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Evaluation of microbial water quality in recreational area in Kuching area

This study was carried out to monitor microbiological water quality in two different recreational areas around Kuching area. Rachan waterfall and UNIMAS lake were chosen to determine the presence of indicator microorganisms such as Escherichia coli. Most probable number (MPN) was evaluated for detec...

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Bibliographic Details
Main Author: Nadira, Binti Azuar
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2013
Subjects:
Q Science (General)
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/7525/8/Evaluation%20of%20Microbial%20Water%20Quality%20in%20Recreational%20Area%20in%20Kuching%20Area%28OCR%29.pdf
http://ir.unimas.my/id/eprint/7525/
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spelling my.unimas.ir.75252023-11-13T06:57:25Z http://ir.unimas.my/id/eprint/7525/ Evaluation of microbial water quality in recreational area in Kuching area Nadira, Binti Azuar Q Science (General) R Medicine (General) This study was carried out to monitor microbiological water quality in two different recreational areas around Kuching area. Rachan waterfall and UNIMAS lake were chosen to determine the presence of indicator microorganisms such as Escherichia coli. Most probable number (MPN) was evaluated for detecting and enumerating E.coli in water samples. Temperature and pH were used as parameters to evaluate the correlation towards distribution of coliforms in water samples. The positive tubes were cultured on eosin methylene blue agar (EMBA) for detection of E.coli. A total of 30 isolates of E.coli were isolated from both recreational areas. The representative isolates were tested through gram staining and standard biochemical tests. Out of 30, 26 were confirmed as E.coli based on the morphological characteristics. The isolates were tested for the presence of pathogenic Escherichia coli O157:H7 strain by multiplex PCR method targeting the stx1and stx2 genes. The stx1 and stx2 genes were not detected in any of the E.coli isolates. This study showed that there are no pathogenic strains found in these recreational areas and it safe for human to do recreational activity. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7525/8/Evaluation%20of%20Microbial%20Water%20Quality%20in%20Recreational%20Area%20in%20Kuching%20Area%28OCR%29.pdf Nadira, Binti Azuar (2013) Evaluation of microbial water quality in recreational area in Kuching area. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
R Medicine (General)
spellingShingle Q Science (General)
R Medicine (General)
Nadira, Binti Azuar
Evaluation of microbial water quality in recreational area in Kuching area
description This study was carried out to monitor microbiological water quality in two different recreational areas around Kuching area. Rachan waterfall and UNIMAS lake were chosen to determine the presence of indicator microorganisms such as Escherichia coli. Most probable number (MPN) was evaluated for detecting and enumerating E.coli in water samples. Temperature and pH were used as parameters to evaluate the correlation towards distribution of coliforms in water samples. The positive tubes were cultured on eosin methylene blue agar (EMBA) for detection of E.coli. A total of 30 isolates of E.coli were isolated from both recreational areas. The representative isolates were tested through gram staining and standard biochemical tests. Out of 30, 26 were confirmed as E.coli based on the morphological characteristics. The isolates were tested for the presence of pathogenic Escherichia coli O157:H7 strain by multiplex PCR method targeting the stx1and stx2 genes. The stx1 and stx2 genes were not detected in any of the E.coli isolates. This study showed that there are no pathogenic strains found in these recreational areas and it safe for human to do recreational activity.
format Final Year Project Report
author Nadira, Binti Azuar
author_facet Nadira, Binti Azuar
author_sort Nadira, Binti Azuar
title Evaluation of microbial water quality in recreational area in Kuching area
title_short Evaluation of microbial water quality in recreational area in Kuching area
title_full Evaluation of microbial water quality in recreational area in Kuching area
title_fullStr Evaluation of microbial water quality in recreational area in Kuching area
title_full_unstemmed Evaluation of microbial water quality in recreational area in Kuching area
title_sort evaluation of microbial water quality in recreational area in kuching area
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2013
url http://ir.unimas.my/id/eprint/7525/8/Evaluation%20of%20Microbial%20Water%20Quality%20in%20Recreational%20Area%20in%20Kuching%20Area%28OCR%29.pdf
http://ir.unimas.my/id/eprint/7525/
_version_ 1783883463909179392
score 13.211869

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