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Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography

Cyanobacterial toxicity was both tested from the natural water samples and isolated cyanobacterial samples collected from aquaculture ponds in Indigenous Fisheries Research and Production Center (IFRPC) Tarat, Serian. Water quality parameters such as salinity, turbidity, dissolved oxygen, pH and te...

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Main Author: Lai, Jia Rhou
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2011
Subjects:
QD Chemistry
TD Environmental technology. Sanitary engineering
Online Access:http://ir.unimas.my/id/eprint/7349/3/Lai%20Jia%20Rhou.pdf
http://ir.unimas.my/id/eprint/7349/
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spelling my.unimas.ir.73492023-12-13T08:55:36Z http://ir.unimas.my/id/eprint/7349/ Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography Lai, Jia Rhou QD Chemistry TD Environmental technology. Sanitary engineering Cyanobacterial toxicity was both tested from the natural water samples and isolated cyanobacterial samples collected from aquaculture ponds in Indigenous Fisheries Research and Production Center (IFRPC) Tarat, Serian. Water quality parameters such as salinity, turbidity, dissolved oxygen, pH and temperature were taken. Anabaena sp. was isolated and mass cultured by using medium ASN3. The cells were harvested at their late exponential phase of growth. Crude extracts were obtained from natural samples and Anabaena sp. by using 95% methanol. Brine shrimp (Artemia salina) bioassay was carried out on Anabaena strain by using eight concentrations (2, 5, 10, 20, 30, 40, 50, 100 g/ml) in filtered seawater. Endpoint of the experiment was mortality of brine shrimp. LC50 value obtained was approximately 8.32 g/ml and lethality at 30 g/ml. Thinlayer chromatography (TLC) and high performance liquid chromatography (HPLC) were used for further examination of microcystin by using standard of microcystin-LR. Microcystin were not detected in all of the samples, however, other toxins such as neurotoxin are expected to be available based on the mortality of the brine shrimps, retention factor values in TLC and overlay of peaks at 5.0 minutes retention times shown in HPLC chromatogram. Universiti Malaysia Sarawak, UNIMAS 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/7349/3/Lai%20Jia%20Rhou.pdf Lai, Jia Rhou (2011) Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
TD Environmental technology. Sanitary engineering
spellingShingle QD Chemistry
TD Environmental technology. Sanitary engineering
Lai, Jia Rhou
Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
description Cyanobacterial toxicity was both tested from the natural water samples and isolated cyanobacterial samples collected from aquaculture ponds in Indigenous Fisheries Research and Production Center (IFRPC) Tarat, Serian. Water quality parameters such as salinity, turbidity, dissolved oxygen, pH and temperature were taken. Anabaena sp. was isolated and mass cultured by using medium ASN3. The cells were harvested at their late exponential phase of growth. Crude extracts were obtained from natural samples and Anabaena sp. by using 95% methanol. Brine shrimp (Artemia salina) bioassay was carried out on Anabaena strain by using eight concentrations (2, 5, 10, 20, 30, 40, 50, 100 g/ml) in filtered seawater. Endpoint of the experiment was mortality of brine shrimp. LC50 value obtained was approximately 8.32 g/ml and lethality at 30 g/ml. Thinlayer chromatography (TLC) and high performance liquid chromatography (HPLC) were used for further examination of microcystin by using standard of microcystin-LR. Microcystin were not detected in all of the samples, however, other toxins such as neurotoxin are expected to be available based on the mortality of the brine shrimps, retention factor values in TLC and overlay of peaks at 5.0 minutes retention times shown in HPLC chromatogram.
format Final Year Project Report
author Lai, Jia Rhou
author_facet Lai, Jia Rhou
author_sort Lai, Jia Rhou
title Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
title_short Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
title_full Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
title_fullStr Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
title_full_unstemmed Toxicity Assessment of Cultured Cyanobacteria Toxin by Using Bioassay (Brine Shrimp, Artemia Salina), Thin Layer and High Performance Liquid Chromatography
title_sort toxicity assessment of cultured cyanobacteria toxin by using bioassay (brine shrimp, artemia salina), thin layer and high performance liquid chromatography
publisher Universiti Malaysia Sarawak, UNIMAS
publishDate 2011
url http://ir.unimas.my/id/eprint/7349/3/Lai%20Jia%20Rhou.pdf
http://ir.unimas.my/id/eprint/7349/
_version_ 1787140467279790080
score 13.211869

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