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Development of test method for anti-amoebic activity

A study was carried out to develop a preliminary method of antiamoebic screening test. Collection water samples were conducted in less polluted pond at Sarawak Golf Club UNIMAS to isolate nonpathogenic amoeba. Stepwise optimisation to initial amoeba isolation protocol resulted in a protocol which wa...

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Main Author: Nurulhazwani, Bt Mohd Rafiee
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/8132/3/Development%20of%20Test%20Method%20for%20Antiamoebic%20Activity%20%28full%29.pdf
http://ir.unimas.my/id/eprint/8132/
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spelling my.unimas.ir.81322023-11-22T06:49:58Z http://ir.unimas.my/id/eprint/8132/ Development of test method for anti-amoebic activity Nurulhazwani, Bt Mohd Rafiee Q Science (General) A study was carried out to develop a preliminary method of antiamoebic screening test. Collection water samples were conducted in less polluted pond at Sarawak Golf Club UNIMAS to isolate nonpathogenic amoeba. Stepwise optimisation to initial amoeba isolation protocol resulted in a protocol which was applied successful to isolate and culture amoeba. The antiamoebic activity was conducted by treating free living amoebas with 50 ~g/ml concentration of Amino acid-Thiourea derivative. The viability of cells was compared with the treatment Thiourea control and NaCl cytotoxicity control with the same concentration. The antiamoebic activity was based on comparison of percentage of viable cells from 50 first views of counted cells under 100x magnification inverted microscope. Data was analysed using one-way ANOV A and significant data was analysed with Fisher's LSD test. Results of treatment showed toxicity of Amino acid-thiourea derivative on free living amoeba compared to NaCI was successfully developed. The prototype of the model antiamoebic activity screening test was established preliminary method for further detail study. The study suggests that antiamoebic activity of this compound may further be explored in the future by using higher concentration ofcompound. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/8132/3/Development%20of%20Test%20Method%20for%20Antiamoebic%20Activity%20%28full%29.pdf Nurulhazwani, Bt Mohd Rafiee (2012) Development of test method for anti-amoebic activity. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Nurulhazwani, Bt Mohd Rafiee
Development of test method for anti-amoebic activity
description A study was carried out to develop a preliminary method of antiamoebic screening test. Collection water samples were conducted in less polluted pond at Sarawak Golf Club UNIMAS to isolate nonpathogenic amoeba. Stepwise optimisation to initial amoeba isolation protocol resulted in a protocol which was applied successful to isolate and culture amoeba. The antiamoebic activity was conducted by treating free living amoebas with 50 ~g/ml concentration of Amino acid-Thiourea derivative. The viability of cells was compared with the treatment Thiourea control and NaCl cytotoxicity control with the same concentration. The antiamoebic activity was based on comparison of percentage of viable cells from 50 first views of counted cells under 100x magnification inverted microscope. Data was analysed using one-way ANOV A and significant data was analysed with Fisher's LSD test. Results of treatment showed toxicity of Amino acid-thiourea derivative on free living amoeba compared to NaCI was successfully developed. The prototype of the model antiamoebic activity screening test was established preliminary method for further detail study. The study suggests that antiamoebic activity of this compound may further be explored in the future by using higher concentration ofcompound.
format Final Year Project Report
author Nurulhazwani, Bt Mohd Rafiee
author_facet Nurulhazwani, Bt Mohd Rafiee
author_sort Nurulhazwani, Bt Mohd Rafiee
title Development of test method for anti-amoebic activity
title_short Development of test method for anti-amoebic activity
title_full Development of test method for anti-amoebic activity
title_fullStr Development of test method for anti-amoebic activity
title_full_unstemmed Development of test method for anti-amoebic activity
title_sort development of test method for anti-amoebic activity
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2012
url http://ir.unimas.my/id/eprint/8132/3/Development%20of%20Test%20Method%20for%20Antiamoebic%20Activity%20%28full%29.pdf
http://ir.unimas.my/id/eprint/8132/
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score 13.211869

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