Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology
In this study, PFC system is investigated to improve the concentration and yield of lysozyme. The research focused on an attempt to thoroughly construct an ice crystallizer with measurable and optimized design parameters for an efficient lysozyme protein concentration procedure because the productiv...
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my.uniten.dspace-362722025-03-03T15:41:46Z Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology Rashid T. Jusoh M. Zakaria Z.Y. Yahya N. Ansar S. Kiong T.S. Sher F. 56435809000 56065650700 57194233459 57203253973 16028093100 57216824752 57204792804 Cleaner production Crystallizers Sustainable development Thermal Engineering Thermal processing (foods) Design and optimization Energy Food production K-values Lysozyme proteins Multiple probe cryo-concentrator Nonthermal Protein concentrations Sustainable Sustainable food production and energy efficiency Biochemical engineering In this study, PFC system is investigated to improve the concentration and yield of lysozyme. The research focused on an attempt to thoroughly construct an ice crystallizer with measurable and optimized design parameters for an efficient lysozyme protein concentration procedure because the productivity of PFC is always an issue. A new Multiple Probe Cryo-Concentrator (MPCC) device was designed and successfully equipped with probes having a well-distributed cooled surface area for ice crystallization with proper internal cooling temperature control as well as a solution movement mechanism provided by a stirrer in the tank. The impact of different operating parameters is optimally investigated. Central Composite Design (CCD) is utilized to optimize PFC operating conditions and their response to partition constant (K-value) and solute yield. The results showed that a coolant temperature of -12 ?C, stirrer speed of 350 rpm, operation time of 40 min and initial concentration of 10 mg/mL gave the best K-value (0.132) and solute concentration yield (87.39 %). The design elements of the equipment are crucial in providing improved PFC performance. The study revealed that the PFC system designed and applied in this study can improve the lysozyme protein concentration as needed in the food and pharmaceutical industry. ? 2024 The Author(s) Final 2025-03-03T07:41:46Z 2025-03-03T07:41:46Z 2024 Article 10.1016/j.cep.2024.109975 2-s2.0-85203868384 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203868384&doi=10.1016%2fj.cep.2024.109975&partnerID=40&md5=b02b1f8e282cbcdfb38974e95c7aac5e https://irepository.uniten.edu.my/handle/123456789/36272 205 109975 All Open Access; Hybrid Gold Open Access Elsevier B.V. Scopus |
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Cleaner production Crystallizers Sustainable development Thermal Engineering Thermal processing (foods) Design and optimization Energy Food production K-values Lysozyme proteins Multiple probe cryo-concentrator Nonthermal Protein concentrations Sustainable Sustainable food production and energy efficiency Biochemical engineering |
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Cleaner production Crystallizers Sustainable development Thermal Engineering Thermal processing (foods) Design and optimization Energy Food production K-values Lysozyme proteins Multiple probe cryo-concentrator Nonthermal Protein concentrations Sustainable Sustainable food production and energy efficiency Biochemical engineering Rashid T. Jusoh M. Zakaria Z.Y. Yahya N. Ansar S. Kiong T.S. Sher F. Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
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In this study, PFC system is investigated to improve the concentration and yield of lysozyme. The research focused on an attempt to thoroughly construct an ice crystallizer with measurable and optimized design parameters for an efficient lysozyme protein concentration procedure because the productivity of PFC is always an issue. A new Multiple Probe Cryo-Concentrator (MPCC) device was designed and successfully equipped with probes having a well-distributed cooled surface area for ice crystallization with proper internal cooling temperature control as well as a solution movement mechanism provided by a stirrer in the tank. The impact of different operating parameters is optimally investigated. Central Composite Design (CCD) is utilized to optimize PFC operating conditions and their response to partition constant (K-value) and solute yield. The results showed that a coolant temperature of -12 ?C, stirrer speed of 350 rpm, operation time of 40 min and initial concentration of 10 mg/mL gave the best K-value (0.132) and solute concentration yield (87.39 %). The design elements of the equipment are crucial in providing improved PFC performance. The study revealed that the PFC system designed and applied in this study can improve the lysozyme protein concentration as needed in the food and pharmaceutical industry. ? 2024 The Author(s) |
| author2 |
56435809000 |
| author_facet |
56435809000 Rashid T. Jusoh M. Zakaria Z.Y. Yahya N. Ansar S. Kiong T.S. Sher F. |
| format |
Article |
| author |
Rashid T. Jusoh M. Zakaria Z.Y. Yahya N. Ansar S. Kiong T.S. Sher F. |
| author_sort |
Rashid T. |
| title |
Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| title_short |
Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| title_full |
Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| title_fullStr |
Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| title_full_unstemmed |
Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| title_sort |
design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology |
| publisher |
Elsevier B.V. |
| publishDate |
2025 |
| _version_ |
1825816017975115776 |
| score |
13.244413 |