Staff View: Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)

Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)

Objective: To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus. Methods: A series of culture methods were used to obtain fungal isolates from field-collected bed bugs. Characteristics of the isolates such as colony...

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Main Authors:	Abdul Hafiz, Ab Majid, Abd Hafis, Abd Rahim, Zulaikha, Zahran, Nor Azliza, Ismail, Wardah, Abdul Rahman, Kartiekasari Syahidda, Mohammad Zubairi, Hamady, Dieng, Tomomitsu, Satho
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	Q Science (General) SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/9710/1/Morphological.pdf http://ir.unimas.my/id/eprint/9710/ http://www.sciencedirect.com/science/article/pii/S2221169115001550
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spelling	my.unimas.ir.97102022-01-26T07:03:48Z http://ir.unimas.my/id/eprint/9710/ Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae) Abdul Hafiz, Ab Majid Abd Hafis, Abd Rahim Zulaikha, Zahran Nor Azliza, Ismail Wardah, Abdul Rahman Kartiekasari Syahidda, Mohammad Zubairi Hamady, Dieng Tomomitsu, Satho Q Science (General) SB Plant culture Objective: To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus. Methods: A series of culture methods were used to obtain fungal isolates from field-collected bed bugs. Characteristics of the isolates such as colony appearance, mycelial texture and pigmen-tation were studied to explore their morphology. Isolates were also subjected to a PCRbased genotyping test. Results: There were noticeable differences in morphological characteristics among the four isolates. Conidial areas of one isolate were dark green, whereas those of the remaining colonies were olive-green, black or dark brown. Conidia of the dark green isolate were globose, while those of olive-green, black and dark brown isolates were globose to subglobose, globose to spherical and globose to subglobose/finely roughened, respectively. These morphological specificities and the molecular analyses showed that the fungal internal transcribed spacer ribosomal region and β-tubulin gene sequences of the isolates shared clade with Trichoderma and Aspergillus sequences. Conclusions: Overall, the new discovery of common pathogens in agricultural field developed in live bed bugs storage tank may initiate the use of biological agents in later years. Elsevier 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9710/1/Morphological.pdf Abdul Hafiz, Ab Majid and Abd Hafis, Abd Rahim and Zulaikha, Zahran and Nor Azliza, Ismail and Wardah, Abdul Rahman and Kartiekasari Syahidda, Mohammad Zubairi and Hamady, Dieng and Tomomitsu, Satho (2015) Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae). Asian Pacific Journal of Tropical Biomedicine, 5 (9). pp. 930-936. ISSN 22211691 http://www.sciencedirect.com/science/article/pii/S2221169115001550 doi:10.1016/j.apjtb.2015.04.012
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) SB Plant culture
spellingShingle	Q Science (General) SB Plant culture Abdul Hafiz, Ab Majid Abd Hafis, Abd Rahim Zulaikha, Zahran Nor Azliza, Ismail Wardah, Abdul Rahman Kartiekasari Syahidda, Mohammad Zubairi Hamady, Dieng Tomomitsu, Satho Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
description	Objective: To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus. Methods: A series of culture methods were used to obtain fungal isolates from field-collected bed bugs. Characteristics of the isolates such as colony appearance, mycelial texture and pigmen-tation were studied to explore their morphology. Isolates were also subjected to a PCRbased genotyping test. Results: There were noticeable differences in morphological characteristics among the four isolates. Conidial areas of one isolate were dark green, whereas those of the remaining colonies were olive-green, black or dark brown. Conidia of the dark green isolate were globose, while those of olive-green, black and dark brown isolates were globose to subglobose, globose to spherical and globose to subglobose/finely roughened, respectively. These morphological specificities and the molecular analyses showed that the fungal internal transcribed spacer ribosomal region and β-tubulin gene sequences of the isolates shared clade with Trichoderma and Aspergillus sequences. Conclusions: Overall, the new discovery of common pathogens in agricultural field developed in live bed bugs storage tank may initiate the use of biological agents in later years.
format	Article
author	Abdul Hafiz, Ab Majid Abd Hafis, Abd Rahim Zulaikha, Zahran Nor Azliza, Ismail Wardah, Abdul Rahman Kartiekasari Syahidda, Mohammad Zubairi Hamady, Dieng Tomomitsu, Satho
author_facet	Abdul Hafiz, Ab Majid Abd Hafis, Abd Rahim Zulaikha, Zahran Nor Azliza, Ismail Wardah, Abdul Rahman Kartiekasari Syahidda, Mohammad Zubairi Hamady, Dieng Tomomitsu, Satho
author_sort	Abdul Hafiz, Ab Majid
title	Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
title_short	Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
title_full	Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
title_fullStr	Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
title_full_unstemmed	Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
title_sort	morphological and molecular characterization of fungus isolated from tropical bed bugs in northern peninsular malaysia, cimex hemipterus (hemiptera: cimicidae)
publisher	Elsevier
publishDate	2015
url	http://ir.unimas.my/id/eprint/9710/1/Morphological.pdf http://ir.unimas.my/id/eprint/9710/ http://www.sciencedirect.com/science/article/pii/S2221169115001550
_version_	1724078468902158336
score	13.211869

Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)

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