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Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement

Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron ov...

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Main Authors: Lim W.F., Abdullah M., Ho K.L., Yap B.K., Lai M.I.
Other Authors: 50061393300
Format: Article
Published: Universiti Putra Malaysia Press 2023
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spelling my.uniten.dspace-251702023-05-29T16:07:06Z Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement Lim W.F. Abdullah M. Ho K.L. Yap B.K. Lai M.I. 50061393300 55666914500 18233493700 26649255900 13605357700 Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release. � 2020 UPM Press. All rights reserved. Final 2023-05-29T08:07:06Z 2023-05-29T08:07:06Z 2020 Article 2-s2.0-85099388878 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099388878&partnerID=40&md5=9aa54e9b0978a7ee3f13ff4f996c9c79 https://irepository.uniten.edu.my/handle/123456789/25170 16 52 57 Universiti Putra Malaysia Press Scopus
institution Universiti Tenaga Nasional
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country Malaysia
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description Introduction: Iron deficiency anaemia (IDA) is the most common cause of anaemia worldwide. Determination of body iron status is necessary to diagnose IDA. This can be measured using a biochemistry assessment of the serum/ plasma. Plasma/serum iron quantitation is also important in diagnosing iron overload disorders. However, iron studies are limited due to high cost and lack of access to biochemical analysers. Therefore, a cost- and technical-effective method is needed to measure human plasma iron concentration. Plasma iron is mainly transferrin-bound and an acidic plasmic condition is necessary to release the iron. This study investigated various candidate acid salts to achieve the acidic condition needed for plasma iron release. Method: Ten powdered or crystallised acid salts were studied for their water solubility as well as their pH reduction capability in revised simulated body fluid (r-SBF) and commercially available human plasma without any change in colour or form. Results: Six acid salts studied were discontinued from further investigation because they were insoluble in water. Another two candidates were unsuitable as they precipitated in r-SBF and human plasma. Maleic acid formed a jelly-like texture after a certain amount of time in human plasma. Only citric acid met all the criteria of a suitable acid salt to be investigated further as part of the reagent for a spontaneous plasma iron measurement. Conclusion: Citric acid, which is a colourless and odourless acid salt, was selected to lower the human plasma pH to an acidic condition for transferrin-bound iron release. � 2020 UPM Press. All rights reserved.
author2 50061393300
author_facet 50061393300
Lim W.F.
Abdullah M.
Ho K.L.
Yap B.K.
Lai M.I.
format Article
author Lim W.F.
Abdullah M.
Ho K.L.
Yap B.K.
Lai M.I.
spellingShingle Lim W.F.
Abdullah M.
Ho K.L.
Yap B.K.
Lai M.I.
Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
author_sort Lim W.F.
title Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
title_short Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
title_full Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
title_fullStr Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
title_full_unstemmed Selection of acid salts: A critical step in creating an acidic condition for plasma iron release and measurement
title_sort selection of acid salts: a critical step in creating an acidic condition for plasma iron release and measurement
publisher Universiti Putra Malaysia Press
publishDate 2023
_version_ 1806424472333320192
score 13.211869

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