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Transformation of isochorismate synthase in fungal system

Agrobacterium-mediated transformation is a powerful tool that allows the introduction of particular genetic changes in an organism. Transformation of fungi, Aspergillus niger is necessary to fulfill the increasing global industrial demand. Isochorismate synthase gene from Morinda citrifolia is im...

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Main Author: Lim, Pei Wen
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2013
Subjects:
Q Science (General)
QH301 Biology
QH426 Genetics
Online Access:http://ir.unimas.my/id/eprint/7625/1/Lim%20Pei%20Wen%20ft.pdf
http://ir.unimas.my/id/eprint/7625/
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spelling my.unimas.ir.76252024-03-05T08:45:12Z http://ir.unimas.my/id/eprint/7625/ Transformation of isochorismate synthase in fungal system Lim, Pei Wen Q Science (General) QH301 Biology QH426 Genetics Agrobacterium-mediated transformation is a powerful tool that allows the introduction of particular genetic changes in an organism. Transformation of fungi, Aspergillus niger is necessary to fulfill the increasing global industrial demand. Isochorismate synthase gene from Morinda citrifolia is important in the involvement of salicylic acid which plays a role in plant immunological system. In this study, the isolated plasmids pGSA1131/ICS were successfully transformed into Agrobacterium tumefaciens cells. A. tumefaciens carrying pGSA1131/ICS containing bacterial BAR-resistant gene have been used in A. niger transformation. Different concentration of growth media containing different concentration of selective agent, Basta, were used in the transformation to obtain a maximum yield of transformants. The growth of mycelia on top agar of PDA plates containing reduced concentration of Basta at 10 mg/L. The extracted fungal DNA was examined via PCR. A successful transformation of A. niger was achieved. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7625/1/Lim%20Pei%20Wen%20ft.pdf Lim, Pei Wen (2013) Transformation of isochorismate synthase in fungal system. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QH301 Biology
QH426 Genetics
spellingShingle Q Science (General)
QH301 Biology
QH426 Genetics
Lim, Pei Wen
Transformation of isochorismate synthase in fungal system
description Agrobacterium-mediated transformation is a powerful tool that allows the introduction of particular genetic changes in an organism. Transformation of fungi, Aspergillus niger is necessary to fulfill the increasing global industrial demand. Isochorismate synthase gene from Morinda citrifolia is important in the involvement of salicylic acid which plays a role in plant immunological system. In this study, the isolated plasmids pGSA1131/ICS were successfully transformed into Agrobacterium tumefaciens cells. A. tumefaciens carrying pGSA1131/ICS containing bacterial BAR-resistant gene have been used in A. niger transformation. Different concentration of growth media containing different concentration of selective agent, Basta, were used in the transformation to obtain a maximum yield of transformants. The growth of mycelia on top agar of PDA plates containing reduced concentration of Basta at 10 mg/L. The extracted fungal DNA was examined via PCR. A successful transformation of A. niger was achieved.
format Final Year Project Report
author Lim, Pei Wen
author_facet Lim, Pei Wen
author_sort Lim, Pei Wen
title Transformation of isochorismate synthase in fungal system
title_short Transformation of isochorismate synthase in fungal system
title_full Transformation of isochorismate synthase in fungal system
title_fullStr Transformation of isochorismate synthase in fungal system
title_full_unstemmed Transformation of isochorismate synthase in fungal system
title_sort transformation of isochorismate synthase in fungal system
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2013
url http://ir.unimas.my/id/eprint/7625/1/Lim%20Pei%20Wen%20ft.pdf
http://ir.unimas.my/id/eprint/7625/
_version_ 1793163046832570368
score 13.211869

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