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Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)

A rapid, specific and sensitive multi-residues method has been developed for the analysis of 6 pesticides in black pepper based on the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) solid-phase extraction (SPE) sample preparation method. In this work, QuEChERS method, which was origina...

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Main Author: Ching, Lee Fung
Format: Thesis
Language:English
Published: University Malaysia Sarawak, UNIMAS 2015
Subjects:
QD Chemistry
Online Access:http://ir.unimas.my/id/eprint/9410/1/Ching%20Lee%20Fung%20ft.pdf
http://ir.unimas.my/id/eprint/9410/
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spelling my.unimas.ir.94102023-04-28T08:35:08Z http://ir.unimas.my/id/eprint/9410/ Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum) Ching, Lee Fung QD Chemistry A rapid, specific and sensitive multi-residues method has been developed for the analysis of 6 pesticides in black pepper based on the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) solid-phase extraction (SPE) sample preparation method. In this work, QuEChERS method, which was originally developed for vegetable samples with high amount of water, was modified to improve the extraction of a wide range of pesticides in dried product such as black pepper. The residues are extracted from the sample with acetonitrile. The extracted organic phase containing the pesticides was further clean-up by eluting with miniaturized carbon activated column (for organophosphorous pesticides) or silica column (for organochlorine pesticides). The SPE method was chosen because less solvent is used, time for clean up can be significantly reduced and cross contamination can be minimized. The final extracts were analyzed by gas chromatography (GC) equipped with flame photometric detector (FPD for organophosphorous pesticides) or electron capture detector (ECD for organochlorine pesticides) or high performance liquid chromatography (HPLC) equipped with ultra-violet detector (for benomyl). The recoveries of pepper samples spiked at 0.1, 0.5 and 1.0 mg kg-1 were between 60% to 75% with relative standard deviation (RSD) of less than 8%. This extraction method provided a well-defined phase separation without dilution and achieved acceptable recoveries. This method was applied for screening of pesticide residues in peppers from Balai Ringin, Semongok and Malaysia Pepper Board. University Malaysia Sarawak, UNIMAS 2015 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/9410/1/Ching%20Lee%20Fung%20ft.pdf Ching, Lee Fung (2015) Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum). Masters thesis, University Malaysia Sarawak, UNIMAS.
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ching, Lee Fung
Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
description A rapid, specific and sensitive multi-residues method has been developed for the analysis of 6 pesticides in black pepper based on the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) solid-phase extraction (SPE) sample preparation method. In this work, QuEChERS method, which was originally developed for vegetable samples with high amount of water, was modified to improve the extraction of a wide range of pesticides in dried product such as black pepper. The residues are extracted from the sample with acetonitrile. The extracted organic phase containing the pesticides was further clean-up by eluting with miniaturized carbon activated column (for organophosphorous pesticides) or silica column (for organochlorine pesticides). The SPE method was chosen because less solvent is used, time for clean up can be significantly reduced and cross contamination can be minimized. The final extracts were analyzed by gas chromatography (GC) equipped with flame photometric detector (FPD for organophosphorous pesticides) or electron capture detector (ECD for organochlorine pesticides) or high performance liquid chromatography (HPLC) equipped with ultra-violet detector (for benomyl). The recoveries of pepper samples spiked at 0.1, 0.5 and 1.0 mg kg-1 were between 60% to 75% with relative standard deviation (RSD) of less than 8%. This extraction method provided a well-defined phase separation without dilution and achieved acceptable recoveries. This method was applied for screening of pesticide residues in peppers from Balai Ringin, Semongok and Malaysia Pepper Board.
format Thesis
author Ching, Lee Fung
author_facet Ching, Lee Fung
author_sort Ching, Lee Fung
title Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
title_short Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
title_full Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
title_fullStr Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
title_full_unstemmed Development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
title_sort development of a multi-residues method for the analysis of pesticides in peper (piper nigrum)
publisher University Malaysia Sarawak, UNIMAS
publishDate 2015
url http://ir.unimas.my/id/eprint/9410/1/Ching%20Lee%20Fung%20ft.pdf
http://ir.unimas.my/id/eprint/9410/
_version_ 1765301162640146432
score 13.211869

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