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In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship

The lignin biosynthesis genes are highly conserved throughout vascular plants and thus it can provide useful information to better understand the evolution of land plants. Three Angiosperm trees namely Arabidopsis thaliana, Populus trichocarpa, Eucalyptus globulus, and a gymnosperm tree, Pinus taeda...

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Main Author: Wong, Lai Kuan
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
GE Environmental Sciences
Online Access:http://ir.unimas.my/id/eprint/7866/1/Lai%20Kuan%28%2024%20pgs%29.pdf
http://ir.unimas.my/id/eprint/7866/4/Lai%20Kuan%28ft%29.pdf
http://ir.unimas.my/id/eprint/7866/
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spelling my.unimas.ir.78662024-08-23T02:25:05Z http://ir.unimas.my/id/eprint/7866/ In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship Wong, Lai Kuan GE Environmental Sciences The lignin biosynthesis genes are highly conserved throughout vascular plants and thus it can provide useful information to better understand the evolution of land plants. Three Angiosperm trees namely Arabidopsis thaliana, Populus trichocarpa, Eucalyptus globulus, and a gymnosperm tree, Pinus taeda were selected in this study. Motif and domain for each gene were identified by scanning it with PROSITE and Pfam databases. In this study, only six of the genes: 4CL, CAD, PAL, C3H, C4H and F5H have motif identified and represented in regular expression. The motif in regular expression can be used to estimate the protein function. Domain analysis showed that two lignin proteins; COMT, CAD are multidomains proteins while C3H, C4H and F5H shared the same domain, p450 which involve in oxidative metabolism of natural compounds. The phylogenetic analyses were carried out using two different approaches implemented in MEGA software: distance based (NJ) and character based (MP). The phylogenetic analysis of lignin genes showed the divergence between angiosperm and gymnosperm species. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7866/1/Lai%20Kuan%28%2024%20pgs%29.pdf text en http://ir.unimas.my/id/eprint/7866/4/Lai%20Kuan%28ft%29.pdf Wong, Lai Kuan (2010) In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic GE Environmental Sciences
spellingShingle GE Environmental Sciences
Wong, Lai Kuan
In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
description The lignin biosynthesis genes are highly conserved throughout vascular plants and thus it can provide useful information to better understand the evolution of land plants. Three Angiosperm trees namely Arabidopsis thaliana, Populus trichocarpa, Eucalyptus globulus, and a gymnosperm tree, Pinus taeda were selected in this study. Motif and domain for each gene were identified by scanning it with PROSITE and Pfam databases. In this study, only six of the genes: 4CL, CAD, PAL, C3H, C4H and F5H have motif identified and represented in regular expression. The motif in regular expression can be used to estimate the protein function. Domain analysis showed that two lignin proteins; COMT, CAD are multidomains proteins while C3H, C4H and F5H shared the same domain, p450 which involve in oxidative metabolism of natural compounds. The phylogenetic analyses were carried out using two different approaches implemented in MEGA software: distance based (NJ) and character based (MP). The phylogenetic analysis of lignin genes showed the divergence between angiosperm and gymnosperm species.
format Final Year Project Report
author Wong, Lai Kuan
author_facet Wong, Lai Kuan
author_sort Wong, Lai Kuan
title In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
title_short In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
title_full In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
title_fullStr In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
title_full_unstemmed In Silico Analysis of Candidate Genes Involved in Lignin Biosynthesis from Woody Species and Phylogenetic Relationship
title_sort in silico analysis of candidate genes involved in lignin biosynthesis from woody species and phylogenetic relationship
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2010
url http://ir.unimas.my/id/eprint/7866/1/Lai%20Kuan%28%2024%20pgs%29.pdf
http://ir.unimas.my/id/eprint/7866/4/Lai%20Kuan%28ft%29.pdf
http://ir.unimas.my/id/eprint/7866/
_version_ 1808981455448047616
score 13.211869

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