemmed	A novel integrative methodology for research on pot-honey variations during post-harvest
title_sort	novel integrative methodology for research on pot-honey variations during post-harvest
publisher	Universidade Estadual de Feira de Santana
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/100266/ https://periodicos.uefs.br/index.php/sociobiology/article/view/8251
_version_	1805889517499973632
score	13.211869

A novel integrative methodology for research on pot-honey variations during post-harvest

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