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A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak

RafJlesia tuan-mudae is an endemic species of Sarawak. However, there is a no complete description of R. luanmudae. Field work was done at Gunung Pueh, Gunung Gading and other localities such as Kg. Temurang, Kg. Begu and Lanjak Entimau to sample the blooming R. luan mudae. Detailed measurements and...

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Bibliographic Details
Main Author: Noor Izah, Binti Shoparwe
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2013
Subjects:
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/8725/8/Noor%20Izah.pdf
http://ir.unimas.my/id/eprint/8725/
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spelling my.unimas.ir.87252023-11-13T07:37:36Z http://ir.unimas.my/id/eprint/8725/ A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak Noor Izah, Binti Shoparwe SB Plant culture RafJlesia tuan-mudae is an endemic species of Sarawak. However, there is a no complete description of R. luanmudae. Field work was done at Gunung Pueh, Gunung Gading and other localities such as Kg. Temurang, Kg. Begu and Lanjak Entimau to sample the blooming R. luan mudae. Detailed measurements and description of R. luanmudae were made. Voucher specimens of R. Illan-mudae from Gunung Pueh was preserved as wet specimen in spirit and deposited at Universiti Malaysia Sarawak Herbarium. Plant DNA materials of unknown Rajjlesia spp. were taken from bracts orland perigone lobe and stored in silica gel. The DNA material was extracted, amplified and sequenced using nuclear ITS region. Maximum Parsimony (MP) and Maximum Likelihood (ML) analyses were carried out to estimate the phylogenetic tree to help in species identification. From the results obtained, it showed that the unknown RafJlesia spp. is closely related to R. luan-mudae based on the Maximum Parsimony and Maximum Likelihood analyses with moderate bootstrap support. From the alignment obtained, it clearly showed that the sequence of the unknown RafJlesia spp. is similar to the known R. Illan-mudae from the GenBank except one mutation at site 787 that differentiate R. luan-mudae with the unknown RafJlesia spp. Therefore, the unknown species could probably be R. luan-mudae. However, more data are needed to confirm this result. Universiti Malaysia Sarawak, (UNIMAS) 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/8725/8/Noor%20Izah.pdf Noor Izah, Binti Shoparwe (2013) A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
spellingShingle SB Plant culture
Noor Izah, Binti Shoparwe
A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
description RafJlesia tuan-mudae is an endemic species of Sarawak. However, there is a no complete description of R. luanmudae. Field work was done at Gunung Pueh, Gunung Gading and other localities such as Kg. Temurang, Kg. Begu and Lanjak Entimau to sample the blooming R. luan mudae. Detailed measurements and description of R. luanmudae were made. Voucher specimens of R. Illan-mudae from Gunung Pueh was preserved as wet specimen in spirit and deposited at Universiti Malaysia Sarawak Herbarium. Plant DNA materials of unknown Rajjlesia spp. were taken from bracts orland perigone lobe and stored in silica gel. The DNA material was extracted, amplified and sequenced using nuclear ITS region. Maximum Parsimony (MP) and Maximum Likelihood (ML) analyses were carried out to estimate the phylogenetic tree to help in species identification. From the results obtained, it showed that the unknown RafJlesia spp. is closely related to R. luan-mudae based on the Maximum Parsimony and Maximum Likelihood analyses with moderate bootstrap support. From the alignment obtained, it clearly showed that the sequence of the unknown RafJlesia spp. is similar to the known R. Illan-mudae from the GenBank except one mutation at site 787 that differentiate R. luan-mudae with the unknown RafJlesia spp. Therefore, the unknown species could probably be R. luan-mudae. However, more data are needed to confirm this result.
format Final Year Project Report
author Noor Izah, Binti Shoparwe
author_facet Noor Izah, Binti Shoparwe
author_sort Noor Izah, Binti Shoparwe
title A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
title_short A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
title_full A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
title_fullStr A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
title_full_unstemmed A taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in Sarawak
title_sort taxonomic study on rafflesia tuan-mudae (rafflesiaceae) in sarawak
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2013
url http://ir.unimas.my/id/eprint/8725/8/Noor%20Izah.pdf
http://ir.unimas.my/id/eprint/8725/
_version_ 1783883480459902976
score 13.211869

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