Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology
Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was p...
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my.uniten.dspace-234842023-05-29T14:40:54Z Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology Sebayang A.H. Hassan M.H. Ong H.C. Dharma S. Silitonga A.S. Kusumo F. Mahlia T.M.I. Bahar A.H. 39262519300 9232771700 55310784800 57217370281 39262559400 56611974900 56997615100 56495544700 Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, ?-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g ?-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. Final 2023-05-29T06:40:54Z 2023-05-29T06:40:54Z 2017 Article 10.3390/en10010035 2-s2.0-85009275327 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009275327&doi=10.3390%2fen10010035&partnerID=40&md5=fdca88616dbac14864703544dd3a9c38 https://irepository.uniten.edu.my/handle/123456789/23484 10 1 35 All Open Access, Gold MDPI AG Scopus |
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Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, ?-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g ?-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. |
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39262519300 |
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39262519300 Sebayang A.H. Hassan M.H. Ong H.C. Dharma S. Silitonga A.S. Kusumo F. Mahlia T.M.I. Bahar A.H. |
| format |
Article |
| author |
Sebayang A.H. Hassan M.H. Ong H.C. Dharma S. Silitonga A.S. Kusumo F. Mahlia T.M.I. Bahar A.H. |
| spellingShingle |
Sebayang A.H. Hassan M.H. Ong H.C. Dharma S. Silitonga A.S. Kusumo F. Mahlia T.M.I. Bahar A.H. Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| author_sort |
Sebayang A.H. |
| title |
Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| title_short |
Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| title_full |
Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| title_fullStr |
Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| title_full_unstemmed |
Optimization of reducing sugar production from Manihot glaziovii starch using response surface methodology |
| title_sort |
optimization of reducing sugar production from manihot glaziovii starch using response surface methodology |
| publisher |
MDPI AG |
| publishDate |
2023 |
| _version_ |
1806426209309949952 |
| score |
13.211869 |