Biodegradation of heterocyclic hydrocarbon using biological activated carbon
The disturbance on ecological system caused by environmental pollution has raised public concerns. Recently, heterocyclic hydrocarbon has been claimed as one of the major pollutants. Therefore, it is essential to develop an efficient system that is capable of degrading heterocyclic hydrocarbon. I...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8729/8/Biodegradation%20of%20Heterocyclic%20Hydrocarbon%20Using%20Biological%20Activated%20Carbon%20%28full%29.pdf http://ir.unimas.my/id/eprint/8729/ |
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| Summary: | The disturbance on ecological system caused by environmental pollution has raised public
concerns. Recently, heterocyclic hydrocarbon has been claimed as one of the major pollutants.
Therefore, it is essential to develop an efficient system that is capable of degrading heterocyclic
hydrocarbon. In this study, the positive Thalassospira Sp. Strain M02 that is capable of degrading
specific substrate was selected and grew in ONR7a liquid medium supplemented with heterocyclic
bydrocarbon. Activated carbon was introduced into the ONR7a medium together with the growth
of bacteria to develop biological activated carbon. The detection of bacteria growth on activated
carbon was carried out by serial dilution on every three days from day 0 to day 15. In the mean
while, the degradation of carbazole was quantified by using Gas Chromatography-Mass
Spechtrometry (GC-MS) method on every two days from day 0 to day 14. The colony forming
units (CFU) in the log phase was higher and the carbazole was degraded to a lower concentration in
the biological activated carbon unit as compared to control unit. As for the conclusion, the
biodegradation of carbazole using biological activated carbon has shown to be more effective than
the control unit by a difference of 94% degradation efficiency on day 12. |
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