Staff View: Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water

Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water

The increased use of metallic nanoparticles has led to concern for environmental contamination and disruption in water quality. Therefore, effective screening of metallic nanoparticles is important for detecting metallic nanoparticles in aquatic environments. Biosensors offer several advantages, inc...

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Main Authors:	Thenarasu A., Chai M.K., Tan Y.H., Wong L.S., Rajamani R., Djearamane S.
Other Authors:	57884752400
Format:	Article
Published:	Technoscience Publications 2025
Subjects:	fluorescence genetic engineering microalga nanoparticle silver titanium water quality
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spelling	my.uniten.dspace-365582025-03-03T15:43:04Z Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water Thenarasu A. Chai M.K. Tan Y.H. Wong L.S. Rajamani R. Djearamane S. 57884752400 24172523100 57202000632 55819849800 57208159927 57202375412 fluorescence genetic engineering microalga nanoparticle silver titanium water quality The increased use of metallic nanoparticles has led to concern for environmental contamination and disruption in water quality. Therefore, effective screening of metallic nanoparticles is important for detecting metallic nanoparticles in aquatic environments. Biosensors offer several advantages, including high sensitivity to pollutants, short response time, energy efficiency, and low waste generation. In this study, a whole-cell biosensor was developed using microalga Chlorella vulgaris as a recognition element, and its fluorescence response was used as a measuring parameter for detecting the presence of titanium dioxide (TiO2) and silver (Ag) nanoparticles in water. The responses of C. vulgaris at the lag, exponential, and stationary phases to different concentrations of TiO2 and Ag nanoparticles were studied. The results showed that in TiO2 and Ag nanoparticles exposures, the highest fluorescence change (50-150%) was observed at the lag phase, whereas the lowest fluorescence change (40-75%) was observed at the stationary phase. A significant fluorescence change was observed in 15 min. The immobilized C. vulgaris under TiO2 and Ag nanoparticles exposures showed 30-180% higher fluorescence change than the negative control, indicating the potential of C. vulgaris as a biosensor for rapid detection of TiO2 and Ag nanoparticles in water. The mathematical modeling of the responses of C. vulgaris to TiO2 and Ag nanoparticles at 15 min of exposure with high R2 indicated that this biosensor is sensitive to the concentration tested (0.010-10.000 mg.L-1). Taken together, these results reveal that, for the first time, it is possible to detect TiO2 and Ag nanoparticles in water within a very short time using a microalgae-based biosensor. Moreover, no genetic engineering requirement makes this biosensor simple, economical, and free from the restriction on genetically modified microorganisms for environmental applications. ? 2024 Technoscience Publications. All rights reserved. Final 2025-03-03T07:43:04Z 2025-03-03T07:43:04Z 2024 Article 10.46488/NEPT.2024.v23i02.030 2-s2.0-85205026433 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85205026433&doi=10.46488%2fNEPT.2024.v23i02.030&partnerID=40&md5=634a6f81e27cd72c06955bc7eb96c9f3 https://irepository.uniten.edu.my/handle/123456789/36558 23 2 959 969 All Open Access; Gold Open Access Technoscience Publications Scopus
institution	Universiti Tenaga Nasional
building	UNITEN Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Tenaga Nasional
content_source	UNITEN Institutional Repository
url_provider	http://dspace.uniten.edu.my/
topic	fluorescence genetic engineering microalga nanoparticle silver titanium water quality
spellingShingle	fluorescence genetic engineering microalga nanoparticle silver titanium water quality Thenarasu A. Chai M.K. Tan Y.H. Wong L.S. Rajamani R. Djearamane S. Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
description	The increased use of metallic nanoparticles has led to concern for environmental contamination and disruption in water quality. Therefore, effective screening of metallic nanoparticles is important for detecting metallic nanoparticles in aquatic environments. Biosensors offer several advantages, including high sensitivity to pollutants, short response time, energy efficiency, and low waste generation. In this study, a whole-cell biosensor was developed using microalga Chlorella vulgaris as a recognition element, and its fluorescence response was used as a measuring parameter for detecting the presence of titanium dioxide (TiO2) and silver (Ag) nanoparticles in water. The responses of C. vulgaris at the lag, exponential, and stationary phases to different concentrations of TiO2 and Ag nanoparticles were studied. The results showed that in TiO2 and Ag nanoparticles exposures, the highest fluorescence change (50-150%) was observed at the lag phase, whereas the lowest fluorescence change (40-75%) was observed at the stationary phase. A significant fluorescence change was observed in 15 min. The immobilized C. vulgaris under TiO2 and Ag nanoparticles exposures showed 30-180% higher fluorescence change than the negative control, indicating the potential of C. vulgaris as a biosensor for rapid detection of TiO2 and Ag nanoparticles in water. The mathematical modeling of the responses of C. vulgaris to TiO2 and Ag nanoparticles at 15 min of exposure with high R2 indicated that this biosensor is sensitive to the concentration tested (0.010-10.000 mg.L-1). Taken together, these results reveal that, for the first time, it is possible to detect TiO2 and Ag nanoparticles in water within a very short time using a microalgae-based biosensor. Moreover, no genetic engineering requirement makes this biosensor simple, economical, and free from the restriction on genetically modified microorganisms for environmental applications. ? 2024 Technoscience Publications. All rights reserved.
author2	57884752400
author_facet	57884752400 Thenarasu A. Chai M.K. Tan Y.H. Wong L.S. Rajamani R. Djearamane S.
format	Article
author	Thenarasu A. Chai M.K. Tan Y.H. Wong L.S. Rajamani R. Djearamane S.
author_sort	Thenarasu A.
title	Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
title_short	Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
title_full	Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
title_fullStr	Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
title_full_unstemmed	Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water
title_sort	study of chlorella vulgaris from different growth phases as biosensor for detection of titanium and silver nanoparticles in water
publisher	Technoscience Publications
publishDate	2025
_version_	1825816187547680768
score	13.244109

Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water

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