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Comparative analysis of chloroplast DNA of crytocoryne species

This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the...

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Main Author: Muhammad Mustaqim, Haji Shamsudin
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf
http://ir.unimas.my/id/eprint/7824/
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spelling my.unimas.ir.78242023-08-29T08:39:53Z http://ir.unimas.my/id/eprint/7824/ Comparative analysis of chloroplast DNA of crytocoryne species Muhammad Mustaqim, Haji Shamsudin Q Science (General) This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the hybrid through molecular work provides useful information for taxonomy study. One new hybrid of C. purpurea and three possible mother plants of the hybrid; C. cordata var. zonata, C. ferruginea and C. pallidinervia have been used. Polymorphism in chloroplast DNA in these Cryptocoryne species is determined through PCR amplification of rbcL gene sequence coded rubisco synthesis using primers rbcL-1 (5’-TGTCACCAAAAACAGAGACT-3’) and rbcL-2 (5’-TTCCATACTTCACAAGCAGC-3’), and restriction fragment digestion using several restriction endonucleases. Polymorphisms of certain restriction sites were obtained. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf Muhammad Mustaqim, Haji Shamsudin (2010) Comparative analysis of chloroplast DNA of crytocoryne species. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Muhammad Mustaqim, Haji Shamsudin
Comparative analysis of chloroplast DNA of crytocoryne species
description This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the hybrid through molecular work provides useful information for taxonomy study. One new hybrid of C. purpurea and three possible mother plants of the hybrid; C. cordata var. zonata, C. ferruginea and C. pallidinervia have been used. Polymorphism in chloroplast DNA in these Cryptocoryne species is determined through PCR amplification of rbcL gene sequence coded rubisco synthesis using primers rbcL-1 (5’-TGTCACCAAAAACAGAGACT-3’) and rbcL-2 (5’-TTCCATACTTCACAAGCAGC-3’), and restriction fragment digestion using several restriction endonucleases. Polymorphisms of certain restriction sites were obtained.
format Final Year Project Report
author Muhammad Mustaqim, Haji Shamsudin
author_facet Muhammad Mustaqim, Haji Shamsudin
author_sort Muhammad Mustaqim, Haji Shamsudin
title Comparative analysis of chloroplast DNA of crytocoryne species
title_short Comparative analysis of chloroplast DNA of crytocoryne species
title_full Comparative analysis of chloroplast DNA of crytocoryne species
title_fullStr Comparative analysis of chloroplast DNA of crytocoryne species
title_full_unstemmed Comparative analysis of chloroplast DNA of crytocoryne species
title_sort comparative analysis of chloroplast dna of crytocoryne species
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2010
url http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf
http://ir.unimas.my/id/eprint/7824/
_version_ 1775627173039702016
score 13.211869

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