Representational difference analysis (RDA) for identification of molecular factors contributing to trunking and non trunking sago palm (metroxylon sagu)
Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in Sarawak. The potential use as starch resource for the food industry required an increase its production. Under certain conditions, some sago palms do not develop well and become stunted. This is considered as...
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| Format: | Thesis |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/9389/1/Siti%20Izyan.pdf http://ir.unimas.my/id/eprint/9389/ |
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| Summary: | Sago palm (Metroxylon sagu Rottb.) is an economic valuable crop and cultivated widely in
Sarawak. The potential use as starch resource for the food industry required an increase its
production. Under certain conditions, some sago palms do not develop well and become
stunted. This is considered as non-trunking sago palm. Morphologically, this type of sago
palm is not normal and this led to wastage of various resources and affects the starch
production. The research objective was to understand the molecular process that affect
non-trunking and starch synthesis in sago palm. Representational Difference Analysis was
performed to identify the differences between trunking and non-trunking sago palm
transcriptome. This subtractive hybridization technique detects the differences in gene
expression. After three rounds of hybridization, the sequences obtained were compared
with the sequences in NCBI database. Results showed the activated genes in response to
the non trunking sago palm have similarity to genes that are implicated in plant growth,
cell metabolism and salt or osmotic stress. The differential expressed genes in trunking
sago palm code for genes related to growth development and signalling in neutralizing the
imbalance in plant stress. The information obtained through this work can be used to
develop database related to trunking and non-trunking sago palm. |
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