Cover Image

Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes

Oil palm empty fruit bunches (EFB) and pineapple wastes are the most abundant cellulosic wastes in our country. In this research, a total of 14 strains of bacteria that are able to utilize carboxymethyl cellulose have been successfully isolated from decomposed oil palm empty fruit bunches and pineap...

Full description

Saved in:
Bibliographic Details
Main Author: Mazidatul Ashiqeen Balqiah, Mohamad Lazim
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2010
Subjects:
GE Environmental Sciences
Online Access:http://ir.unimas.my/id/eprint/7871/3/MAZIDATUL%20full.pdf
http://ir.unimas.my/id/eprint/7871/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.7871
record_format eprints
spelling my.unimas.ir.78712023-08-29T08:46:17Z http://ir.unimas.my/id/eprint/7871/ Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes Mazidatul Ashiqeen Balqiah, Mohamad Lazim GE Environmental Sciences Oil palm empty fruit bunches (EFB) and pineapple wastes are the most abundant cellulosic wastes in our country. In this research, a total of 14 strains of bacteria that are able to utilize carboxymethyl cellulose have been successfully isolated from decomposed oil palm empty fruit bunches and pineapple waste. Some of the isolates were also found to be resistance to heavy metals and antibiotics. Morphological characterization using gram staining and some biochemical test were carried out to determine the genera of the strains. Results showed that the strains belong to genera Azotobacter, Acetobacter, Bacillus, Cellulomonas, Micrococcus and Staphylococcus. To indicate the cellulolytic activities, hydrolysis zone on 0.5% carboxymethylcellulose (CMC) agar was measured. DNS assay was performed for quantifying the reducing sugar produced and P6 1 strain found as the best cellulose degrading bacteria. Plasmid DNA isolation attempted on the best five strains to detect presence of plasmid that may carry cellulose degrading gene. However, none of strains showed the presence of plasmid DNA. Universiti Malaysia Sarawak (UNIMAS) 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7871/3/MAZIDATUL%20full.pdf Mazidatul Ashiqeen Balqiah, Mohamad Lazim (2010) Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic GE Environmental Sciences
spellingShingle GE Environmental Sciences
Mazidatul Ashiqeen Balqiah, Mohamad Lazim
Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
description Oil palm empty fruit bunches (EFB) and pineapple wastes are the most abundant cellulosic wastes in our country. In this research, a total of 14 strains of bacteria that are able to utilize carboxymethyl cellulose have been successfully isolated from decomposed oil palm empty fruit bunches and pineapple waste. Some of the isolates were also found to be resistance to heavy metals and antibiotics. Morphological characterization using gram staining and some biochemical test were carried out to determine the genera of the strains. Results showed that the strains belong to genera Azotobacter, Acetobacter, Bacillus, Cellulomonas, Micrococcus and Staphylococcus. To indicate the cellulolytic activities, hydrolysis zone on 0.5% carboxymethylcellulose (CMC) agar was measured. DNS assay was performed for quantifying the reducing sugar produced and P6 1 strain found as the best cellulose degrading bacteria. Plasmid DNA isolation attempted on the best five strains to detect presence of plasmid that may carry cellulose degrading gene. However, none of strains showed the presence of plasmid DNA.
format Final Year Project Report
author Mazidatul Ashiqeen Balqiah, Mohamad Lazim
author_facet Mazidatul Ashiqeen Balqiah, Mohamad Lazim
author_sort Mazidatul Ashiqeen Balqiah, Mohamad Lazim
title Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
title_short Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
title_full Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
title_fullStr Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
title_full_unstemmed Isolation and Characterization of Cellulose Degrading Bacteria From Decomposing Empty Fruit Bunches (EFB) and Pineapple Wastes
title_sort isolation and characterization of cellulose degrading bacteria from decomposing empty fruit bunches (efb) and pineapple wastes
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2010
url http://ir.unimas.my/id/eprint/7871/3/MAZIDATUL%20full.pdf
http://ir.unimas.my/id/eprint/7871/
_version_ 1775627173779996672
score 13.211869

Similar Items