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Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application

Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process to select and identify suitable aptamers that...

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Main Authors: Gan, Zixuen, Mohamad Roslan, Muhamad Aidilfitri, Abd Shukor, Mohd Yunus, Halim, Murni, Yasid, Nur Adeela, Abdullah, Jaafar, Md Yasin, Ina Salwany, Wasoh, Helmi
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Published: Multidisciplinary Digital Publishing Institute 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100138/
https://www.mdpi.com/2079-6374/12/11/922
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spelling my.upm.eprints.1001382024-07-17T02:47:27Z http://psasir.upm.edu.my/id/eprint/100138/ Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application Gan, Zixuen Mohamad Roslan, Muhamad Aidilfitri Abd Shukor, Mohd Yunus Halim, Murni Yasid, Nur Adeela Abdullah, Jaafar Md Yasin, Ina Salwany Wasoh, Helmi Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process to select and identify suitable aptamers that show high affinity and specificity towards target cells. Great strides have been achieved in the design, construction, and use of aptamers up to this point. However, only a small number of aptamer-based applications have achieved widespread commercial and clinical acceptance. Additionally, finding more effective ways to acquire aptamers with high affinity remains a challenge. Therefore, it is crucial to thoroughly examine the existing dearth and advancement in aptamer-related technologies. This review focuses on aptamers that are generated by SELEX to detect pathogenic microorganisms and mammalian cells, as well as in cell-internalizing SELEX for diagnostic and therapeutic purposes. The development of novel aptamer-based biosensors using optical and electrical methods for microbial detection is reported. The applications and limitations of aptamers are also discussed. Multidisciplinary Digital Publishing Institute 2022-10-25 Article PeerReviewed Gan, Zixuen and Mohamad Roslan, Muhamad Aidilfitri and Abd Shukor, Mohd Yunus and Halim, Murni and Yasid, Nur Adeela and Abdullah, Jaafar and Md Yasin, Ina Salwany and Wasoh, Helmi (2022) Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application. Biosensors, 12 (11). art. no. 922. pp. 1-17. ISSN 2079-6374 https://www.mdpi.com/2079-6374/12/11/922 10.3390/bios12110922
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process to select and identify suitable aptamers that show high affinity and specificity towards target cells. Great strides have been achieved in the design, construction, and use of aptamers up to this point. However, only a small number of aptamer-based applications have achieved widespread commercial and clinical acceptance. Additionally, finding more effective ways to acquire aptamers with high affinity remains a challenge. Therefore, it is crucial to thoroughly examine the existing dearth and advancement in aptamer-related technologies. This review focuses on aptamers that are generated by SELEX to detect pathogenic microorganisms and mammalian cells, as well as in cell-internalizing SELEX for diagnostic and therapeutic purposes. The development of novel aptamer-based biosensors using optical and electrical methods for microbial detection is reported. The applications and limitations of aptamers are also discussed.
format Article
author Gan, Zixuen
Mohamad Roslan, Muhamad Aidilfitri
Abd Shukor, Mohd Yunus
Halim, Murni
Yasid, Nur Adeela
Abdullah, Jaafar
Md Yasin, Ina Salwany
Wasoh, Helmi
spellingShingle Gan, Zixuen
Mohamad Roslan, Muhamad Aidilfitri
Abd Shukor, Mohd Yunus
Halim, Murni
Yasid, Nur Adeela
Abdullah, Jaafar
Md Yasin, Ina Salwany
Wasoh, Helmi
Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
author_facet Gan, Zixuen
Mohamad Roslan, Muhamad Aidilfitri
Abd Shukor, Mohd Yunus
Halim, Murni
Yasid, Nur Adeela
Abdullah, Jaafar
Md Yasin, Ina Salwany
Wasoh, Helmi
author_sort Gan, Zixuen
title Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
title_short Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
title_full Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
title_fullStr Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
title_full_unstemmed Advances in aptamer-based biosensors and cell-internalizing SELEX technology for diagnostic and therapeutic application
title_sort advances in aptamer-based biosensors and cell-internalizing selex technology for diagnostic and therapeutic application
publisher Multidisciplinary Digital Publishing Institute
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/100138/
https://www.mdpi.com/2079-6374/12/11/922
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score 13.211869

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