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Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.

A study was carried out to isolate antibiotic producing microorganisms from xanthid crabs which were collected from Satang Island, Division of Kuching, Sarawak. Isolation procedure involved swabbing method on NA and PDA medium. Nine bacteria and four fungi were successfully isolated from xanthid cra...

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Main Author: Nur 'Izzah Fateha, Md Zaher
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
QR Microbiology
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/7899/4/NUR%20%E2%80%98IZZAH%20FATEHA%20BT%20MD%20ZAHER%20ft.pdf
http://ir.unimas.my/id/eprint/7899/
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spelling my.unimas.ir.78992024-01-05T08:19:18Z http://ir.unimas.my/id/eprint/7899/ Screening For Antibiotics of Marine Microorganisms from Xanthid Crab. Nur 'Izzah Fateha, Md Zaher QR Microbiology SH Aquaculture. Fisheries. Angling A study was carried out to isolate antibiotic producing microorganisms from xanthid crabs which were collected from Satang Island, Division of Kuching, Sarawak. Isolation procedure involved swabbing method on NA and PDA medium. Nine bacteria and four fungi were successfully isolated from xanthid crab. Preliminary screening for antibacterial activity was conducted using spot inoculation method for bacteria and agar overlay technique for fungi against Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes and Escherichia coli. Of the nine bacterial isolates obtained, five isolates (B1.4, B2.1, B2.3, B2.4 and B2.5) were positive against S. aureus, and S. typhi. Besides, three fungal isolates (B1.1, B1.4 and B2.1) showed antibacterial activity against all test bacteria. The positive isolates were subjected to a second antibacterial test by agar well method for bacteria and agar overlay technique for fungi. In second antibacterial test, four bacterial isolates (B1.4, B2.3, B2.4 and B2.5) and three fungal isolates (B1.1, B1.4 and B2.1) showed positive antibacterial activity. For antifungal test, B1.1 showed zone of inhibition against Fusarium sp., B1.4 and B2.1. Another two fungal isolates (B1.4 and B2.1) were inhibited by Fusarium sp. Moreover, five methanol extracts of bacterial and fungal isolates were tested for antibiotics activity by Kirby-Bauer disc-diffusion method. Findings revealed that two bacterial extracts (B2.4 and B2.5) showed positive inhibition against all test bacteria meanwhile, two fungal extracts (B1.4 and B2.1) have activity against S.aureus and none inhibition showed by B1.1. The highest diameter zone of inhibition was recorded against S.aureus (9mm) for fungal extract (B1.4). The fungal isolates were identified as Penicillium sp. (B1.4 and B2.1 meanwhile, B2.4 and B2.5 bacterial isolates were identified as Gram negative. The results indicate that the bacteria and fungi isolated from xanthid crabs produce potential antibacterial, antifungal and broad spectrum of antibiotics compounds. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7899/4/NUR%20%E2%80%98IZZAH%20FATEHA%20BT%20MD%20ZAHER%20ft.pdf Nur 'Izzah Fateha, Md Zaher (2010) Screening For Antibiotics of Marine Microorganisms from Xanthid Crab. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
SH Aquaculture. Fisheries. Angling
spellingShingle QR Microbiology
SH Aquaculture. Fisheries. Angling
Nur 'Izzah Fateha, Md Zaher
Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
description A study was carried out to isolate antibiotic producing microorganisms from xanthid crabs which were collected from Satang Island, Division of Kuching, Sarawak. Isolation procedure involved swabbing method on NA and PDA medium. Nine bacteria and four fungi were successfully isolated from xanthid crab. Preliminary screening for antibacterial activity was conducted using spot inoculation method for bacteria and agar overlay technique for fungi against Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes and Escherichia coli. Of the nine bacterial isolates obtained, five isolates (B1.4, B2.1, B2.3, B2.4 and B2.5) were positive against S. aureus, and S. typhi. Besides, three fungal isolates (B1.1, B1.4 and B2.1) showed antibacterial activity against all test bacteria. The positive isolates were subjected to a second antibacterial test by agar well method for bacteria and agar overlay technique for fungi. In second antibacterial test, four bacterial isolates (B1.4, B2.3, B2.4 and B2.5) and three fungal isolates (B1.1, B1.4 and B2.1) showed positive antibacterial activity. For antifungal test, B1.1 showed zone of inhibition against Fusarium sp., B1.4 and B2.1. Another two fungal isolates (B1.4 and B2.1) were inhibited by Fusarium sp. Moreover, five methanol extracts of bacterial and fungal isolates were tested for antibiotics activity by Kirby-Bauer disc-diffusion method. Findings revealed that two bacterial extracts (B2.4 and B2.5) showed positive inhibition against all test bacteria meanwhile, two fungal extracts (B1.4 and B2.1) have activity against S.aureus and none inhibition showed by B1.1. The highest diameter zone of inhibition was recorded against S.aureus (9mm) for fungal extract (B1.4). The fungal isolates were identified as Penicillium sp. (B1.4 and B2.1 meanwhile, B2.4 and B2.5 bacterial isolates were identified as Gram negative. The results indicate that the bacteria and fungi isolated from xanthid crabs produce potential antibacterial, antifungal and broad spectrum of antibiotics compounds.
format Final Year Project Report
author Nur 'Izzah Fateha, Md Zaher
author_facet Nur 'Izzah Fateha, Md Zaher
author_sort Nur 'Izzah Fateha, Md Zaher
title Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
title_short Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
title_full Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
title_fullStr Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
title_full_unstemmed Screening For Antibiotics of Marine Microorganisms from Xanthid Crab.
title_sort screening for antibiotics of marine microorganisms from xanthid crab.
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2010
url http://ir.unimas.my/id/eprint/7899/4/NUR%20%E2%80%98IZZAH%20FATEHA%20BT%20MD%20ZAHER%20ft.pdf
http://ir.unimas.my/id/eprint/7899/
_version_ 1787519562554540032
score 13.211869

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