3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications
In this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspens...
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my.uniten.dspace-366012025-03-03T15:43:20Z 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications Ananth K.P. Jayram N.D. Muthusamy K. 55770359500 55965910000 57561339000 bioceramics calcium phosphate copolymer hydroxyapatite molecular scaffold surfactant Article biocompatibility biodegradation bone regeneration bone tissue cell adhesion cell differentiation cell proliferation chemical reaction kinetics controlled study human human cell implantation in vitro study load bearing MG-63 cell line micro-computed tomography nuclear magnetic resonance imaging osteoblast pH measurement pharmaceutics polymerization porosity refraction index scanning electron microscopy shear rate stereolithography surface property three dimensional printing tissue engineering transmission electron microscopy viscosity X ray diffraction In this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspension used in SLA exhibits significantly higher viscosity and is not environmentally friendly. Therefore, adequate preparation of a suspension with low viscosity and high solid loading is essential. In this paper, we optimized the effects of surfactant doses and solid loading on the BCp slurry, and initially examined the process parameters of photocuring, debinding, and sintering. The utilization of 9 wt % Disperbyk (BYK) with a 40 vol % loading of BCp bioceramics exhibited a reasonably low viscosity of 8.9 mPa�s at a shear level of 46.5 s?1. Functional and structural analyses confirmed that BCp was retained after photocuring and subsequent treatment, which were incorporated into the BYK dispersion. The 3D printed objects with different sintered temperatures, specifically at 1100 �C, 1200 �C, and 1300 �C, were further optimized. Additionally, the surface roughness, porosity, and mechanical properties of BCp green parts were systematically investigated. Most importantly, in vitro analysis of cell attachment, differentiation, and red alizarin analysis could support the application of bone regeneration. ? 2024 The Author(s) Final 2025-03-03T07:43:20Z 2025-03-03T07:43:20Z 2024 Article 10.1016/j.stlm.2024.100148 2-s2.0-85184601312 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184601312&doi=10.1016%2fj.stlm.2024.100148&partnerID=40&md5=11e856ab68774b7721416a2aaf81b957 https://irepository.uniten.edu.my/handle/123456789/36601 14 100148 All Open Access; Gold Open Access Elsevier Inc. Scopus |
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bioceramics calcium phosphate copolymer hydroxyapatite molecular scaffold surfactant Article biocompatibility biodegradation bone regeneration bone tissue cell adhesion cell differentiation cell proliferation chemical reaction kinetics controlled study human human cell implantation in vitro study load bearing MG-63 cell line micro-computed tomography nuclear magnetic resonance imaging osteoblast pH measurement pharmaceutics polymerization porosity refraction index scanning electron microscopy shear rate stereolithography surface property three dimensional printing tissue engineering transmission electron microscopy viscosity X ray diffraction |
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bioceramics calcium phosphate copolymer hydroxyapatite molecular scaffold surfactant Article biocompatibility biodegradation bone regeneration bone tissue cell adhesion cell differentiation cell proliferation chemical reaction kinetics controlled study human human cell implantation in vitro study load bearing MG-63 cell line micro-computed tomography nuclear magnetic resonance imaging osteoblast pH measurement pharmaceutics polymerization porosity refraction index scanning electron microscopy shear rate stereolithography surface property three dimensional printing tissue engineering transmission electron microscopy viscosity X ray diffraction Ananth K.P. Jayram N.D. Muthusamy K. 3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
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In this work, we developed and analyzed a biphasic calcium phosphate (BCP) bioceramic for bone regeneration using stereolithography (SLA). The SLA method is a promising additive manufacturing (AM) technique capable of creating BCp parts with high accuracy and efficiency. However, the ceramic suspension used in SLA exhibits significantly higher viscosity and is not environmentally friendly. Therefore, adequate preparation of a suspension with low viscosity and high solid loading is essential. In this paper, we optimized the effects of surfactant doses and solid loading on the BCp slurry, and initially examined the process parameters of photocuring, debinding, and sintering. The utilization of 9 wt % Disperbyk (BYK) with a 40 vol % loading of BCp bioceramics exhibited a reasonably low viscosity of 8.9 mPa�s at a shear level of 46.5 s?1. Functional and structural analyses confirmed that BCp was retained after photocuring and subsequent treatment, which were incorporated into the BYK dispersion. The 3D printed objects with different sintered temperatures, specifically at 1100 �C, 1200 �C, and 1300 �C, were further optimized. Additionally, the surface roughness, porosity, and mechanical properties of BCp green parts were systematically investigated. Most importantly, in vitro analysis of cell attachment, differentiation, and red alizarin analysis could support the application of bone regeneration. ? 2024 The Author(s) |
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55770359500 |
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55770359500 Ananth K.P. Jayram N.D. Muthusamy K. |
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Article |
| author |
Ananth K.P. Jayram N.D. Muthusamy K. |
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Ananth K.P. |
| title |
3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_short |
3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_full |
3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_fullStr |
3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_full_unstemmed |
3D-printed Biphasic Calcium Phosphate Scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| title_sort |
3d-printed biphasic calcium phosphate scaffold to augment cytocompatibility evaluation for load-bearing implant applications |
| publisher |
Elsevier Inc. |
| publishDate |
2025 |
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1825816067390308352 |
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13.244413 |