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Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)

Sago plant stores its starch in the trunk. It is considered mature and ready to be harvested when reaches 6- 8 years of growth. However, there are instances where sago palms in plantations remain stunted even after 10-14 years, thus devoid of starch in its trunk. This is known as non-trunking sago...

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Main Authors: Hasnain, Hussain, Maswida, binti Mustafa Kamal, Anastasia S., Edward Atit, Siti Izyan Liyana, Kamarol, Yan, Wei Jie, Zainab, Ngaini, Jameel, Al-Obaidi, Yusmin, Mohd-Yusuf
Format: Conference or Workshop Item
Language:English
Published: 2012
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/9983/1/PROTEOMICS%20OF%20TRUNKING%20AND%20NON-TRUNKING%20SAGO%20PALM%20%28METROXYLON%20SAGU%20ROTTB.%29%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/9983/
http://www.researchgate.net/publication/280810224_PROTEOMICS_OF_TRUNKING_AND_NON-TRUNKING_SAGO_PALM_(METROXYLON_SAGU_ROTTB.)
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spelling my.unimas.ir.99832015-12-08T06:50:19Z http://ir.unimas.my/id/eprint/9983/ Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.) Hasnain, Hussain Maswida, binti Mustafa Kamal Anastasia S., Edward Atit Siti Izyan Liyana, Kamarol Yan, Wei Jie Zainab, Ngaini Jameel, Al-Obaidi Yusmin, Mohd-Yusuf S Agriculture (General) Sago plant stores its starch in the trunk. It is considered mature and ready to be harvested when reaches 6- 8 years of growth. However, there are instances where sago palms in plantations remain stunted even after 10-14 years, thus devoid of starch in its trunk. This is known as non-trunking sago palm. The nontrunking phenotype of Metroxylon sagu eliminates the economical value of the plant and this is one of the major concerns of sago plantation till this day. Our research group has embarked on efforts to identify molecular factors that contribute to the differences between two groups of sago palm including their proteomics. Part of the work involves comparative proteomics analysis of trunking and non-trunking sago palm leaf. Prior to that, protein extraction and preparation, protein separation by one- or two-dimensional electrophoresis (1-DE or 2-DE, respectively), followed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were described. Using 2D gel electrophoresis, MALDI-TOF and RP-HPLC, we have identified several proteins that showed differences in level of protein expression between the two groups of sago palm. 2012 Conference or Workshop Item NonPeerReviewed text en http://ir.unimas.my/id/eprint/9983/1/PROTEOMICS%20OF%20TRUNKING%20AND%20NON-TRUNKING%20SAGO%20PALM%20%28METROXYLON%20SAGU%20ROTTB.%29%20%28abstract%29.pdf Hasnain, Hussain and Maswida, binti Mustafa Kamal and Anastasia S., Edward Atit and Siti Izyan Liyana, Kamarol and Yan, Wei Jie and Zainab, Ngaini and Jameel, Al-Obaidi and Yusmin, Mohd-Yusuf (2012) Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.). In: 2nd ASEAN Sago Symposium 2012, UNIMAS, Kota Samarahan Advances in Sago Research and Development, Samarahan, Kuching. http://www.researchgate.net/publication/280810224_PROTEOMICS_OF_TRUNKING_AND_NON-TRUNKING_SAGO_PALM_(METROXYLON_SAGU_ROTTB.)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Hasnain, Hussain
Maswida, binti Mustafa Kamal
Anastasia S., Edward Atit
Siti Izyan Liyana, Kamarol
Yan, Wei Jie
Zainab, Ngaini
Jameel, Al-Obaidi
Yusmin, Mohd-Yusuf
Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
description Sago plant stores its starch in the trunk. It is considered mature and ready to be harvested when reaches 6- 8 years of growth. However, there are instances where sago palms in plantations remain stunted even after 10-14 years, thus devoid of starch in its trunk. This is known as non-trunking sago palm. The nontrunking phenotype of Metroxylon sagu eliminates the economical value of the plant and this is one of the major concerns of sago plantation till this day. Our research group has embarked on efforts to identify molecular factors that contribute to the differences between two groups of sago palm including their proteomics. Part of the work involves comparative proteomics analysis of trunking and non-trunking sago palm leaf. Prior to that, protein extraction and preparation, protein separation by one- or two-dimensional electrophoresis (1-DE or 2-DE, respectively), followed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were described. Using 2D gel electrophoresis, MALDI-TOF and RP-HPLC, we have identified several proteins that showed differences in level of protein expression between the two groups of sago palm.
format Conference or Workshop Item
author Hasnain, Hussain
Maswida, binti Mustafa Kamal
Anastasia S., Edward Atit
Siti Izyan Liyana, Kamarol
Yan, Wei Jie
Zainab, Ngaini
Jameel, Al-Obaidi
Yusmin, Mohd-Yusuf
author_facet Hasnain, Hussain
Maswida, binti Mustafa Kamal
Anastasia S., Edward Atit
Siti Izyan Liyana, Kamarol
Yan, Wei Jie
Zainab, Ngaini
Jameel, Al-Obaidi
Yusmin, Mohd-Yusuf
author_sort Hasnain, Hussain
title Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
title_short Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
title_full Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
title_fullStr Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
title_full_unstemmed Proteomics Of Trunking And Nontrunking Sago Palm (Metroxylon Sagu Rottb.)
title_sort proteomics of trunking and nontrunking sago palm (metroxylon sagu rottb.)
publishDate 2012
url http://ir.unimas.my/id/eprint/9983/1/PROTEOMICS%20OF%20TRUNKING%20AND%20NON-TRUNKING%20SAGO%20PALM%20%28METROXYLON%20SAGU%20ROTTB.%29%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/9983/
http://www.researchgate.net/publication/280810224_PROTEOMICS_OF_TRUNKING_AND_NON-TRUNKING_SAGO_PALM_(METROXYLON_SAGU_ROTTB.)
_version_ 1644510861648199680
score 13.211869

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