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Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers

This study was done to examine the efficacy of microsatellite primers developed from two puffer fishes (Takifugu rubripes and Takifugu obscures) through cross-species amplification in Lagocephalus spadiceus. Samples from 2 different geographical populations (1=Kudat & 2=Tawau) in the coastal are...

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Main Author: Tang, Janet Hung Sing
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/7850/8/Genetic%20Characterization%20of%20Lagocephalus%20spadiceus%20Among%20Two%20Populations%20Using%20Micrpsatellite%20DNA%20Markers.pdf
http://ir.unimas.my/id/eprint/7850/
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spelling my.unimas.ir.78502024-08-26T08:08:29Z http://ir.unimas.my/id/eprint/7850/ Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers Tang, Janet Hung Sing SH Aquaculture. Fisheries. Angling This study was done to examine the efficacy of microsatellite primers developed from two puffer fishes (Takifugu rubripes and Takifugu obscures) through cross-species amplification in Lagocephalus spadiceus. Samples from 2 different geographical populations (1=Kudat & 2=Tawau) in the coastal area of Sabah were tested. PCR optimization was done to test a total of 20 primers from genus Takifugu and 6 primers were produced amplified products. They were subsequently used for screening in larger sample size. However, only one primer (P3) was successfully tested for polymorphism while the other five primers could not been tested due to shortage of chemicals for PCR. P3 primer produced low level of polymorphism when tested using 53 samples in both of the populations. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7850/8/Genetic%20Characterization%20of%20Lagocephalus%20spadiceus%20Among%20Two%20Populations%20Using%20Micrpsatellite%20DNA%20Markers.pdf Tang, Janet Hung Sing (2010) Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Tang, Janet Hung Sing
Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
description This study was done to examine the efficacy of microsatellite primers developed from two puffer fishes (Takifugu rubripes and Takifugu obscures) through cross-species amplification in Lagocephalus spadiceus. Samples from 2 different geographical populations (1=Kudat & 2=Tawau) in the coastal area of Sabah were tested. PCR optimization was done to test a total of 20 primers from genus Takifugu and 6 primers were produced amplified products. They were subsequently used for screening in larger sample size. However, only one primer (P3) was successfully tested for polymorphism while the other five primers could not been tested due to shortage of chemicals for PCR. P3 primer produced low level of polymorphism when tested using 53 samples in both of the populations.
format Final Year Project Report
author Tang, Janet Hung Sing
author_facet Tang, Janet Hung Sing
author_sort Tang, Janet Hung Sing
title Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
title_short Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
title_full Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
title_fullStr Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
title_full_unstemmed Genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
title_sort genetic characterization of lagocephalus spadiceus among two populations using microsatellite dna markers
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2010
url http://ir.unimas.my/id/eprint/7850/8/Genetic%20Characterization%20of%20Lagocephalus%20spadiceus%20Among%20Two%20Populations%20Using%20Micrpsatellite%20DNA%20Markers.pdf
http://ir.unimas.my/id/eprint/7850/
_version_ 1808981454992965632
score 13.211869

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