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Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba

Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable...

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Main Authors: Wei-Seng, Ho, Shek-Ling, Pang, Wee-Yang, C, M., Sim
Format: E-Article
Language:English
Published: International Academy Publishing (IAP) 2014
Subjects:
Q Science (General)
QD Chemistry
SD Forestry
Online Access:http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf
http://ir.unimas.my/id/eprint/7298/
http://www.ijbbb.org/show-49-727-1.html
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spelling my.unimas.ir.72982015-09-14T03:12:38Z http://ir.unimas.my/id/eprint/7298/ Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba Wei-Seng, Ho Shek-Ling, Pang Wee-Yang, C M., Sim Q Science (General) QD Chemistry SD Forestry Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable region II (HVRII) component involved in wood formation of Kelampayan is imperative for future applications. This region is thought to play a role in interaction with other unique cell-type-specific proteins involved in the biosynthesis of cellulose. The aim of this study was to identify and clone the HVRII regions of cellulose synthase gene from the developing xylem tissues of Kelampayan. The cDNA of cellulose synthase HVRII regions was amplified using reverse transcription-PCR (RT-PCR) approach using degenerate primers. Three clones, namely NcCesA1HVRII (520bp), NcCesA2HVRII (580bp) and NcCesA3HVRII (620bp) were successfully sequenced and characterized. NcCesA1HVRII and NcCesA3HVRII were clustered into two distinct clades implicated with secondary cell wall development whereas NcCesA2HVRII has renamed to NcCslD1HVRII due its high similarity with various plants’ CslD-HVRII. This study provides an easier and faster access to NcCesAHVRII sequences to further understand the role of NcCesA/NcCslD protein for future applications such as selecting trees with optimal cellulose content required for specific industries. International Academy Publishing (IAP) 2014 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf Wei-Seng, Ho and Shek-Ling, Pang and Wee-Yang, C and M., Sim (2014) Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba. International Journal of Bioscience, Biochemistry and Bioinformatics, 4 (6). pp. 475-482. ISSN 2010-3638 http://www.ijbbb.org/show-49-727-1.html doi: 10.7763/ijbbb.2014.v4.387
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QD Chemistry
SD Forestry
spellingShingle Q Science (General)
QD Chemistry
SD Forestry
Wei-Seng, Ho
Shek-Ling, Pang
Wee-Yang, C
M., Sim
Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
description Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable region II (HVRII) component involved in wood formation of Kelampayan is imperative for future applications. This region is thought to play a role in interaction with other unique cell-type-specific proteins involved in the biosynthesis of cellulose. The aim of this study was to identify and clone the HVRII regions of cellulose synthase gene from the developing xylem tissues of Kelampayan. The cDNA of cellulose synthase HVRII regions was amplified using reverse transcription-PCR (RT-PCR) approach using degenerate primers. Three clones, namely NcCesA1HVRII (520bp), NcCesA2HVRII (580bp) and NcCesA3HVRII (620bp) were successfully sequenced and characterized. NcCesA1HVRII and NcCesA3HVRII were clustered into two distinct clades implicated with secondary cell wall development whereas NcCesA2HVRII has renamed to NcCslD1HVRII due its high similarity with various plants’ CslD-HVRII. This study provides an easier and faster access to NcCesAHVRII sequences to further understand the role of NcCesA/NcCslD protein for future applications such as selecting trees with optimal cellulose content required for specific industries.
format E-Article
author Wei-Seng, Ho
Shek-Ling, Pang
Wee-Yang, C
M., Sim
author_facet Wei-Seng, Ho
Shek-Ling, Pang
Wee-Yang, C
M., Sim
author_sort Wei-Seng, Ho
title Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
title_short Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
title_full Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
title_fullStr Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
title_full_unstemmed Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
title_sort molecular cloning of hypervariable regions (hvrii) from cellulose synthase (cesa) gene in neolamarckia cadamba
publisher International Academy Publishing (IAP)
publishDate 2014
url http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf
http://ir.unimas.my/id/eprint/7298/
http://www.ijbbb.org/show-49-727-1.html
_version_ 1644510252816662528
score 13.211869

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