Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli
Crude glycerol, an inevitable byproduct during biodiesel production, is emerging as a potential feedstock for fermentation, due to its availability and a reasonable price. The anaerobic digestion of glycerol derived from biodiesel manufacturing, in which COD was found to be 1010 g/kg, was studied in...
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my.uniten.dspace-60272018-02-21T01:03:03Z Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli Nomanbhay, S.M. Hussain, R. Crude glycerol, an inevitable byproduct during biodiesel production, is emerging as a potential feedstock for fermentation, due to its availability and a reasonable price. The anaerobic digestion of glycerol derived from biodiesel manufacturing, in which COD was found to be 1010 g/kg, was studied in batch laboratory-scale reactors. Dissimilation of glycerol by Escherichia coli is strictly linked to their capacity to synthesize the highly reduced product 1,3-propanediol (1,3-PDO). The present study is focus on the development of adaptive mutant strains of Escherichia coli EC-MW (ATCC 11105), through microwave irradiation, at frequency 2.45 GHz and irradiation time 5 min pulse irradiation. The mutants were used for high bioethanol production from glycerol feedstock. Consequently, glycerol oxidative pathway (bioethanol) enhanced upon the parallel reduction in the 1,3-propanediol (1,3-PDO) pathway. The modified E. coli strains were able to increase bioethanol production upon fermentation reaching the level 280 g/L. 2017-12-08T08:12:10Z 2017-12-08T08:12:10Z 2014 Article https://pure.uniten.edu.my/en/publications/efficient-production-of-bioethanol-from-waste-glycerol-using-micr en_US Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(5), 210-222 |
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Crude glycerol, an inevitable byproduct during biodiesel production, is emerging as a potential feedstock for fermentation, due to its availability and a reasonable price. The anaerobic digestion of glycerol derived from biodiesel manufacturing, in which COD was found to be 1010 g/kg, was studied in batch laboratory-scale reactors. Dissimilation of glycerol by Escherichia coli is strictly linked to their capacity to synthesize the highly reduced product 1,3-propanediol (1,3-PDO). The present study is focus on the development of adaptive mutant strains of Escherichia coli EC-MW (ATCC 11105), through microwave irradiation, at frequency 2.45 GHz and irradiation time 5 min pulse irradiation. The mutants were used for high bioethanol production from glycerol feedstock. Consequently, glycerol oxidative pathway (bioethanol) enhanced upon the parallel reduction in the 1,3-propanediol (1,3-PDO) pathway. The modified E. coli strains were able to increase bioethanol production upon fermentation reaching the level 280 g/L. |
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Article |
author |
Nomanbhay, S.M. Hussain, R. |
spellingShingle |
Nomanbhay, S.M. Hussain, R. Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
author_facet |
Nomanbhay, S.M. Hussain, R. |
author_sort |
Nomanbhay, S.M. |
title |
Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
title_short |
Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
title_full |
Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
title_fullStr |
Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
title_full_unstemmed |
Efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant Escherichia coli |
title_sort |
efficient production of bioethanol from waste glycerol using microwave irradiation induced mutant escherichia coli |
publishDate |
2017 |
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1644493824457703424 |
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13.211869 |