The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production
Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective en...
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my.uniten.dspace-258692023-05-29T17:05:22Z The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production Hossain N. Hoong L.L. Barua P. Soudagar M.E.M. Mahlia T.M.I. 57193137546 57350790800 41860949000 57194384501 56997615100 Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches�cut into 2 cm2, blended with distilled water, and pretreated with diluted H2SO4�have been implemented, and pretreatment with diluted H2SO4 was the most effective. Hydrolysis with different concentrations�0.5 M, 1.0 M, 1.5 M, 2.0 M of H2SO4�was performed. The maximum glucose content was obtained at 2.0 M H2SO4 at 90 min reaction time, and glucose yield was 0.11 g glucose/g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5 mg, 10 mg, 15 mg, and 20 mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20 mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12 min. The distillation of bioethanol by rotary evaporator presented 0.79 ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%. � 2021, The Korean Institute of Chemical Engineers. Final 2023-05-29T09:05:21Z 2023-05-29T09:05:21Z 2021 Article 10.1007/s11814-021-0914-1 2-s2.0-85119691305 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119691305&doi=10.1007%2fs11814-021-0914-1&partnerID=40&md5=50ed9f730c3eb6716e339ceb97125058 https://irepository.uniten.edu.my/handle/123456789/25869 38 12 2493 2499 Springer Scopus |
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Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches�cut into 2 cm2, blended with distilled water, and pretreated with diluted H2SO4�have been implemented, and pretreatment with diluted H2SO4 was the most effective. Hydrolysis with different concentrations�0.5 M, 1.0 M, 1.5 M, 2.0 M of H2SO4�was performed. The maximum glucose content was obtained at 2.0 M H2SO4 at 90 min reaction time, and glucose yield was 0.11 g glucose/g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5 mg, 10 mg, 15 mg, and 20 mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20 mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12 min. The distillation of bioethanol by rotary evaporator presented 0.79 ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%. � 2021, The Korean Institute of Chemical Engineers. |
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57193137546 |
| author_facet |
57193137546 Hossain N. Hoong L.L. Barua P. Soudagar M.E.M. Mahlia T.M.I. |
| format |
Article |
| author |
Hossain N. Hoong L.L. Barua P. Soudagar M.E.M. Mahlia T.M.I. |
| spellingShingle |
Hossain N. Hoong L.L. Barua P. Soudagar M.E.M. Mahlia T.M.I. The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| author_sort |
Hossain N. |
| title |
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| title_short |
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| title_full |
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| title_fullStr |
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| title_full_unstemmed |
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| title_sort |
effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production |
| publisher |
Springer |
| publishDate |
2023 |
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1806424077004439552 |
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13.222552 |