Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties
Cymodocea serrulata mediated titanium dioxide nanoparticles (TiO2 NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO2 NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO2 NPs has unifo...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
Academic Press
2025
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.uniten.dspace-36716 |
|---|---|
| record_format |
dspace |
| spelling |
my.uniten.dspace-367162025-03-03T15:44:08Z Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties Narayanan M. Srinivasan S. Gnanasekaran C. Ramachandran G. Chelliah C.K. Rajivgandhi G. Maruthupandy M. Quero F. Li W.-J. Hayder G. Khaled J.M. Arunachalam A. Manoharan N. 58700065200 58916230900 57225915806 57204557803 57225915270 57201259911 54391597500 36859665800 57219824895 56239664100 55683393900 58643178100 25221617900 Anti-Bacterial Agents Antioxidants Biofilms Metal Nanoparticles Methicillin-Resistant Staphylococcus aureus Nanoparticles Plant Extracts Titanium alkaloid flavonoid phosphotungstic acid phytochemical plant extract saponin tannin terpenoid titanium dioxide nanoparticle antiinfective agent antioxidant metal nanoparticle nanoparticle plant extract titanium titanium dioxide antibacterial activity antibiofilm activity antioxidant activity Article bacterial growth biofilm confocal laser scanning microscopy controlled study crystal structure DPPH radical scavenging assay energy dispersive X ray spectroscopy Fourier transform infrared spectroscopy Klebsiella pneumoniae methicillin resistant Staphylococcus aureus minimum inhibitory concentration nonhuman phytochemistry reliability scanning electron microscopy seagrass Staphylococcus aureus toxicity transmission electron microscopy Vibrio cholerae chemistry methicillin resistant Staphylococcus aureus Cymodocea serrulata mediated titanium dioxide nanoparticles (TiO2 NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO2 NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO2 NPs has uniform structure and spherical in shape with their size ranged from 58 to 117 nm. Antibacterial activity of TiO2 NPs against methicillin-resistant Staphylococcus aureus (MRSA) and Vibrio cholerae (V. cholerae), provided the zone of inhibition of 33.9 � 1.7 and 36.3 � 1.9 mm, respectively at 100 ?g/mL concentration. MIC of TiO2 NPs against MRSA and V. cholerae showed 84% and 87% inhibition at 180 ?g/mL and 160 ?g/mL respectively. Subsequently, the sub-MIC of V. cholerae demonstrated minimal or no impact on bacterial growth at concentration of 42.5 ?g/mL concentration. In addition, TiO2 NPs exhibited their ability to inhibit the biofilm forming V. cholerae which caused distinct morphological and intercellular damages analysed using CLSM and TEM. The antioxidant properties of TiO2 NPs were demonstrated through TAA and DPPH assays and exposed its scavenging activity with IC50 value of 36.42 and 68.85 ?g/mL which denotes its valuable antioxidant properties with potential health benefits. Importantly, the brine shrimp based lethality experiment yielded a low cytotoxic effect with 13% mortality at 100 ?g/mL. In conclusion, the multifaceted attributes of C. serrulata mediated TiO2 NPs encompassed the antibacterial, antioxidant and anti-biofilm inhibition effects with low cytotoxicity in nature were highlighted in this study and proved the bioderived TiO2 NPs could be used as a promising agent for biomedical applications. ? 2024 Elsevier Ltd Final 2025-03-03T07:44:08Z 2025-03-03T07:44:08Z 2024 Article 10.1016/j.micpath.2024.106595 2-s2.0-85186521777 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186521777&doi=10.1016%2fj.micpath.2024.106595&partnerID=40&md5=67db0b42785874a33fe0befdd6299671 https://irepository.uniten.edu.my/handle/123456789/36716 189 106595 Academic Press Scopus |
| institution |
Universiti Tenaga Nasional |
| building |
UNITEN Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Tenaga Nasional |
| content_source |
UNITEN Institutional Repository |
| url_provider |
http://dspace.uniten.edu.my/ |
| topic |
Anti-Bacterial Agents Antioxidants Biofilms Metal Nanoparticles Methicillin-Resistant Staphylococcus aureus Nanoparticles Plant Extracts Titanium alkaloid flavonoid phosphotungstic acid phytochemical plant extract saponin tannin terpenoid titanium dioxide nanoparticle antiinfective agent antioxidant metal nanoparticle nanoparticle plant extract titanium titanium dioxide antibacterial activity antibiofilm activity antioxidant activity Article bacterial growth biofilm confocal laser scanning microscopy controlled study crystal structure DPPH radical scavenging assay energy dispersive X ray spectroscopy Fourier transform infrared spectroscopy Klebsiella pneumoniae methicillin resistant Staphylococcus aureus minimum inhibitory concentration nonhuman phytochemistry reliability scanning electron microscopy seagrass Staphylococcus aureus toxicity transmission electron microscopy Vibrio cholerae chemistry methicillin resistant Staphylococcus aureus |
| spellingShingle |
Anti-Bacterial Agents Antioxidants Biofilms Metal Nanoparticles Methicillin-Resistant Staphylococcus aureus Nanoparticles Plant Extracts Titanium alkaloid flavonoid phosphotungstic acid phytochemical plant extract saponin tannin terpenoid titanium dioxide nanoparticle antiinfective agent antioxidant metal nanoparticle nanoparticle plant extract titanium titanium dioxide antibacterial activity antibiofilm activity antioxidant activity Article bacterial growth biofilm confocal laser scanning microscopy controlled study crystal structure DPPH radical scavenging assay energy dispersive X ray spectroscopy Fourier transform infrared spectroscopy Klebsiella pneumoniae methicillin resistant Staphylococcus aureus minimum inhibitory concentration nonhuman phytochemistry reliability scanning electron microscopy seagrass Staphylococcus aureus toxicity transmission electron microscopy Vibrio cholerae chemistry methicillin resistant Staphylococcus aureus Narayanan M. Srinivasan S. Gnanasekaran C. Ramachandran G. Chelliah C.K. Rajivgandhi G. Maruthupandy M. Quero F. Li W.-J. Hayder G. Khaled J.M. Arunachalam A. Manoharan N. Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| description |
Cymodocea serrulata mediated titanium dioxide nanoparticles (TiO2 NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO2 NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO2 NPs has uniform structure and spherical in shape with their size ranged from 58 to 117 nm. Antibacterial activity of TiO2 NPs against methicillin-resistant Staphylococcus aureus (MRSA) and Vibrio cholerae (V. cholerae), provided the zone of inhibition of 33.9 � 1.7 and 36.3 � 1.9 mm, respectively at 100 ?g/mL concentration. MIC of TiO2 NPs against MRSA and V. cholerae showed 84% and 87% inhibition at 180 ?g/mL and 160 ?g/mL respectively. Subsequently, the sub-MIC of V. cholerae demonstrated minimal or no impact on bacterial growth at concentration of 42.5 ?g/mL concentration. In addition, TiO2 NPs exhibited their ability to inhibit the biofilm forming V. cholerae which caused distinct morphological and intercellular damages analysed using CLSM and TEM. The antioxidant properties of TiO2 NPs were demonstrated through TAA and DPPH assays and exposed its scavenging activity with IC50 value of 36.42 and 68.85 ?g/mL which denotes its valuable antioxidant properties with potential health benefits. Importantly, the brine shrimp based lethality experiment yielded a low cytotoxic effect with 13% mortality at 100 ?g/mL. In conclusion, the multifaceted attributes of C. serrulata mediated TiO2 NPs encompassed the antibacterial, antioxidant and anti-biofilm inhibition effects with low cytotoxicity in nature were highlighted in this study and proved the bioderived TiO2 NPs could be used as a promising agent for biomedical applications. ? 2024 Elsevier Ltd |
| author2 |
58700065200 |
| author_facet |
58700065200 Narayanan M. Srinivasan S. Gnanasekaran C. Ramachandran G. Chelliah C.K. Rajivgandhi G. Maruthupandy M. Quero F. Li W.-J. Hayder G. Khaled J.M. Arunachalam A. Manoharan N. |
| format |
Article |
| author |
Narayanan M. Srinivasan S. Gnanasekaran C. Ramachandran G. Chelliah C.K. Rajivgandhi G. Maruthupandy M. Quero F. Li W.-J. Hayder G. Khaled J.M. Arunachalam A. Manoharan N. |
| author_sort |
Narayanan M. |
| title |
Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| title_short |
Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| title_full |
Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| title_fullStr |
Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| title_full_unstemmed |
Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| title_sort |
synthesis and characterization of marine seagrass (cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties |
| publisher |
Academic Press |
| publishDate |
2025 |
| _version_ |
1825816146348081152 |
| score |
13.244413 |