Cover Image

Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system

Colorectal cancer progresses in a stepwise manner and is found to be linked with the accumulating mutations (Franks and Teich, 1993). The recent discovery found that the expression of human 20-kDa Myosin Light Chain (MLC-2) gene is downregulated in the tumour colon tissue compared to the normal colo...

Full description

Saved in:
Bibliographic Details
Main Author: Chia, Christine Pek Imm
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2007
Subjects:
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/7867/4/Christine%20Chia%20Pek%20Imm%20ft.pdf
http://ir.unimas.my/id/eprint/7867/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.7867
record_format eprints
spelling my.unimas.ir.78672023-02-08T08:49:58Z http://ir.unimas.my/id/eprint/7867/ Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system Chia, Christine Pek Imm R Medicine (General) Colorectal cancer progresses in a stepwise manner and is found to be linked with the accumulating mutations (Franks and Teich, 1993). The recent discovery found that the expression of human 20-kDa Myosin Light Chain (MLC-2) gene is downregulated in the tumour colon tissue compared to the normal colon tissue (Sim et al., 2006). The objective of this study is to screen for mutation in the MLC-2 gene of the normal and tumour samples by restriction endonucleases. Total RNA of both samples was used as the starting material and was transcribed into cDNA using M-MLV reverse transcriptase. The 251 bp MLC-2 gene was successfully amplified by polymerase chain reaction and was subsequently digested with 15 different restriction endonucleases. The digestion yielded no abnormal cleavage of the MLC-2 gene fragment between tumour and normal colon samples. Universiti Malaysia Sarawak, UNIMAS 2007 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7867/4/Christine%20Chia%20Pek%20Imm%20ft.pdf Chia, Christine Pek Imm (2007) Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Chia, Christine Pek Imm
Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
description Colorectal cancer progresses in a stepwise manner and is found to be linked with the accumulating mutations (Franks and Teich, 1993). The recent discovery found that the expression of human 20-kDa Myosin Light Chain (MLC-2) gene is downregulated in the tumour colon tissue compared to the normal colon tissue (Sim et al., 2006). The objective of this study is to screen for mutation in the MLC-2 gene of the normal and tumour samples by restriction endonucleases. Total RNA of both samples was used as the starting material and was transcribed into cDNA using M-MLV reverse transcriptase. The 251 bp MLC-2 gene was successfully amplified by polymerase chain reaction and was subsequently digested with 15 different restriction endonucleases. The digestion yielded no abnormal cleavage of the MLC-2 gene fragment between tumour and normal colon samples.
format Final Year Project Report
author Chia, Christine Pek Imm
author_facet Chia, Christine Pek Imm
author_sort Chia, Christine Pek Imm
title Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
title_short Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
title_full Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
title_fullStr Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
title_full_unstemmed Isolation and restriction endonuclease (RE) site characterization of human 20-KDA myosin light chain 2 (MLC-2) gene from human colorectal system
title_sort isolation and restriction endonuclease (re) site characterization of human 20-kda myosin light chain 2 (mlc-2) gene from human colorectal system
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2007
url http://ir.unimas.my/id/eprint/7867/4/Christine%20Chia%20Pek%20Imm%20ft.pdf
http://ir.unimas.my/id/eprint/7867/
_version_ 1758582481073733632
score 13.211869

Similar Items